Biophysical Properties and Antiviral Activities of Measles Fusion Protein Derived Peptide Conjugated with 25-Hydroxycholesterol
Measles virus (MV) infection is re-emerging, despite the availability of an effective vaccine. The mechanism of MV entry into a target cell relies on coordinated action between the MV hemagglutinin (H) receptor binding protein and the fusion envelope glycoprotein (F) which mediates fusion between th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/11/1869 |
| _version_ | 1811284447507513344 |
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| author | Bárbara Gomes Nuno C. Santos Matteo Porotto |
| author_facet | Bárbara Gomes Nuno C. Santos Matteo Porotto |
| author_sort | Bárbara Gomes |
| collection | DOAJ |
| description | Measles virus (MV) infection is re-emerging, despite the availability of an effective vaccine. The mechanism of MV entry into a target cell relies on coordinated action between the MV hemagglutinin (H) receptor binding protein and the fusion envelope glycoprotein (F) which mediates fusion between the viral and cell membranes. Peptides derived from the C-terminal heptad repeat (HRC) of F can interfere with this process, blocking MV infection. As previously described, biophysical properties of HRC-derived peptides modulate their antiviral potency. In this work, we characterized a MV peptide fusion inhibitor conjugated to 25-hydroxycholesterol (25HC), a cholesterol derivative with intrinsic antiviral activity, and evaluated its interaction with membrane model systems and human blood cells. The peptide (MV |
| first_indexed | 2024-04-13T02:28:44Z |
| format | Article |
| id | doaj.art-abbf2d34e16e43c98aa25f4e7dd669b4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T02:28:44Z |
| publishDate | 2017-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-abbf2d34e16e43c98aa25f4e7dd669b42022-12-22T03:06:41ZengMDPI AGMolecules1420-30492017-10-012211186910.3390/molecules22111869molecules22111869Biophysical Properties and Antiviral Activities of Measles Fusion Protein Derived Peptide Conjugated with 25-HydroxycholesterolBárbara Gomes0Nuno C. Santos1Matteo Porotto2Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisbon, PortugalInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisbon, PortugalCenter for Host-Pathogen Interaction, Columbia University Medical Center, 701 W. 168th St., New York, NY 10032, USAMeasles virus (MV) infection is re-emerging, despite the availability of an effective vaccine. The mechanism of MV entry into a target cell relies on coordinated action between the MV hemagglutinin (H) receptor binding protein and the fusion envelope glycoprotein (F) which mediates fusion between the viral and cell membranes. Peptides derived from the C-terminal heptad repeat (HRC) of F can interfere with this process, blocking MV infection. As previously described, biophysical properties of HRC-derived peptides modulate their antiviral potency. In this work, we characterized a MV peptide fusion inhibitor conjugated to 25-hydroxycholesterol (25HC), a cholesterol derivative with intrinsic antiviral activity, and evaluated its interaction with membrane model systems and human blood cells. The peptide (MVhttps://www.mdpi.com/1420-3049/22/11/1869measles virusfusionpeptide25-hydroxycholesterol |
| spellingShingle | Bárbara Gomes Nuno C. Santos Matteo Porotto Biophysical Properties and Antiviral Activities of Measles Fusion Protein Derived Peptide Conjugated with 25-Hydroxycholesterol Molecules measles virus fusion peptide 25-hydroxycholesterol |
| title | Biophysical Properties and Antiviral Activities of Measles Fusion Protein Derived Peptide Conjugated with 25-Hydroxycholesterol |
| title_full | Biophysical Properties and Antiviral Activities of Measles Fusion Protein Derived Peptide Conjugated with 25-Hydroxycholesterol |
| title_fullStr | Biophysical Properties and Antiviral Activities of Measles Fusion Protein Derived Peptide Conjugated with 25-Hydroxycholesterol |
| title_full_unstemmed | Biophysical Properties and Antiviral Activities of Measles Fusion Protein Derived Peptide Conjugated with 25-Hydroxycholesterol |
| title_short | Biophysical Properties and Antiviral Activities of Measles Fusion Protein Derived Peptide Conjugated with 25-Hydroxycholesterol |
| title_sort | biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25 hydroxycholesterol |
| topic | measles virus fusion peptide 25-hydroxycholesterol |
| url | https://www.mdpi.com/1420-3049/22/11/1869 |
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