Development of nuclear DNA probes for the typing of Trypanososma cruzi
We have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Ou...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1991-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761991000300005 |
| _version_ | 1797757132022480896 |
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| author | A. Mayumi Takeuchi Yara M. Traub-Cseko |
| author_facet | A. Mayumi Takeuchi Yara M. Traub-Cseko |
| author_sort | A. Mayumi Takeuchi |
| collection | DOAJ |
| description | We have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level. |
| first_indexed | 2024-03-12T18:11:34Z |
| format | Article |
| id | doaj.art-abb1da399c0c45b3b433a9cb767d9a7a |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:11:34Z |
| publishDate | 1991-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-abb1da399c0c45b3b433a9cb767d9a7a2023-08-02T09:15:28ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601991-09-0186330731510.1590/S0074-02761991000300005Development of nuclear DNA probes for the typing of Trypanososma cruziA. Mayumi TakeuchiYara M. Traub-CsekoWe have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761991000300005Trypanosoma cruzirandom probestypingstrainsnumerical taxonomy |
| spellingShingle | A. Mayumi Takeuchi Yara M. Traub-Cseko Development of nuclear DNA probes for the typing of Trypanososma cruzi Memorias do Instituto Oswaldo Cruz Trypanosoma cruzi random probes typing strains numerical taxonomy |
| title | Development of nuclear DNA probes for the typing of Trypanososma cruzi |
| title_full | Development of nuclear DNA probes for the typing of Trypanososma cruzi |
| title_fullStr | Development of nuclear DNA probes for the typing of Trypanososma cruzi |
| title_full_unstemmed | Development of nuclear DNA probes for the typing of Trypanososma cruzi |
| title_short | Development of nuclear DNA probes for the typing of Trypanososma cruzi |
| title_sort | development of nuclear dna probes for the typing of trypanososma cruzi |
| topic | Trypanosoma cruzi random probes typing strains numerical taxonomy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761991000300005 |
| work_keys_str_mv | AT amayumitakeuchi developmentofnucleardnaprobesforthetypingoftrypanososmacruzi AT yaramtraubcseko developmentofnucleardnaprobesforthetypingoftrypanososmacruzi |