The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle
Abstract The endometrial microbiota composition may be associated with implantation success. However, a ‘core’ composition has not yet been defined. This exploratory study analysed the endometrial microbiota by 16S rRNA sequencing (V1–V2 region) of 141 infertile women whose first IVF/ICSI cycle fail...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-03-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-30591-2 |
| _version_ | 1797864649872375808 |
|---|---|
| author | Bich Ngoc Bui Nienke van Hoogenhuijze Marco Viveen Femke Mol Gijs Teklenburg Jan-Peter de Bruin Dagmar Besselink Linda Stevens Brentjens Shari Mackens Malbert R. C. Rogers Gaby S. Steba Frank Broekmans Fernanda L. Paganelli Janneke H. H. M. van de Wijgert |
| author_facet | Bich Ngoc Bui Nienke van Hoogenhuijze Marco Viveen Femke Mol Gijs Teklenburg Jan-Peter de Bruin Dagmar Besselink Linda Stevens Brentjens Shari Mackens Malbert R. C. Rogers Gaby S. Steba Frank Broekmans Fernanda L. Paganelli Janneke H. H. M. van de Wijgert |
| author_sort | Bich Ngoc Bui |
| collection | DOAJ |
| description | Abstract The endometrial microbiota composition may be associated with implantation success. However, a ‘core’ composition has not yet been defined. This exploratory study analysed the endometrial microbiota by 16S rRNA sequencing (V1–V2 region) of 141 infertile women whose first IVF/ICSI cycle failed and compared the microbiota profiles of women with and without a live birth within 12 months of follow-up, and by infertility cause and type. Lactobacillus was the most abundant genus in the majority of samples. Women with a live birth compared to those without had significantly higher Lactobacillus crispatus relative abundance (RA) (p = 0.029), and a smaller proportion of them had ≤ 10% L. crispatus RA (42.1% and 70.4%, respectively; p = 0.015). A smaller proportion of women in the male factor infertility group had ≤ 10% L. crispatus RA compared to women in the unexplained and other infertility causes groups combined (p = 0.030). Women with primary infertility compared to secondary infertility had significantly higher L. crispatus RA (p = 0.004); lower proportions of them had ≤ 10% L. crispatus RA (p = 0.009) and > 10% Gardnerella vaginalis RA (p = 0.019). In conclusion, IVF/ICSI success may be associated with L. crispatus RA and secondary infertility with endometrial dysbiosis, more often than primary infertility. These hypotheses should be tested in rigorous well-powered longitudinal studies. |
| first_indexed | 2024-04-09T22:55:20Z |
| format | Article |
| id | doaj.art-abb9eaa3cdac4345be16bfcea9358e5b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:55:20Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-abb9eaa3cdac4345be16bfcea9358e5b2023-03-22T11:18:12ZengNature PortfolioScientific Reports2045-23222023-03-0113111110.1038/s41598-023-30591-2The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycleBich Ngoc Bui0Nienke van Hoogenhuijze1Marco Viveen2Femke Mol3Gijs Teklenburg4Jan-Peter de Bruin5Dagmar Besselink6Linda Stevens Brentjens7Shari Mackens8Malbert R. C. Rogers9Gaby S. Steba10Frank Broekmans11Fernanda L. Paganelli12Janneke H. H. M. van de Wijgert13Department of Gynaecology and Reproductive Medicine, University Medical Centre UtrechtDepartment of Gynaecology and Reproductive Medicine, University Medical Centre UtrechtDepartment of Medical Microbiology, University Medical Centre UtrechtCenter for Reproductive Medicine, Reproduction and Development, Amsterdam University Medical Centre, University of AmsterdamIsala Fertility Clinic, Isala HospitalDepartment of Obstetrics and Gynaecology, Jeroen Bosch HospitalDepartment of Obstetrics and Gynaecology, Radboud University Medical CentreDepartment of Obstetrics and Gynaecology, Maastricht University Medical CentreBrussels IVF, Universitair Ziekenhuis Brussel, Vrije Universiteit BrusselDepartment of Medical Microbiology, University Medical Centre UtrechtDepartment of Gynaecology and Reproductive Medicine, University Medical Centre UtrechtDepartment of Gynaecology and Reproductive Medicine, University Medical Centre UtrechtDepartment of Medical Microbiology, University Medical Centre UtrechtJulius Center for Health Sciences and Primary Care, Utrecht UniversityAbstract The endometrial microbiota composition may be associated with implantation success. However, a ‘core’ composition has not yet been defined. This exploratory study analysed the endometrial microbiota by 16S rRNA sequencing (V1–V2 region) of 141 infertile women whose first IVF/ICSI cycle failed and compared the microbiota profiles of women with and without a live birth within 12 months of follow-up, and by infertility cause and type. Lactobacillus was the most abundant genus in the majority of samples. Women with a live birth compared to those without had significantly higher Lactobacillus crispatus relative abundance (RA) (p = 0.029), and a smaller proportion of them had ≤ 10% L. crispatus RA (42.1% and 70.4%, respectively; p = 0.015). A smaller proportion of women in the male factor infertility group had ≤ 10% L. crispatus RA compared to women in the unexplained and other infertility causes groups combined (p = 0.030). Women with primary infertility compared to secondary infertility had significantly higher L. crispatus RA (p = 0.004); lower proportions of them had ≤ 10% L. crispatus RA (p = 0.009) and > 10% Gardnerella vaginalis RA (p = 0.019). In conclusion, IVF/ICSI success may be associated with L. crispatus RA and secondary infertility with endometrial dysbiosis, more often than primary infertility. These hypotheses should be tested in rigorous well-powered longitudinal studies.https://doi.org/10.1038/s41598-023-30591-2 |
| spellingShingle | Bich Ngoc Bui Nienke van Hoogenhuijze Marco Viveen Femke Mol Gijs Teklenburg Jan-Peter de Bruin Dagmar Besselink Linda Stevens Brentjens Shari Mackens Malbert R. C. Rogers Gaby S. Steba Frank Broekmans Fernanda L. Paganelli Janneke H. H. M. van de Wijgert The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle Scientific Reports |
| title | The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle |
| title_full | The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle |
| title_fullStr | The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle |
| title_full_unstemmed | The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle |
| title_short | The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle |
| title_sort | endometrial microbiota of women with or without a live birth within 12 months after a first failed ivf icsi cycle |
| url | https://doi.org/10.1038/s41598-023-30591-2 |
| work_keys_str_mv | AT bichngocbui theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT nienkevanhoogenhuijze theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT marcoviveen theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT femkemol theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT gijsteklenburg theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT janpeterdebruin theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT dagmarbesselink theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT lindastevensbrentjens theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT sharimackens theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT malbertrcrogers theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT gabyssteba theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT frankbroekmans theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT fernandalpaganelli theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT jannekehhmvandewijgert theendometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT bichngocbui endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT nienkevanhoogenhuijze endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT marcoviveen endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT femkemol endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT gijsteklenburg endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT janpeterdebruin endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT dagmarbesselink endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT lindastevensbrentjens endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT sharimackens endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT malbertrcrogers endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT gabyssteba endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT frankbroekmans endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT fernandalpaganelli endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle AT jannekehhmvandewijgert endometrialmicrobiotaofwomenwithorwithoutalivebirthwithin12monthsafterafirstfailedivficsicycle |