Isolation and identification of Bacillus producing thermophilic alpha amylase: production optimization and investigation of the activity and stability of enzyme.
Alpha-amylases are the most important amylases used in industry. Among them, thermophilic alpha-amylases are of particular importance, which is due to their activity and stability in high temperatures. These enzymes produced by thermophile micro-organisms including bacteria. These thermophilic alpha...
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| Format: | Article |
| Language: | English |
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Kharazmi University
2018-03-01
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| Series: | یافتههای نوین در علوم زیستی |
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| Online Access: | http://nbr.khu.ac.ir/browse.php?a_code=A-10-81-95&slc_lang=en&sid=1 |
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| author | Saide Afrisham Arastoo Badoei-delfard Abdolhamid Namaki Shoushtari Zahra Karami Saeid Malek-abadi |
| author_facet | Saide Afrisham Arastoo Badoei-delfard Abdolhamid Namaki Shoushtari Zahra Karami Saeid Malek-abadi |
| author_sort | Saide Afrisham |
| collection | DOAJ |
| description | Alpha-amylases are the most important amylases used in industry. Among them, thermophilic alpha-amylases are of particular importance, which is due to their activity and stability in high temperatures. These enzymes produced by thermophile micro-organisms including bacteria. These thermophilic alpha-amylases are used in various industries such as processing of starch as well as production of detergents and biofuels. In this research, the bacteria which produce the thermophilic alpha-amylases were isolated and characterized in hot springs of Gorooh village in Kerman province. According to the results of screening on the specific liquid and solid media, AT59 was selected as the best strain. Morphological and biochemical characterization of the isolated strain indicated that it belonged to Bacillus sp. and was gram-positive, catalase positive, casein hydrolyzing and acid producing from lactose and sucrose. The results obtained from the optimization of the enzyme production medium showed that among the carbon, nitrogen and ion sources investigated, starch (1 gr/l), gelatin (2 g/l) and magnesium sulfate (1 g/l) had the most increasing effect on the production of AT59 alpha-amylase. Moreover, the highest enzyme production was obtained at pH 5. This enzyme also demonstrated the highest degree of activity and stability in 80 and 70 ℃, respectively. These findings suggested that this enzyme has a considerable potential for use in starch industry. |
| first_indexed | 2024-04-13T14:51:52Z |
| format | Article |
| id | doaj.art-aba57bb779554b8d8b513a96ff5904e2 |
| institution | Directory Open Access Journal |
| issn | 2423-6330 2476-7115 |
| language | English |
| last_indexed | 2024-04-13T14:51:52Z |
| publishDate | 2018-03-01 |
| publisher | Kharazmi University |
| record_format | Article |
| series | یافتههای نوین در علوم زیستی |
| spelling | doaj.art-aba57bb779554b8d8b513a96ff5904e22022-12-22T02:42:35ZengKharazmi Universityیافتههای نوین در علوم زیستی2423-63302476-71152018-03-0144288298Isolation and identification of Bacillus producing thermophilic alpha amylase: production optimization and investigation of the activity and stability of enzyme.Saide Afrisham0Arastoo Badoei-delfard1Abdolhamid Namaki Shoushtari2Zahra Karami3Saeid Malek-abadi4 Shahid Bahonar University of Kerman Shahid Bahonar University of Kerman Shahid Bahonar University of Kerman Shahid Bahonar University of Kerman Alpha-amylases are the most important amylases used in industry. Among them, thermophilic alpha-amylases are of particular importance, which is due to their activity and stability in high temperatures. These enzymes produced by thermophile micro-organisms including bacteria. These thermophilic alpha-amylases are used in various industries such as processing of starch as well as production of detergents and biofuels. In this research, the bacteria which produce the thermophilic alpha-amylases were isolated and characterized in hot springs of Gorooh village in Kerman province. According to the results of screening on the specific liquid and solid media, AT59 was selected as the best strain. Morphological and biochemical characterization of the isolated strain indicated that it belonged to Bacillus sp. and was gram-positive, catalase positive, casein hydrolyzing and acid producing from lactose and sucrose. The results obtained from the optimization of the enzyme production medium showed that among the carbon, nitrogen and ion sources investigated, starch (1 gr/l), gelatin (2 g/l) and magnesium sulfate (1 g/l) had the most increasing effect on the production of AT59 alpha-amylase. Moreover, the highest enzyme production was obtained at pH 5. This enzyme also demonstrated the highest degree of activity and stability in 80 and 70 ℃, respectively. These findings suggested that this enzyme has a considerable potential for use in starch industry.http://nbr.khu.ac.ir/browse.php?a_code=A-10-81-95&slc_lang=en&sid=1hot springproductionscreeningthermophilic enzymes |
| spellingShingle | Saide Afrisham Arastoo Badoei-delfard Abdolhamid Namaki Shoushtari Zahra Karami Saeid Malek-abadi Isolation and identification of Bacillus producing thermophilic alpha amylase: production optimization and investigation of the activity and stability of enzyme. یافتههای نوین در علوم زیستی hot spring production screening thermophilic enzymes |
| title | Isolation and identification of Bacillus producing thermophilic alpha amylase: production optimization and investigation of the activity and stability of enzyme. |
| title_full | Isolation and identification of Bacillus producing thermophilic alpha amylase: production optimization and investigation of the activity and stability of enzyme. |
| title_fullStr | Isolation and identification of Bacillus producing thermophilic alpha amylase: production optimization and investigation of the activity and stability of enzyme. |
| title_full_unstemmed | Isolation and identification of Bacillus producing thermophilic alpha amylase: production optimization and investigation of the activity and stability of enzyme. |
| title_short | Isolation and identification of Bacillus producing thermophilic alpha amylase: production optimization and investigation of the activity and stability of enzyme. |
| title_sort | isolation and identification of bacillus producing thermophilic alpha amylase production optimization and investigation of the activity and stability of enzyme |
| topic | hot spring production screening thermophilic enzymes |
| url | http://nbr.khu.ac.ir/browse.php?a_code=A-10-81-95&slc_lang=en&sid=1 |
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