Correlation of Metabolic Profiles of Plasma and Cerebrospinal Fluid of High-Grade Glioma Patients
This work compares the metabolic profiles of plasma and the cerebrospinal fluid (CSF) of the patients with high-grade (III and IV) gliomas and the conditionally healthy controls using the wide-range targeted screening of low molecular metabolites by HPLC-MS/MS. The obtained data were analyzed using...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/11/3/133 |
| _version_ | 1797395252610334720 |
|---|---|
| author | Artem D. Rogachev Nikolay A. Alemasov Vladimir A. Ivanisenko Nikita V. Ivanisenko Evgeniy V. Gaisler Olga S. Oleshko Sergey V. Cheresiz Sergey V. Mishinov Vyacheslav V. Stupak Andrey G. Pokrovsky |
| author_facet | Artem D. Rogachev Nikolay A. Alemasov Vladimir A. Ivanisenko Nikita V. Ivanisenko Evgeniy V. Gaisler Olga S. Oleshko Sergey V. Cheresiz Sergey V. Mishinov Vyacheslav V. Stupak Andrey G. Pokrovsky |
| author_sort | Artem D. Rogachev |
| collection | DOAJ |
| description | This work compares the metabolic profiles of plasma and the cerebrospinal fluid (CSF) of the patients with high-grade (III and IV) gliomas and the conditionally healthy controls using the wide-range targeted screening of low molecular metabolites by HPLC-MS/MS. The obtained data were analyzed using robust linear regression with Huber’s M-estimates, and a number of metabolites with correlated content in plasma and CSF was identified. The statistical analysis shows a significant correlation of metabolite content in plasma and CSF samples for the majority of metabolites. Several metabolites were shown to have high correlation in the control samples, but not in the glioma patients. This can be due to the specific metabolic processes in the glioma patients or to the damaged integrity of blood-brain barrier. The results of our study may be useful for the understanding of molecular mechanisms underlying the development of gliomas, as well as for the search of potential biomarkers for the minimally invasive diagnostic procedures of gliomas. |
| first_indexed | 2024-03-09T00:32:45Z |
| format | Article |
| id | doaj.art-abbac36601274e459373ecc5a1105608 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T00:32:45Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-abbac36601274e459373ecc5a11056082023-12-11T18:26:38ZengMDPI AGMetabolites2218-19892021-02-0111313310.3390/metabo11030133Correlation of Metabolic Profiles of Plasma and Cerebrospinal Fluid of High-Grade Glioma PatientsArtem D. Rogachev0Nikolay A. Alemasov1Vladimir A. Ivanisenko2Nikita V. Ivanisenko3Evgeniy V. Gaisler4Olga S. Oleshko5Sergey V. Cheresiz6Sergey V. Mishinov7Vyacheslav V. Stupak8Andrey G. Pokrovsky9V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, Pirogov str., 2, 630090 Novosibirsk, RussiaInstitute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, acad. Lavrentiev ave., 10, 630090 Novosibirsk, RussiaInstitute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, acad. Lavrentiev ave., 10, 630090 Novosibirsk, RussiaInstitute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, acad. Lavrentiev ave., 10, 630090 Novosibirsk, RussiaV. Zelman Institute for Medicine and Psychology, Novosibirsk State University, Pirogov str., 2, 630090 Novosibirsk, RussiaV. Zelman Institute for Medicine and Psychology, Novosibirsk State University, Pirogov str., 2, 630090 Novosibirsk, RussiaV. Zelman Institute for Medicine and Psychology, Novosibirsk State University, Pirogov str., 2, 630090 Novosibirsk, RussiaFSBI “Novosibirsk Research Institute of Traumatology and Orthopedics Named after Ya. L. Tsiviyan”, Frunze str., 17, 630091 Novosibirsk, RussiaFSBI “Novosibirsk Research Institute of Traumatology and Orthopedics