Adaptation of winged bean (Psophocarpus tetragonolobus (L.) DC.) to drought stress is mediated by root-tuber heat-shock proteins and specific metabolites
Winged bean (Psophocarpus tetragonolobus (L.) DC.) is an underexploited tropical legume whose tuberous-roots have 12–19% proteins. The species has excellent drought tolerance ability thus making it a prospective climate-smart future crop. However, the underlying defense mechanism(s) of the species a...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Current Plant Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662822000330 |
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| author | Rayees Ahmad Lone Kunwar Sarvendra Vinayak Singh Nasreen Bano Sumit Kumar Bag Chandra Sekhar Mohanty Saroj Kanta Barik |
| author_facet | Rayees Ahmad Lone Kunwar Sarvendra Vinayak Singh Nasreen Bano Sumit Kumar Bag Chandra Sekhar Mohanty Saroj Kanta Barik |
| author_sort | Rayees Ahmad Lone |
| collection | DOAJ |
| description | Winged bean (Psophocarpus tetragonolobus (L.) DC.) is an underexploited tropical legume whose tuberous-roots have 12–19% proteins. The species has excellent drought tolerance ability thus making it a prospective climate-smart future crop. However, the underlying defense mechanism(s) of the species against the drought stress are not yet understood. Therefore, we studied the role of root- tuber proteins and metabolites in providing drought stress resistance to winged bean. We identified and characterized the relevant proteins and their corresponding peptides, and validated the responsible transcripts in root-tubers of P. tetragonolobus through qRT-PCR. Total proteome-analysis of root-tubers resulted in identification of 2678 proteins belonging to 154 families. Of these, 96% of proteins are associated with known biological activities. Heat-shock proteins (Hsps) of 12–42 kDa were the most abundant protein families and constituted 13% of the total identified proteins in the root-tuber. Metabolite analysis of the root-tuber revealed the prevalence of abiotic stress tolerant amino acids and compounds such as dimethyl amide, hydroxylamine hydrochloride, trimethylamine, cyclopropylamine, α-aminopropionitrile and cyclohexylamine. The other related metabolites present were naphthalene, benzyl alcohol, L- rhamnose, propanol and aromatic aldehydes. The presence of several Hsps, aminoacids, and specific metabolites with known function of stress-amelioration/anti-oxidant activities present in the root-tubers explains the adaptation success of winged bean against drought stress. |
| first_indexed | 2024-04-13T05:46:13Z |
| format | Article |
| id | doaj.art-abaf7daaa1c7405eb194629f39987cf8 |
| institution | Directory Open Access Journal |
| issn | 2214-6628 |
| language | English |
| last_indexed | 2024-04-13T05:46:13Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Plant Biology |
| spelling | doaj.art-abaf7daaa1c7405eb194629f39987cf82022-12-22T02:59:57ZengElsevierCurrent Plant Biology2214-66282022-12-0132100266Adaptation of winged bean (Psophocarpus tetragonolobus (L.) DC.) to drought stress is mediated by root-tuber heat-shock proteins and specific metabolitesRayees Ahmad Lone0Kunwar Sarvendra1Vinayak Singh2Nasreen Bano3Sumit Kumar Bag4Chandra Sekhar Mohanty5Saroj Kanta Barik6CSIR-National Botanical Research Institute, Lucknow, India; Academy of Scientific and Innovative Research, Ghaziabad, IndiaCSIR-National Botanical Research Institute, Lucknow, IndiaCSIR-National Botanical Research Institute, Lucknow, IndiaCSIR-National Botanical Research Institute, Lucknow, India; Academy of Scientific and Innovative Research, Ghaziabad, IndiaCSIR-National Botanical Research Institute, Lucknow, India; Academy of Scientific and Innovative Research, Ghaziabad, IndiaCSIR-National Botanical Research Institute, Lucknow, India; Academy of Scientific and Innovative Research, Ghaziabad, India; Correspondence to: CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India.CSIR-National Botanical Research Institute, Lucknow, India; Academy of Scientific and Innovative Research, Ghaziabad, India; Correspondence to: CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India.Winged bean (Psophocarpus tetragonolobus (L.) DC.) is an underexploited tropical legume whose tuberous-roots have 12–19% proteins. The species has excellent drought tolerance ability thus making it a prospective climate-smart future crop. However, the underlying defense mechanism(s) of the species against the drought stress are not yet understood. Therefore, we studied the role of root- tuber proteins and metabolites in providing drought stress resistance to winged bean. We identified and characterized the relevant proteins and their corresponding peptides, and validated the responsible transcripts in root-tubers of P. tetragonolobus through qRT-PCR. Total proteome-analysis of root-tubers resulted in identification of 2678 proteins belonging to 154 families. Of these, 96% of proteins are associated with known biological activities. Heat-shock proteins (Hsps) of 12–42 kDa were the most abundant protein families and constituted 13% of the total identified proteins in the root-tuber. Metabolite analysis of the root-tuber revealed the prevalence of abiotic stress tolerant amino acids and compounds such as dimethyl amide, hydroxylamine hydrochloride, trimethylamine, cyclopropylamine, α-aminopropionitrile and cyclohexylamine. The other related metabolites present were naphthalene, benzyl alcohol, L- rhamnose, propanol and aromatic aldehydes. The presence of several Hsps, aminoacids, and specific metabolites with known function of stress-amelioration/anti-oxidant activities present in the root-tubers explains the adaptation success of winged bean against drought stress.http://www.sciencedirect.com/science/article/pii/S2214662822000330Winged beanRoot-tuberProteomeHeat-shock proteinsDrought resistance |
| spellingShingle | Rayees Ahmad Lone Kunwar Sarvendra Vinayak Singh Nasreen Bano Sumit Kumar Bag Chandra Sekhar Mohanty Saroj Kanta Barik Adaptation of winged bean (Psophocarpus tetragonolobus (L.) DC.) to drought stress is mediated by root-tuber heat-shock proteins and specific metabolites Current Plant Biology Winged bean Root-tuber Proteome Heat-shock proteins Drought resistance |
| title | Adaptation of winged bean (Psophocarpus tetragonolobus (L.) DC.) to drought stress is mediated by root-tuber heat-shock proteins and specific metabolites |
| title_full | Adaptation of winged bean (Psophocarpus tetragonolobus (L.) DC.) to drought stress is mediated by root-tuber heat-shock proteins and specific metabolites |
| title_fullStr | Adaptation of winged bean (Psophocarpus tetragonolobus (L.) DC.) to drought stress is mediated by root-tuber heat-shock proteins and specific metabolites |
| title_full_unstemmed | Adaptation of winged bean (Psophocarpus tetragonolobus (L.) DC.) to drought stress is mediated by root-tuber heat-shock proteins and specific metabolites |
| title_short | Adaptation of winged bean (Psophocarpus tetragonolobus (L.) DC.) to drought stress is mediated by root-tuber heat-shock proteins and specific metabolites |
| title_sort | adaptation of winged bean psophocarpus tetragonolobus l dc to drought stress is mediated by root tuber heat shock proteins and specific metabolites |
| topic | Winged bean Root-tuber Proteome Heat-shock proteins Drought resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2214662822000330 |
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