Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men
In 2021, approximately 248,530 new prostate cancer (PCa) cases are estimated in the United States. Hispanic/Latinos (H/L) are the second largest racial/ethnic group in the US. The objective of this study was to assess DNA methylation patterns between aggressive and indolent PCa along with ancestry p...
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MDPI AG
2021-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/1/2 |
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| author | Anders Berglund Jaime Matta Jarline Encarnación-Medina Carmen Ortiz-Sanchéz Julie Dutil Raymond Linares Joshua Marcial Caren Abreu-Takemura Natasha Moreno Ryan Putney Ratna Chakrabarti Hui-Yi Lin Kosj Yamoah Carlos Diaz Osterman Liang Wang Jasreman Dhillon Youngchul Kim Seung Joon Kim Gilberto Ruiz-Deya Jong Y. Park |
| author_facet | Anders Berglund Jaime Matta Jarline Encarnación-Medina Carmen Ortiz-Sanchéz Julie Dutil Raymond Linares Joshua Marcial Caren Abreu-Takemura Natasha Moreno Ryan Putney Ratna Chakrabarti Hui-Yi Lin Kosj Yamoah Carlos Diaz Osterman Liang Wang Jasreman Dhillon Youngchul Kim Seung Joon Kim Gilberto Ruiz-Deya Jong Y. Park |
| author_sort | Anders Berglund |
| collection | DOAJ |
| description | In 2021, approximately 248,530 new prostate cancer (PCa) cases are estimated in the United States. Hispanic/Latinos (H/L) are the second largest racial/ethnic group in the US. The objective of this study was to assess DNA methylation patterns between aggressive and indolent PCa along with ancestry proportions in 49 H/L men from Puerto Rico (PR). Prostate tumors were classified as aggressive (<i>n</i> = 17) and indolent (<i>n</i> = 32) based on the Gleason score. Genomic DNA samples were extracted by macro-dissection. DNA methylation patterns were assessed using the Illumina EPIC DNA methylation platform. We used ADMIXTURE to estimate global ancestry proportions. We identified 892 differentially methylated genes in prostate tumor tissues as compared with normal tissues. Based on an epigenetic clock model, we observed that the total number of stem cell divisions (TNSC) and stem cell division rate (SCDR) were significantly higher in tumor than adjacent normal tissues. Regarding PCa aggressiveness, 141 differentially methylated genes were identified. Ancestry proportions of PR men were estimated as African, European, and Indigenous American; these were 24.1%, 64.2%, and 11.7%, respectively. The identification of DNA methylation profiles associated with risk and aggressiveness of PCa in PR H/L men will shed light on potential mechanisms contributing to PCa disparities in PR population. |
| first_indexed | 2024-03-10T01:51:15Z |
| format | Article |
| id | doaj.art-abbef4b79dbc4d7d960fb890923db350 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T01:51:15Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-abbef4b79dbc4d7d960fb890923db3502023-11-23T13:05:30ZengMDPI AGBiomolecules2218-273X2021-12-01121210.3390/biom12010002Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican MenAnders Berglund0Jaime Matta1Jarline Encarnación-Medina2Carmen Ortiz-Sanchéz3Julie Dutil4Raymond Linares5Joshua Marcial6Caren Abreu-Takemura7Natasha Moreno8Ryan Putney9Ratna Chakrabarti10Hui-Yi Lin11Kosj Yamoah12Carlos Diaz Osterman13Liang Wang14Jasreman Dhillon15Youngchul Kim16Seung Joon Kim17Gilberto Ruiz-Deya18Jong Y. Park19Department of Biostatistics and Bioinformatics, H. Lee Moffitt Cancer Center, Tampa, FL 33612, USADepartment of Basic Sciences, Ponce Research Institute, School of Medicine, Ponce Health Sciences University, Ponce 00716-2347, Puerto RicoDepartment of Basic Sciences, Ponce Research Institute, School of Medicine, Ponce Health Sciences University, Ponce 00716-2347, Puerto RicoDepartment of Basic Sciences, Ponce Research Institute, School of Medicine, Ponce Health Sciences University, Ponce 00716-2347, Puerto RicoDepartment of Basic Sciences, Ponce Research Institute, School of Medicine, Ponce Health Sciences University, Ponce 00716-2347, Puerto RicoDepartment of Basic Sciences, Ponce Research Institute, School of Medicine, Ponce Health Sciences University, Ponce 00716-2347, Puerto RicoDepartment