Optimisation of Centrifugal Speed for PRPBAG®: A Novel Multiple Bag System for Preparing Platelet-Rich Plasma
Objective:This study aimed to identify the optimal centrifugal speed for the preparation of platelet-rich plasma (PRP) with PRPBAG®, a novel multi-bag system.Methods:The study included 120 (60 women and 60 men) clinically healthy volunteers aged 18-35 years. Participants were divided into three age-...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2021-07-01
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| Series: | Bezmiâlem Science |
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http://bezmialemscience.org/archives/archive-detail/article-preview/optimisation-of-centrifugal-speed-for-prpbagsup-su/47960
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| _version_ | 1828016838103007232 |
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| author | Güven ÇETİN Nang Hseng KYIO Seda TURGUT Tuba ÖZKAN |
| author_facet | Güven ÇETİN Nang Hseng KYIO Seda TURGUT Tuba ÖZKAN |
| author_sort | Güven ÇETİN |
| collection | DOAJ |
| description | Objective:This study aimed to identify the optimal centrifugal speed for the preparation of platelet-rich plasma (PRP) with PRPBAG®, a novel multi-bag system.Methods:The study included 120 (60 women and 60 men) clinically healthy volunteers aged 18-35 years. Participants were divided into three age-sex matched groups according to the centrifugation speeds as follows: 1600 rpm (n=40), 1800 rpm (n=40) or 2000 rpm (n=40). Whole blood (150 mL) was drawn from the volunteers, and centrifugation was performed for 10 minutes. After the first spin, the erythrocytes precipitated in the first bag, and the supernatant plasma was transferred into the second bag. Plasma centrifugation was performed at 3500 rpm for 15 minutes. The high-quality PRP product precipitated in the second bag. The supernatant platelet-poor plasma was transferred to the third bag and destroyed. Blood cell count of whole blood and PRP product was measured.Results:The mean white blood cell level of PRP in the 1800 rpm group was higher than the other groups (p=0.027). The mean platelet levels were 1005.22±105.96, 1743.7±145.2 and 1743.7±145.2 103/uL in the 1600, 1800 and 2000 rpm groups, respectively, after centrifugation (p<0.001). Accordingly, the platelet yield (fold increase) was significantly greater in the 1800 rpm group than in the other groups (p<0.001).Conclusion:This study showed that 1800 rpm for 10 minutes is the optimum first centrifugation speed for preparing PRP with PRPBAG®, as it provides an approximately eightfold increase in platelet concentration in PRPBAG®. |
| first_indexed | 2024-04-10T10:40:18Z |
| format | Article |
| id | doaj.art-abc0ee9a12c44def860bd475c09af112 |
| institution | Directory Open Access Journal |
| issn | 2148-2373 2148-2373 |
| language | English |
| last_indexed | 2024-04-10T10:40:18Z |
| publishDate | 2021-07-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Bezmiâlem Science |
| spelling | doaj.art-abc0ee9a12c44def860bd475c09af1122023-02-15T16:20:38ZengGalenos Publishing HouseBezmiâlem Science2148-23732148-23732021-07-019335135510.14235/bas.galenos.2021.584213049054Optimisation of Centrifugal Speed for PRPBAG®: A Novel Multiple Bag System for Preparing Platelet-Rich PlasmaGüven ÇETİN0Nang Hseng KYIO1Seda TURGUT2Tuba ÖZKAN3 Bezmialem Vakıf University Hospital, Clinic of Hematology, İstanbul, Turkey Bezmialem Vakıf University Hospital, Clinic of Hematology, İstanbul, Turkey University of Health Sciences Turkey, Bakırköy Dr. Sadi Konuk Training and Research Hospital, Clinic of Endocrinology, İstanbul, Turkey İstanbul University-Cerrahpaşa, Cerrahpaşa Faculty of Medicine, Clinic of Hematology, İstanbul, Turkey Objective:This study aimed to identify the optimal centrifugal speed for the preparation of platelet-rich plasma (PRP) with PRPBAG®, a novel multi-bag system.Methods:The study included 120 (60 women and 60 men) clinically healthy volunteers aged 18-35 years. Participants were divided into three age-sex matched groups according to the centrifugation speeds as follows: 1600 rpm (n=40), 1800 rpm (n=40) or 2000 rpm (n=40). Whole blood (150 mL) was drawn from the volunteers, and centrifugation was performed for 10 minutes. After the first spin, the erythrocytes precipitated in the first bag, and the supernatant plasma was transferred into the second bag. Plasma centrifugation was performed at 3500 rpm for 15 minutes. The high-quality PRP product precipitated in the second bag. The supernatant platelet-poor plasma was transferred to the third bag and destroyed. Blood cell count of whole blood and PRP product was measured.Results:The mean white blood cell level of PRP in the 1800 rpm group was higher than the other groups (p=0.027). The mean platelet levels were 1005.22±105.96, 1743.7±145.2 and 1743.7±145.2 103/uL in the 1600, 1800 and 2000 rpm groups, respectively, after centrifugation (p<0.001). Accordingly, the platelet yield (fold increase) was significantly greater in the 1800 rpm group than in the other groups (p<0.001).Conclusion:This study showed that 1800 rpm for 10 minutes is the optimum first centrifugation speed for preparing PRP with PRPBAG®, as it provides an approximately eightfold increase in platelet concentration in PRPBAG®. http://bezmialemscience.org/archives/archive-detail/article-preview/optimisation-of-centrifugal-speed-for-prpbagsup-su/47960 platelet-rich plasmaprpbagoptimisation |
| spellingShingle | Güven ÇETİN Nang Hseng KYIO Seda TURGUT Tuba ÖZKAN Optimisation of Centrifugal Speed for PRPBAG®: A Novel Multiple Bag System for Preparing Platelet-Rich Plasma Bezmiâlem Science platelet-rich plasma prpbag optimisation |
| title | Optimisation of Centrifugal Speed for PRPBAG®: A Novel Multiple Bag System for Preparing Platelet-Rich Plasma |
| title_full | Optimisation of Centrifugal Speed for PRPBAG®: A Novel Multiple Bag System for Preparing Platelet-Rich Plasma |
| title_fullStr | Optimisation of Centrifugal Speed for PRPBAG®: A Novel Multiple Bag System for Preparing Platelet-Rich Plasma |
| title_full_unstemmed | Optimisation of Centrifugal Speed for PRPBAG®: A Novel Multiple Bag System for Preparing Platelet-Rich Plasma |
| title_short | Optimisation of Centrifugal Speed for PRPBAG®: A Novel Multiple Bag System for Preparing Platelet-Rich Plasma |
| title_sort | optimisation of centrifugal speed for prpbag r a novel multiple bag system for preparing platelet rich plasma |
| topic | platelet-rich plasma prpbag optimisation |
| url |
http://bezmialemscience.org/archives/archive-detail/article-preview/optimisation-of-centrifugal-speed-for-prpbagsup-su/47960
|
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