Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery Products
Histamine poisoning is a significant public health problem. Therefore, the monitoring of histamine content in fish and fishery products is considered to be a crucial measure in the seafood industry. In the present study, a simple and rapid densitometric thin-layer chromatographic (TLC) method for hi...
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MDPI AG
2020-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/16/3611 |
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| author | Ayoub Kounnoun Adnane Louajri Francesco Cacciola Hafssa El Cadi Hajar Bougtaib Naoual Alahlah Aicha El Baaboua Mohamed El Maadoudi |
| author_facet | Ayoub Kounnoun Adnane Louajri Francesco Cacciola Hafssa El Cadi Hajar Bougtaib Naoual Alahlah Aicha El Baaboua Mohamed El Maadoudi |
| author_sort | Ayoub Kounnoun |
| collection | DOAJ |
| description | Histamine poisoning is a significant public health problem. Therefore, the monitoring of histamine content in fish and fishery products is considered to be a crucial measure in the seafood industry. In the present study, a simple and rapid densitometric thin-layer chromatographic (TLC) method for histamine determination in fish samples was developed and validated. The samples were homogenized with 10% trichloroacetic acid and histamine was efficiently extracted. Then, an appropriate derivatization procedure was adopted with dansyl chloride. Once the derivatization was carried out, the samples were applied to silica gel TLC plates and developed by ascending chromatography with chloroform-triethylamine (6:4, <i>v</i>/<i>v</i>) as the mobile phase. The intensity of the histamine-dansyl derivative spots was measured by densitometry at 365 nm, and the quantitation was performed by BIO-1D image processing software. The validation of this method revealed good linearity and specificity over a concentration range from 6.25 to 100 mg/kg. Adequate precision was shown by relative standard deviations (RSD) smaller than 4.82%, accuracy ranged from −6.88% to 5.28%, and satisfactory recoveries ranging from 93% to 105% were obtained. The Limit of Detection and the Limit of Quantification were calculated at 4.4 mg/kg and 10.5 mg/kg, respectively. In addition, the effectiveness of the proposed method was assessed by the analysis of various samples, and the obtained results were confirmed with those achieved by the HPLC-UV method. Moreover, the developed method was found to be simple, cheap, and suitable for application to analyze several samples simultaneously. |
| first_indexed | 2024-03-10T17:46:07Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T17:46:07Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-abafcbf76022407eb7b39b6a1ca1f5b22023-11-20T09:32:33ZengMDPI AGMolecules1420-30492020-08-012516361110.3390/molecules25163611Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery ProductsAyoub Kounnoun0Adnane Louajri1Francesco Cacciola2Hafssa El Cadi3Hajar Bougtaib4Naoual Alahlah5Aicha El Baaboua6Mohamed El Maadoudi7Laboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abd Al Malek Essaadi University, Tetouan 93000, MoroccoLaboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abd Al Malek Essaadi University, Tetouan 93000, MoroccoDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, ItalyLaboratory of Valorization of Resources and Chemical Engineering, Department of Chemistry, Abdelmalek Essaâdi University, Tangier 90000, MoroccoLaboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abd Al Malek Essaadi University, Tetouan 93000, MoroccoRegional Analysis and Research Laboratory, National Office of Food Safety ONSSA, Tangier 90000, MoroccoLaboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abd Al Malek Essaadi University, Tetouan 93000, MoroccoRegional Analysis and Research Laboratory, National Office of Food Safety ONSSA, Tangier 90000, MoroccoHistamine poisoning is a significant public health problem. Therefore, the monitoring of histamine content in fish and fishery products is considered to be a crucial measure in the seafood industry. In the present study, a simple and rapid densitometric thin-layer chromatographic (TLC) method for histamine determination in fish samples was developed and validated. The samples were homogenized with 10% trichloroacetic acid and histamine was efficiently extracted. Then, an appropriate derivatization procedure was adopted with dansyl chloride. Once the derivatization was carried out, the samples were applied to silica gel TLC plates and developed by ascending chromatography with chloroform-triethylamine (6:4, <i>v</i>/<i>v</i>) as the mobile phase. The intensity of the histamine-dansyl derivative spots was measured by densitometry at 365 nm, and the quantitation was performed by BIO-1D image processing software. The validation of this method revealed good linearity and specificity over a concentration range from 6.25 to 100 mg/kg. Adequate precision was shown by relative standard deviations (RSD) smaller than 4.82%, accuracy ranged from −6.88% to 5.28%, and satisfactory recoveries ranging from 93% to 105% were obtained. The Limit of Detection and the Limit of Quantification were calculated at 4.4 mg/kg and 10.5 mg/kg, respectively. In addition, the effectiveness of the proposed method was assessed by the analysis of various samples, and the obtained results were confirmed with those achieved by the HPLC-UV method. Moreover, the developed method was found to be simple, cheap, and suitable for application to analyze several samples simultaneously.https://www.mdpi.com/1420-3049/25/16/3611histaminefishTLCdensitometryoptimizationvalidation |
| spellingShingle | Ayoub Kounnoun Adnane Louajri Francesco Cacciola Hafssa El Cadi Hajar Bougtaib Naoual Alahlah Aicha El Baaboua Mohamed El Maadoudi Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery Products Molecules histamine fish TLC densitometry optimization validation |
| title | Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery Products |
| title_full | Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery Products |
| title_fullStr | Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery Products |
| title_full_unstemmed | Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery Products |
| title_short | Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery Products |
| title_sort | development and validation of a tlc densitometry method for histamine monitoring in fish and fishery products |
| topic | histamine fish TLC densitometry optimization validation |
| url | https://www.mdpi.com/1420-3049/25/16/3611 |
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