The protective effect of diosmin on hepatic ischemia reperfusion injury: an experimental study
Liver ischemia reperfusion injury (IRI) is an important pathologic process leading to bodily systemic effects and liver injury. Our study aimed to investigate the protective effects of diosmin, a phlebotrophic drug with antioxidant and anti-inflammatory effects, in a liver IRI model. Forty rats wer...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2013-11-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2305 |
| _version_ | 1827317396986134528 |
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| author | Yusuf Tanrikulu Mefaret Şahin Kemal Kismet Sibel Serin Kilicoglu Erdinc Devrim Ceren Sen Tanrikulu Esra Erdemli Serap Erel Kenan Bayraktar Mehmet Ali Akkus |
| author_facet | Yusuf Tanrikulu Mefaret Şahin Kemal Kismet Sibel Serin Kilicoglu Erdinc Devrim Ceren Sen Tanrikulu Esra Erdemli Serap Erel Kenan Bayraktar Mehmet Ali Akkus |
| author_sort | Yusuf Tanrikulu |
| collection | DOAJ |
| description |
Liver ischemia reperfusion injury (IRI) is an important pathologic process leading to bodily systemic effects and liver injury. Our study aimed to investigate the protective effects of diosmin, a phlebotrophic drug with antioxidant and anti-inflammatory effects, in a liver IRI model. Forty rats were divided into 4 groups. Sham group, control group (ischemia-reperfusion), intraoperative treatment group, and preoperative treatment group. Ischemia reperfusion model was formed by clamping hepatic pedicle for a 60 minute of ischemia followed by liver reperfusion for another 90 minutes. Superoxide dismutase (SOD) and catalase (CAT) were measured as antioaxidant enzymes in the liver tissues, and malondialdehyde (MDA) as oxidative stress marker, xanthine oxidase (XO) as an oxidant enzyme and glutathione peroxidase (GSH-Px) as antioaxidant enzyme were measured in the liver tissues and the plasma samples. Hepatic function tests were lower in treatment groups than control group (p<0.001 for ALT and AST). Plasma XO and MDA levels were lower in treatment groups than control group, but plasma GSH-Px levels were higher (p<0.05 for all). Tissue MDA levels were lower in treatment groups than control group, but tissue GSH-Px, SOD, CAT and XO levels were higher (p<0.05 for MDA and p<0.001 for others). Samples in control group histopathologically showed morphologic abnormalities specific to ischemia reperfusion. It has been found that both preoperative and intraoperative diosmin treatment decreases cellular damage and protects cells from toxic effects in liver IRI. As a conclusion, diosmin may be used as a protective agent against IRI in elective and emergent liver surgical operations.
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| first_indexed | 2024-04-24T23:32:42Z |
| format | Article |
| id | doaj.art-aba43ebfa1294a78aaf8d1f6e2eea99e |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:42Z |
| publishDate | 2013-11-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-aba43ebfa1294a78aaf8d1f6e2eea99e2024-03-15T14:32:53ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2013-11-0113410.17305/bjbms.2013.2305205The protective effect of diosmin on hepatic ischemia reperfusion injury: an experimental studyYusuf Tanrikulu0Mefaret Şahin1Kemal Kismet2Sibel Serin Kilicoglu3Erdinc Devrim4Ceren Sen Tanrikulu5Esra Erdemli6Serap Erel7Kenan Bayraktar8Mehmet Ali Akkus9Department of General Surgery, Ankara Training and Research Hospital, Ministry of HealthDepartment of General Surgery, Ankara Training and Research Hospital, Ministry of HealthDepartment of General Surgery, Ankara Training and Research Hospital, Ministry of HealthDepartment of Histology and Embriology, Faculty of Medicine, Ufuk UniversityDepartment of Biochemistry, Faculty of Medicine, Ankara UniversityDepartment of Emergency Medicine, Ankara Training and Research Hospital, Ministry of HealthDepartment of Histology and Embriology, Faculty of Medicine, Ankara UniversityDepartment of General Surgery, Ankara Training and Research Hospital, Ministry of HealthDepartment of General Surgery, Ankara Training and Research Hospital, Ministry of HealthDepartment of General Surgery, Ankara Training and Research Hospital, Ministry of Health Liver ischemia reperfusion injury (IRI) is an important pathologic process leading to bodily systemic effects and liver injury. Our study aimed to investigate the protective effects of diosmin, a phlebotrophic drug with antioxidant and anti-inflammatory effects, in a liver IRI model. Forty rats were divided into 4 groups. Sham group, control group (ischemia-reperfusion), intraoperative treatment group, and preoperative treatment group. Ischemia reperfusion model was formed by clamping hepatic pedicle for a 60 minute of ischemia followed by liver reperfusion for another 90 minutes. Superoxide dismutase (SOD) and catalase (CAT) were measured as antioaxidant enzymes in the liver tissues, and malondialdehyde (MDA) as oxidative stress marker, xanthine oxidase (XO) as an oxidant enzyme and glutathione peroxidase (GSH-Px) as antioaxidant enzyme were measured in the liver tissues and the plasma samples. Hepatic function tests were lower in treatment groups than control group (p<0.001 for ALT and AST). Plasma XO and MDA levels were lower in treatment groups than control group, but plasma GSH-Px levels were higher (p<0.05 for all). Tissue MDA levels were lower in treatment groups than control group, but tissue GSH-Px, SOD, CAT and XO levels were higher (p<0.05 for MDA and p<0.001 for others). Samples in control group histopathologically showed morphologic abnormalities specific to ischemia reperfusion. It has been found that both preoperative and intraoperative diosmin treatment decreases cellular damage and protects cells from toxic effects in liver IRI. As a conclusion, diosmin may be used as a protective agent against IRI in elective and emergent liver surgical operations. https://www.bjbms.org/ojs/index.php/bjbms/article/view/2305diosminischemia-reperfusionflavonoidliver |
| spellingShingle | Yusuf Tanrikulu Mefaret Şahin Kemal Kismet Sibel Serin Kilicoglu Erdinc Devrim Ceren Sen Tanrikulu Esra Erdemli Serap Erel Kenan Bayraktar Mehmet Ali Akkus The protective effect of diosmin on hepatic ischemia reperfusion injury: an experimental study Biomolecules & Biomedicine diosmin ischemia-reperfusion flavonoid liver |
| title | The protective effect of diosmin on hepatic ischemia reperfusion injury: an experimental study |
| title_full | The protective effect of diosmin on hepatic ischemia reperfusion injury: an experimental study |
| title_fullStr | The protective effect of diosmin on hepatic ischemia reperfusion injury: an experimental study |
| title_full_unstemmed | The protective effect of diosmin on hepatic ischemia reperfusion injury: an experimental study |
| title_short | The protective effect of diosmin on hepatic ischemia reperfusion injury: an experimental study |
| title_sort | protective effect of diosmin on hepatic ischemia reperfusion injury an experimental study |
| topic | diosmin ischemia-reperfusion flavonoid liver |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2305 |
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