Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid
Anti-laminin 332 mucous membrane pemphigoid (MMP) is an autoimmune blistering disease characterized by predominant mucosal lesions and autoantibodies against laminin 332. The exact diagnosis of anti-laminin 332 MMP is important since nearly 30% of patients develop solid cancers. This study compared...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.773720/full |
| _version_ | 1818425747950272512 |
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| author | Stephanie Goletz Federica Giurdanella Maike M. Holtsche Miranda Nijenhuis Barbara Horvath Gilles F. H. Diercks Detlef Zillikens Takashi Hashimoto Enno Schmidt Enno Schmidt Hendri H. Pas |
| author_facet | Stephanie Goletz Federica Giurdanella Maike M. Holtsche Miranda Nijenhuis Barbara Horvath Gilles F. H. Diercks Detlef Zillikens Takashi Hashimoto Enno Schmidt Enno Schmidt Hendri H. Pas |
| author_sort | Stephanie Goletz |
| collection | DOAJ |
| description | Anti-laminin 332 mucous membrane pemphigoid (MMP) is an autoimmune blistering disease characterized by predominant mucosal lesions and autoantibodies against laminin 332. The exact diagnosis of anti-laminin 332 MMP is important since nearly 30% of patients develop solid cancers. This study compared two independently developed diagnostic indirect immunofluorescence (IF) tests based on recombinant laminin 332 expressed in HEK239 cells (biochip mosaic assay) and the migration trails of cultured keratinocytes rich in laminin 332 (footprint assay). The sera of 54 anti-laminin 332 MMP, 35 non-anti-laminin 332 MMP, and 30 pemphigus vulgaris patients as well as 20 healthy blood donors were analyzed blindly and independently. Fifty-two of 54 and 54/54 anti-laminin 332 MMP sera were positive in the biochip mosaic and the footprint assay, respectively. In the 35 non-anti-laminin 332 MMP sera, 3 were positive in both tests and 4 others showed weak reactivity in the footprint assay. In conclusion, both assays are easy to perform, highly sensitive, and specific, which will further facilitate the diagnosis of anti-laminin 332 MMP. |
| first_indexed | 2024-12-14T14:18:52Z |
| format | Article |
| id | doaj.art-ababc5e370bd45299b68ae0f420b8693 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-14T14:18:52Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-ababc5e370bd45299b68ae0f420b86932022-12-21T22:58:08ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-11-011210.3389/fimmu.2021.773720773720Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane PemphigoidStephanie Goletz0Federica Giurdanella1Maike M. Holtsche2Miranda Nijenhuis3Barbara Horvath4Gilles F. H. Diercks5Detlef Zillikens6Takashi Hashimoto7Enno Schmidt8Enno Schmidt9Hendri H. Pas10Lübeck Institute for Experimental Dermatology (LIED), University of Lübeck, Lübeck, GermanyCenter for Blistering Diseases, University of Groningen, Groningen, NetherlandsDepartment of Dermatology, University of Lübeck, Lübeck, GermanyCenter for Blistering Diseases, University of Groningen, Groningen, NetherlandsCenter for Blistering Diseases, University of Groningen, Groningen, NetherlandsCenter for Blistering Diseases, University of Groningen, Groningen, NetherlandsDepartment of Dermatology, University of Lübeck, Lübeck, GermanyDepartment of Dermatology, Kurume University School of Medicine, Kurume, JapanLübeck Institute for Experimental Dermatology (LIED), University of Lübeck, Lübeck, GermanyDepartment of Dermatology, University of Lübeck, Lübeck, GermanyCenter for Blistering Diseases, University of Groningen, Groningen, NetherlandsAnti-laminin 332 mucous membrane pemphigoid (MMP) is an autoimmune blistering disease characterized by predominant mucosal lesions and autoantibodies against laminin 332. The exact diagnosis of anti-laminin 332 MMP is important since nearly 30% of patients develop solid cancers. This study compared two independently developed diagnostic indirect immunofluorescence (IF) tests based on recombinant laminin 332 expressed in HEK239 cells (biochip mosaic assay) and the migration trails of cultured keratinocytes rich in laminin 332 (footprint assay). The sera of 54 anti-laminin 332 MMP, 35 non-anti-laminin 332 MMP, and 30 pemphigus vulgaris patients as well as 20 healthy blood donors were analyzed blindly and independently. Fifty-two of 54 and 54/54 anti-laminin 332 MMP sera were positive in the biochip mosaic and the footprint assay, respectively. In the 35 non-anti-laminin 332 MMP sera, 3 were positive in both tests and 4 others showed weak reactivity in the footprint assay. In conclusion, both assays are easy to perform, highly sensitive, and specific, which will further facilitate the diagnosis of anti-laminin 332 MMP.https://www.frontiersin.org/articles/10.3389/fimmu.2021.773720/fullautoantibodyblisteringbiochipcancerfootprintlaminin 332 |
| spellingShingle | Stephanie Goletz Federica Giurdanella Maike M. Holtsche Miranda Nijenhuis Barbara Horvath Gilles F. H. Diercks Detlef Zillikens Takashi Hashimoto Enno Schmidt Enno Schmidt Hendri H. Pas Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid Frontiers in Immunology autoantibody blistering biochip cancer footprint laminin 332 |
| title | Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid |
| title_full | Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid |
| title_fullStr | Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid |
| title_full_unstemmed | Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid |
| title_short | Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid |
| title_sort | comparison of two diagnostic assays for anti laminin 332 mucous membrane pemphigoid |
| topic | autoantibody blistering biochip cancer footprint laminin 332 |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.773720/full |
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