Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice
In order to effectively deliver lutein to the inflamed colon and better exert its pharmacological activity, this paper constructed a sodium alginate hydrogel-based delivery system loaded with lutein nanoparticles, evaluated the regulation on the expression and secretion of related inflammatory facto...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000629 |
| _version_ | 1797703403842830336 |
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| author | Pengxiang Xu Shuwei Luo Jiangfeng Song Zhuqing Dai Dajing Li Cai’e Wu |
| author_facet | Pengxiang Xu Shuwei Luo Jiangfeng Song Zhuqing Dai Dajing Li Cai’e Wu |
| author_sort | Pengxiang Xu |
| collection | DOAJ |
| description | In order to effectively deliver lutein to the inflamed colon and better exert its pharmacological activity, this paper constructed a sodium alginate hydrogel-based delivery system loaded with lutein nanoparticles, evaluated the regulation on the expression and secretion of related inflammatory factors in mice with colitis, and its impact on intestinal microbial environment. The results showed that comparing lutein crystal and its nanoparticle, lutein hydrogel alleviated dextran sodium sulfate (DSS)-induced colitis in mice more effectively by adjusting fecal heme content, colon tissue damage, and inflammatory factor levels. Moreover, lutein hydrogel increased the expression of intestinal tight junction proteins zonula occluden-1 (ZO-1), claudin-1 and occludin to maintain the integrity of the intestinal-barrier, inhibited the nuclear factor-κB (NF-κB) pathway and reduced expression and secretion of inflammatory factors including tumour necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), NOD-like receptors 3 (NLRP3) and interleukin-1β (IL-1β). In addition, the intestinal microbial environment of mice with colitis was improved by down-regulating the relative abundance of Desulfovibrionaceae and up-regulating the relative abundance of Erysipelotrichaceae and Rikenellaceae. As a slow-release carrier to load lutein nanoparticles, sodium alginate-based hydrogel has potential application prospect. |
| first_indexed | 2024-03-12T05:05:04Z |
| format | Article |
| id | doaj.art-aba5e4fc88864c45adfaf0bed3865b53 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T05:05:04Z |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-aba5e4fc88864c45adfaf0bed3865b532023-09-03T09:00:35ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112624282439Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis micePengxiang Xu0Shuwei Luo1Jiangfeng Song2Zhuqing Dai3Dajing Li4Cai’e Wu5Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, ChinaInstitute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, ChinaInstitute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China; Corresponding author at: Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaCollege of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, ChinaIn order to effectively deliver lutein to the inflamed colon and better exert its pharmacological activity, this paper constructed a sodium alginate hydrogel-based delivery system loaded with lutein nanoparticles, evaluated the regulation on the expression and secretion of related inflammatory factors in mice with colitis, and its impact on intestinal microbial environment. The results showed that comparing lutein crystal and its nanoparticle, lutein hydrogel alleviated dextran sodium sulfate (DSS)-induced colitis in mice more effectively by adjusting fecal heme content, colon tissue damage, and inflammatory factor levels. Moreover, lutein hydrogel increased the expression of intestinal tight junction proteins zonula occluden-1 (ZO-1), claudin-1 and occludin to maintain the integrity of the intestinal-barrier, inhibited the nuclear factor-κB (NF-κB) pathway and reduced expression and secretion of inflammatory factors including tumour necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), NOD-like receptors 3 (NLRP3) and interleukin-1β (IL-1β). In addition, the intestinal microbial environment of mice with colitis was improved by down-regulating the relative abundance of Desulfovibrionaceae and up-regulating the relative abundance of Erysipelotrichaceae and Rikenellaceae. As a slow-release carrier to load lutein nanoparticles, sodium alginate-based hydrogel has potential application prospect.http://www.sciencedirect.com/science/article/pii/S2213453023000629LuteinHydrogelColitisIntestinal microbiotaInflammation regulation |
| spellingShingle | Pengxiang Xu Shuwei Luo Jiangfeng Song Zhuqing Dai Dajing Li Cai’e Wu Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice Food Science and Human Wellness Lutein Hydrogel Colitis Intestinal microbiota Inflammation regulation |
| title | Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice |
| title_full | Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice |
| title_fullStr | Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice |
| title_full_unstemmed | Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice |
| title_short | Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice |
| title_sort | effect of sodium alginate based hydrogel loaded with lutein on gut microbiota and inflammatory response in dss induced colitis mice |
| topic | Lutein Hydrogel Colitis Intestinal microbiota Inflammation regulation |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000629 |
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