Spontaneous mouse lymphoma in patient-derived tumor xenografts: The importance of systematic analysis of xenografted human tumor tissues in preclinical efficacy trials
Patient-derived tumor xenograft (PDX) is now largely recognized as a key preclinical model for cancer research, mimicking patient tumor phenotype and genotype. Immunodeficient mice, well-known to develop spontaneous lymphoma, are required for PDX growth. As for all animal models used for further cli...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | Translational Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S193652332100125X |
| _version_ | 1818644777596354560 |
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| author | Sophie Chateau-Joubert Miriam Hopfe Sophie Richon Didier Decaudin Sergio Roman-Roman Edouard Reyes-Gomez Ivan Bieche Fariba Nemati Virginie Dangles-Marie |
| author_facet | Sophie Chateau-Joubert Miriam Hopfe Sophie Richon Didier Decaudin Sergio Roman-Roman Edouard Reyes-Gomez Ivan Bieche Fariba Nemati Virginie Dangles-Marie |
| author_sort | Sophie Chateau-Joubert |
| collection | DOAJ |
| description | Patient-derived tumor xenograft (PDX) is now largely recognized as a key preclinical model for cancer research, mimicking patient tumor phenotype and genotype. Immunodeficient mice, well-known to develop spontaneous lymphoma, are required for PDX growth. As for all animal models used for further clinical translation, a robust experimental design is strongly required to lead to conclusive results. Here we briefly report unintentional co-engraftment of mouse lymphoma during expansion of well-established PDXs to illustrate the importance of systematic check of the PDX identity to avoid misinterpretation. Besides, this quality control based on complementary approaches deserves a more detailed description in materials and methods section to ensure experimental validity and reproducibility. |
| first_indexed | 2024-12-17T00:20:15Z |
| format | Article |
| id | doaj.art-abb2d6a5a8f546d48fb4d2706bc60346 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-12-17T00:20:15Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-abb2d6a5a8f546d48fb4d2706bc603462022-12-21T22:10:35ZengElsevierTranslational Oncology1936-52332021-08-01148101133Spontaneous mouse lymphoma in patient-derived tumor xenografts: The importance of systematic analysis of xenografted human tumor tissues in preclinical efficacy trialsSophie Chateau-Joubert0Miriam Hopfe1Sophie Richon2Didier Decaudin3Sergio Roman-Roman4Edouard Reyes-Gomez5Ivan Bieche6Fariba Nemati7Virginie Dangles-Marie8Unité d'Histologie et d'Anatomie Pathologique, Ecole Nationale Vétérinaire d'Alfort, 94704 Maisons-Alfort, France; Laboratoire d'anatomo-cytopathologie, BioPôle Alfort, Ecole Nationale Vétérinaire d'Alfort, 94704 Maisons-Alfort, FranceBiologics Testing Solutions, Charles River Biopharmaceutical Services GmbH, Max-Planck-Str. 15A, 40699 Erkrath, GermanyLaboratory of preclinical investigation, Translational Research Department, Institut Curie, PSL Research University, 75005 Paris, France; UMR 144, Institut Curie, PSL Research University, CNRS, Paris, FranceLaboratory of preclinical investigation, Translational Research Department, Institut Curie, PSL Research University, 75005 Paris, France; Department of Medical Oncology, Institut Curie, 75005 Paris, FranceLaboratory of preclinical investigation, Translational Research Department, Institut Curie, PSL Research University, 75005 Paris, France; Translational Research Department, Institut Curie, PSL Research University, 75005 Paris, FranceUnité d'Histologie et d'Anatomie Pathologique, Ecole Nationale Vétérinaire d'Alfort, 94704 Maisons-Alfort, France; Laboratoire d'anatomo-cytopathologie, BioPôle Alfort, Ecole Nationale Vétérinaire d'Alfort, 94704 Maisons-Alfort, France; U955 - IMRB, Inserm, Ecole Nationale Vétérinaire d'Alfort, UPEC, Maisons-Alfort F-94700, FranceDepartment of Genetics, Pharmacogenomics Unit, Institut Curie, PSL Research University, Paris, France; Faculty of Pharmacy, Université Paris Descartes, Paris, FranceLaboratory of preclinical investigation, Translational Research Department, Institut Curie, PSL Research University, 75005 Paris, FranceLaboratory of preclinical investigation, Translational Research Department, Institut Curie, PSL Research University, 75005 Paris, France; Faculty of Pharmacy, Université Paris Descartes, Paris, France; In vivo Experiment Platform, PSL Research University, 75005 Paris, France; Corresponding author at: Research Center, Institut Curie, 12 rue Lhomond, F-75005 Paris, France.Patient-derived tumor xenograft (PDX) is now largely recognized as a key preclinical model for cancer research, mimicking patient tumor phenotype and genotype. Immunodeficient mice, well-known to develop spontaneous lymphoma, are required for PDX growth. As for all animal models used for further clinical translation, a robust experimental design is strongly required to lead to conclusive results. Here we briefly report unintentional co-engraftment of mouse lymphoma during expansion of well-established PDXs to illustrate the importance of systematic check of the PDX identity to avoid misinterpretation. Besides, this quality control based on complementary approaches deserves a more detailed description in materials and methods section to ensure experimental validity and reproducibility.http://www.sciencedirect.com/science/article/pii/S193652332100125XPatient-derived tumor xenograftImmunodeficient miceSpontaneous mouse lymphomaExperimental biasReproducibility of experimental results |
| spellingShingle | Sophie Chateau-Joubert Miriam Hopfe Sophie Richon Didier Decaudin Sergio Roman-Roman Edouard Reyes-Gomez Ivan Bieche Fariba Nemati Virginie Dangles-Marie Spontaneous mouse lymphoma in patient-derived tumor xenografts: The importance of systematic analysis of xenografted human tumor tissues in preclinical efficacy trials Translational Oncology Patient-derived tumor xenograft Immunodeficient mice Spontaneous mouse lymphoma Experimental bias Reproducibility of experimental results |
| title | Spontaneous mouse lymphoma in patient-derived tumor xenografts: The importance of systematic analysis of xenografted human tumor tissues in preclinical efficacy trials |
| title_full | Spontaneous mouse lymphoma in patient-derived tumor xenografts: The importance of systematic analysis of xenografted human tumor tissues in preclinical efficacy trials |
| title_fullStr | Spontaneous mouse lymphoma in patient-derived tumor xenografts: The importance of systematic analysis of xenografted human tumor tissues in preclinical efficacy trials |
| title_full_unstemmed | Spontaneous mouse lymphoma in patient-derived tumor xenografts: The importance of systematic analysis of xenografted human tumor tissues in preclinical efficacy trials |
| title_short | Spontaneous mouse lymphoma in patient-derived tumor xenografts: The importance of systematic analysis of xenografted human tumor tissues in preclinical efficacy trials |
| title_sort | spontaneous mouse lymphoma in patient derived tumor xenografts the importance of systematic analysis of xenografted human tumor tissues in preclinical efficacy trials |
| topic | Patient-derived tumor xenograft Immunodeficient mice Spontaneous mouse lymphoma Experimental bias Reproducibility of experimental results |
| url | http://www.sciencedirect.com/science/article/pii/S193652332100125X |
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