Named after Ya. L. Tsiviyan”, Frunze str., 17, 630091 Novosibirsk, RussiaV. Zelman Institute for Medicine and Psychology, Novosibirsk State University, Pirogov str., 2, 630090 Novosibirsk, RussiaThis work compares the metabolic profiles of plasma and the cerebrospinal fluid (CSF) of the patients with high-grade (III and IV) gliomas and the conditionally healthy controls using the wide-range targeted screening of low molecular metabolites by HPLC-MS/MS. The obtained data were analyzed using robust linear regression with Huber’s M-estimates, and a number of metabolites with correlated content in plasma and CSF was identified. The statistical analysis shows a significant correlation of metabolite content in plasma and CSF samples for the majority of metabolites. Several metabolites were shown to have high correlation in the control samples, but not in the glioma patients. This can be due to the specific metabolic processes in the glioma patients or to the damaged integrity of blood-brain barrier. The results of our study may be useful for the understanding of molecular mechanisms underlying the development of gliomas, as well as for the search of potential biomarkers for the minimally invasive diagnostic procedures of gliomas.https://www.mdpi.com/2218-1989/11/3/133gliomametabolomicsliquid chromatographytandem mass spectrometryplasmacerebrospinal fluid |
| spellingShingle | Artem D. Rogachev Nikolay A. Alemasov Vladimir A. Ivanisenko Nikita V. Ivanisenko Evgeniy V. Gaisler Olga S. Oleshko Sergey V. Cheresiz Sergey V. Mishinov Vyacheslav V. Stupak Andrey G. Pokrovsky Correlation of Metabolic Profiles of Plasma and Cerebrospinal Fluid of High-Grade Glioma Patients Metabolites glioma metabolomics liquid chromatography tandem mass spectrometry plasma cerebrospinal fluid |
| title | Correlation of Metabolic Profiles of Plasma and Cerebrospinal Fluid of High-Grade Glioma Patients |
| title_full | Correlation of Metabolic Profiles of Plasma and Cerebrospinal Fluid of High-Grade Glioma Patients |
| title_fullStr | Correlation of Metabolic Profiles of Plasma and Cerebrospinal Fluid of High-Grade Glioma Patients |
| title_full_unstemmed | Correlation of Metabolic Profiles of Plasma and Cerebrospinal Fluid of High-Grade Glioma Patients |
| title_short | Correlation of Metabolic Profiles of Plasma and Cerebrospinal Fluid of High-Grade Glioma Patients |
| title_sort | correlation of metabolic profiles of plasma and cerebrospinal fluid of high grade glioma patients |
| topic | glioma metabolomics liquid chromatography tandem mass spectrometry plasma cerebrospinal fluid |
| url | https://www.mdpi.com/2218-1989/11/3/133 |
| work_keys_str_mv | AT artemdrogachev correlationofmetabolicprofilesofplasmaandcerebrospinalfluidofhighgradegliomapatients AT nikolayaalemasov correlationofmetabolicprofilesofplasmaandcerebrospinalfluidofhighgradegliomapatients AT vladimiraivanisenko correlationofmetabolicprofilesofplasmaandcerebrospinalfluidofhighgradegliomapatients AT nikitavivanisenko correlationofmetabolicprofilesofplasmaandcerebrospinalfluidofhighgradegliomapatients AT evgeniyvgaisler correlationofmetabolicprofilesofplasmaandcerebrospinalfluidofhighgradegliomapatients AT olgasoleshko correlationofmetabolicprofilesofplasmaandcerebrospinalfluidofhighgradegliomapatients AT sergeyvcheresiz correlationofmetabolicprofilesofplasmaandcerebrospinalfluidofhighgradegliomapatients AT sergeyvmishinov correlationofmetabolicprofilesofplasmaandcerebrospinalfluidofhighgradegliomapatients AT vyacheslavvstupak correlationofmetabolicprofilesofplasmaandcerebrospinalfluidofhighgradegliomapatients AT andreygpokrovsky correlationofmetabolicprofilesofplasmaandcerebrospinalfluidofhighgradegliomapatients |