Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium <i>Halomonas</i> sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance
Salt stress is an important adverse condition encountered during plant and microbe growth in terrestrial soil ecosystems. Currently, how ice plant (<i>Mesembryanthemum crystallinum</i>) growth-promoting endophytic bacteria (EB) cope with salt stress and regulate growth and the genes resp...
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MDPI AG
2020-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/1/88 |
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| author | Jian Zhang Pengcheng Wang Hongmei Tian Zhen Tao Tingting Guo |
| author_facet | Jian Zhang Pengcheng Wang Hongmei Tian Zhen Tao Tingting Guo |
| author_sort | Jian Zhang |
| collection | DOAJ |
| description | Salt stress is an important adverse condition encountered during plant and microbe growth in terrestrial soil ecosystems. Currently, how ice plant (<i>Mesembryanthemum crystallinum</i>) growth-promoting endophytic bacteria (EB) cope with salt stress and regulate growth and the genes responsible for salt tolerance remain unknown. We applied RNA-Seq technology to determine the growth mechanism of the EB <i>Halomonas</i> sp. MC1 strain and the genes involved in salt tolerance. A total of 893 genes were significantly regulated after salt treatment. These genes included 401 upregulated and 492 downregulated genes. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes analysis revealed that the most enriched genes included those related to the outer membrane-bounded periplasmic space, ATPase activity, catabolic process, and proton transmembrane transport. The quantitative real-time polymerase chain reaction data were similar to those obtained from RNA-Seq. The MC1 strain maintained survival under salt stress by regulating cellular and metabolic processes and pyruvate metabolism pathways such as organic and carboxylic acid catabolic pathways. We highlighted the response mechanism of <i>Halomonas</i> sp. MC1 to fully understand the dynamics of complex salt−microbe interactions. |
| first_indexed | 2024-04-13T14:30:52Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-13T14:30:52Z |
| publishDate | 2020-01-01 |
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| series | Microorganisms |
| spelling | doaj.art-abb28044ba084f41a81862305f3e9b962022-12-22T02:43:11ZengMDPI AGMicroorganisms2076-26072020-01-01818810.3390/microorganisms8010088microorganisms8010088Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium <i>Halomonas</i> sp. Strain MC1 to Identify the Genes Involved in Salt ToleranceJian Zhang0Pengcheng Wang1Hongmei Tian2Zhen Tao3Tingting Guo4Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui, ChinaSalt stress is an important adverse condition encountered during plant and microbe growth in terrestrial soil ecosystems. Currently, how ice plant (<i>Mesembryanthemum crystallinum</i>) growth-promoting endophytic bacteria (EB) cope with salt stress and regulate growth and the genes responsible for salt tolerance remain unknown. We applied RNA-Seq technology to determine the growth mechanism of the EB <i>Halomonas</i> sp. MC1 strain and the genes involved in salt tolerance. A total of 893 genes were significantly regulated after salt treatment. These genes included 401 upregulated and 492 downregulated genes. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes analysis revealed that the most enriched genes included those related to the outer membrane-bounded periplasmic space, ATPase activity, catabolic process, and proton transmembrane transport. The quantitative real-time polymerase chain reaction data were similar to those obtained from RNA-Seq. The MC1 strain maintained survival under salt stress by regulating cellular and metabolic processes and pyruvate metabolism pathways such as organic and carboxylic acid catabolic pathways. We highlighted the response mechanism of <i>Halomonas</i> sp. MC1 to fully understand the dynamics of complex salt−microbe interactions.https://www.mdpi.com/2076-2607/8/1/88bacterial growthendophytes<i>halomonas</i> sp.salt stresstranscriptome analysis |
| spellingShingle | Jian Zhang Pengcheng Wang Hongmei Tian Zhen Tao Tingting Guo Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium <i>Halomonas</i> sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance Microorganisms bacterial growth endophytes <i>halomonas</i> sp. salt stress transcriptome analysis |
| title | Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium <i>Halomonas</i> sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance |
| title_full | Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium <i>Halomonas</i> sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance |
| title_fullStr | Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium <i>Halomonas</i> sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance |
| title_full_unstemmed | Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium <i>Halomonas</i> sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance |
| title_short | Transcriptome Analysis of Ice Plant Growth-Promoting Endophytic Bacterium <i>Halomonas</i> sp. Strain MC1 to Identify the Genes Involved in Salt Tolerance |
| title_sort | transcriptome analysis of ice plant growth promoting endophytic bacterium i halomonas i sp strain mc1 to identify the genes involved in salt tolerance |
| topic | bacterial growth endophytes <i>halomonas</i> sp. salt stress transcriptome analysis |
| url | https://www.mdpi.com/2076-2607/8/1/88 |
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