of Basic Sciences, Ponce Research Institute, School of Medicine, Ponce Health Sciences University, Ponce 00716-2347, Puerto RicoDepartment of Basic Sciences, Ponce Research Institute, School of Medicine, Ponce Health Sciences University, Ponce 00716-2347, Puerto RicoDepartment of Basic Sciences, Ponce Research Institute, School of Medicine, Ponce Health Sciences University, Ponce 00716-2347, Puerto RicoDepartment of Biostatistics and Bioinformatics, H. Lee Moffitt Cancer Center, Tampa, FL 33612, USABurnett School of Biomedical Sciences, University of Central Florida, Orlando, FL 32816, USABiostatistics Program, School of Public Health, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USADepartment of Radiation Oncology, H. Lee Moffitt Cancer Center, Tampa, FL 33612, USADepartment of Basic Sciences, Ponce Research Institute, School of Medicine, Ponce Health Sciences University, Ponce 00716-2347, Puerto RicoDepartment of Molecular Biology, H. Lee Moffitt Cancer Center, Tampa, FL 33612, USADepartment of Pathology, H. Lee Moffitt Cancer Center, Tampa, FL 33612, USADepartment of Biostatistics and Bioinformatics, H. Lee Moffitt Cancer Center, Tampa, FL 33612, USADepartment of Internal Medicine, Catholic University of Korea, Seoul 06591, KoreaDepartment of Basic Sciences, Ponce Research Institute, School of Medicine, Ponce Health Sciences University, Ponce 00716-2347, Puerto RicoDepartment of Cancer Epidemiology, H. Lee Moffitt Cancer Center, Tampa, FL 33612, USAIn 2021, approximately 248,530 new prostate cancer (PCa) cases are estimated in the United States. Hispanic/Latinos (H/L) are the second largest racial/ethnic group in the US. The objective of this study was to assess DNA methylation patterns between aggressive and indolent PCa along with ancestry proportions in 49 H/L men from Puerto Rico (PR). Prostate tumors were classified as aggressive (<i>n</i> = 17) and indolent (<i>n</i> = 32) based on the Gleason score. Genomic DNA samples were extracted by macro-dissection. DNA methylation patterns were assessed using the Illumina EPIC DNA methylation platform. We used ADMIXTURE to estimate global ancestry proportions. We identified 892 differentially methylated genes in prostate tumor tissues as compared with normal tissues. Based on an epigenetic clock model, we observed that the total number of stem cell divisions (TNSC) and stem cell division rate (SCDR) were significantly higher in tumor than adjacent normal tissues. Regarding PCa aggressiveness, 141 differentially methylated genes were identified. Ancestry proportions of PR men were estimated as African, European, and Indigenous American; these were 24.1%, 64.2%, and 11.7%, respectively. The identification of DNA methylation profiles associated with risk and aggressiveness of PCa in PR H/L men will shed light on potential mechanisms contributing to PCa disparities in PR population.https://www.mdpi.com/2218-273X/12/1/2prostate cancerDNA methylationaggressivenessHispanic/Latino populationancestry structure |
| spellingShingle | Anders Berglund Jaime Matta Jarline Encarnación-Medina Carmen Ortiz-Sanchéz Julie Dutil Raymond Linares Joshua Marcial Caren Abreu-Takemura Natasha Moreno Ryan Putney Ratna Chakrabarti Hui-Yi Lin Kosj Yamoah Carlos Diaz Osterman Liang Wang Jasreman Dhillon Youngchul Kim Seung Joon Kim Gilberto Ruiz-Deya Jong Y. Park Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men Biomolecules prostate cancer DNA methylation aggressiveness Hispanic/Latino population ancestry structure |
| title | Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men |
| title_full | Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men |
| title_fullStr | Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men |
| title_full_unstemmed | Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men |
| title_short | Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men |
| title_sort | dysregulation of dna methylation and epigenetic clocks in prostate cancer among puerto rican men |
| topic | prostate cancer DNA methylation aggressiveness Hispanic/Latino population ancestry structure |
| url | https://www.mdpi.com/2218-273X/12/1/2 |
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