GABAergic CA1 neurons are more stable following context changes than glutamatergic cells
Abstract The CA1 region of the hippocampus contains both glutamatergic pyramidal cells and GABAergic interneurons. Numerous reports have characterized glutamatergic CAMK2A cell activity, showing how these cells respond to environmental changes such as local cue rotation and context re-sizing. Additi...
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| Format: | Article |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13799-6 |
| _version_ | 1811334578668830720 |
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| author | Peter J. Schuette Juliane M. Ikebara Sandra Maesta-Pereira Anita Torossian Ekayana Sethi Alexandre H. Kihara Jonathan C. Kao Fernando M. C. V. Reis Avishek Adhikari |
| author_facet | Peter J. Schuette Juliane M. Ikebara Sandra Maesta-Pereira Anita Torossian Ekayana Sethi Alexandre H. Kihara Jonathan C. Kao Fernando M. C. V. Reis Avishek Adhikari |
| author_sort | Peter J. Schuette |
| collection | DOAJ |
| description | Abstract The CA1 region of the hippocampus contains both glutamatergic pyramidal cells and GABAergic interneurons. Numerous reports have characterized glutamatergic CAMK2A cell activity, showing how these cells respond to environmental changes such as local cue rotation and context re-sizing. Additionally, the long-term stability of spatial encoding and turnover of these cells across days is also well-characterized. In contrast, these classic hippocampal experiments have never been conducted with CA1 GABAergic cells. Here, we use chronic calcium imaging of male and female mice to compare the neural activity of VGAT and CAMK2A cells during exploration of unaltered environments and also during exposure to contexts before and after rotating and changing the length of the context across multiple recording days. Intriguingly, compared to CAMK2A cells, VGAT cells showed decreased remapping induced by environmental changes, such as context rotations and contextual length resizing. However, GABAergic neurons were also less likely than glutamatergic neurons to remain active and exhibit consistent place coding across recording days. Interestingly, despite showing significant spatial remapping across days, GABAergic cells had stable speed encoding between days. Thus, compared to glutamatergic cells, spatial encoding of GABAergic cells is more stable during within-session environmental perturbations, but is less stable across days. These insights may be crucial in accurately modeling the features and constraints of hippocampal dynamics in spatial coding. |
| first_indexed | 2024-04-13T17:10:18Z |
| format | Article |
| id | doaj.art-abb180db018f4399a8b1a97c0182fe28 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T17:10:18Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-abb180db018f4399a8b1a97c0182fe282022-12-22T02:38:18ZengNature PortfolioScientific Reports2045-23222022-06-0112111810.1038/s41598-022-13799-6GABAergic CA1 neurons are more stable following context changes than glutamatergic cellsPeter J. Schuette0Juliane M. Ikebara1Sandra Maesta-Pereira2Anita Torossian3Ekayana Sethi4Alexandre H. Kihara5Jonathan C. Kao6Fernando M. C. V. Reis7Avishek Adhikari8Department of Psychology, University of California, Los AngelesCentro de Matemática, Computação e Cognição, Universidade Federal do ABCDepartment of Psychology, University of California, Los AngelesDepartment of Psychology, University of California, Los AngelesDepartment of Psychology, University of California, Los AngelesCentro de Matemática, Computação e Cognição, Universidade Federal do ABCDepartment of Electrical and Computer Engineering, University of California, Los AngelesDepartment of Psychology, University of California, Los AngelesDepartment of Psychology, University of California, Los AngelesAbstract The CA1 region of the hippocampus contains both glutamatergic pyramidal cells and GABAergic interneurons. Numerous reports have characterized glutamatergic CAMK2A cell activity, showing how these cells respond to environmental changes such as local cue rotation and context re-sizing. Additionally, the long-term stability of spatial encoding and turnover of these cells across days is also well-characterized. In contrast, these classic hippocampal experiments have never been conducted with CA1 GABAergic cells. Here, we use chronic calcium imaging of male and female mice to compare the neural activity of VGAT and CAMK2A cells during exploration of unaltered environments and also during exposure to contexts before and after rotating and changing the length of the context across multiple recording days. Intriguingly, compared to CAMK2A cells, VGAT cells showed decreased remapping induced by environmental changes, such as context rotations and contextual length resizing. However, GABAergic neurons were also less likely than glutamatergic neurons to remain active and exhibit consistent place coding across recording days. Interestingly, despite showing significant spatial remapping across days, GABAergic cells had stable speed encoding between days. Thus, compared to glutamatergic cells, spatial encoding of GABAergic cells is more stable during within-session environmental perturbations, but is less stable across days. These insights may be crucial in accurately modeling the features and constraints of hippocampal dynamics in spatial coding.https://doi.org/10.1038/s41598-022-13799-6 |
| spellingShingle | Peter J. Schuette Juliane M. Ikebara Sandra Maesta-Pereira Anita Torossian Ekayana Sethi Alexandre H. Kihara Jonathan C. Kao Fernando M. C. V. Reis Avishek Adhikari GABAergic CA1 neurons are more stable following context changes than glutamatergic cells Scientific Reports |
| title | GABAergic CA1 neurons are more stable following context changes than glutamatergic cells |
| title_full | GABAergic CA1 neurons are more stable following context changes than glutamatergic cells |
| title_fullStr | GABAergic CA1 neurons are more stable following context changes than glutamatergic cells |
| title_full_unstemmed | GABAergic CA1 neurons are more stable following context changes than glutamatergic cells |
| title_short | GABAergic CA1 neurons are more stable following context changes than glutamatergic cells |
| title_sort | gabaergic ca1 neurons are more stable following context changes than glutamatergic cells |
| url | https://doi.org/10.1038/s41598-022-13799-6 |
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