Emergence and genomics of OXA-232-producing Klebsiella pneumoniae in a hospital in Yancheng, China
ABSTRACT: Objectives: The aims of this study were to infer the phylogenetic relationship of OXA-232-producing Klebsiella pneumoniae (OXA232Kp) strains collected from a Chinese hospital and to determine the composition and genetic background of antimicrobial resistance genes (ARGs) among these strai...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716521001466 |
| _version_ | 1819123161755549696 |
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| author | Zhichen Zhu Haifeng Huang Yumei Xu Min Wang Jinnan Lv Linlin Xu Chunlei Shi Ya Xu Ruifu Yang Liang Chen Hong Du |
| author_facet | Zhichen Zhu Haifeng Huang Yumei Xu Min Wang Jinnan Lv Linlin Xu Chunlei Shi Ya Xu Ruifu Yang Liang Chen Hong Du |
| author_sort | Zhichen Zhu |
| collection | DOAJ |
| description | ABSTRACT: Objectives: The aims of this study were to infer the phylogenetic relationship of OXA-232-producing Klebsiella pneumoniae (OXA232Kp) strains collected from a Chinese hospital and to determine the composition and genetic background of antimicrobial resistance genes (ARGs) among these strains. Methods: Three non-duplicate OXA232Kp strains were collected from a Chinese hospital. Whole-genome sequencing was used to determine their genome sequences and then a genomic comparison of ARG-carrying genetic elements from the three strains with related sequences was performed. Phylogenetic analysis was conducted by constructing a maximum-likelihood phylogenetic tree. Results: Compared with other Chinese sequence type 15 (ST15)-OXA232Kp strains, the three ST15-OXA232Kp strains in this study could be divided into a single subgroup in phylogenetic relationship. The composition and genetic background of ARGs were identical in the three strains. Three ARG-carrying genetic elements or multidrug resistance (MDR) regions were determined, including a truncated Tn2013-like IS-based transposition unit, a unit transposition Tn6867b and a 40.9-kb MDR region. Conclusion: This study reported clonal dissemination of ST15-OXA232Kp strains carrying multiple ARGs in a Chinese hospital. A comprehensive evolutionary and genomics analysis provided a deeper understanding of OXA232Kp. Further surveillance and study should be advocated to prevent the dissemination of OXA232Kp strains in China. |
| first_indexed | 2024-12-22T07:03:57Z |
| format | Article |
| id | doaj.art-abc37afbf0c5499c9db652cb8726d883 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-22T07:03:57Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-abc37afbf0c5499c9db652cb8726d8832022-12-21T18:34:43ZengElsevierJournal of Global Antimicrobial Resistance2213-71652021-09-0126194198Emergence and genomics of OXA-232-producing Klebsiella pneumoniae in a hospital in Yancheng, ChinaZhichen Zhu0Haifeng Huang1Yumei Xu2Min Wang3Jinnan Lv4Linlin Xu5Chunlei Shi6Ya Xu7Ruifu Yang8Liang Chen9Hong Du10Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR ChinaDepartment of Laboratory Medicine, The First People's Hospital of Yancheng City, Yancheng, Jiangsu, ChinaLaboratory Department, Shanghai Xuhui Central Hospital, Shanghai, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR ChinaDepartment of Laboratory Medicine, The First People's Hospital of Yancheng City, Yancheng, Jiangsu, ChinaDepartment of Laboratory Medicine, The First People's Hospital of Yancheng City, Yancheng, Jiangsu, ChinaDepartment of Laboratory Medicine, The First People's Hospital of Yancheng City, Yancheng, Jiangsu, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaHackensack-Meridian Health Center for Discovery and Innovation, Nutley, NJ 07110, USA; Hackensack Meridian School of Medicine at Seton Hall University, Nutley, NJ 07110, USADepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China; Corresponding author. Tel.: +86 512 6828 2030; fax: +86 512 6778 3581.ABSTRACT: Objectives: The aims of this study were to infer the phylogenetic relationship of OXA-232-producing Klebsiella pneumoniae (OXA232Kp) strains collected from a Chinese hospital and to determine the composition and genetic background of antimicrobial resistance genes (ARGs) among these strains. Methods: Three non-duplicate OXA232Kp strains were collected from a Chinese hospital. Whole-genome sequencing was used to determine their genome sequences and then a genomic comparison of ARG-carrying genetic elements from the three strains with related sequences was performed. Phylogenetic analysis was conducted by constructing a maximum-likelihood phylogenetic tree. Results: Compared with other Chinese sequence type 15 (ST15)-OXA232Kp strains, the three ST15-OXA232Kp strains in this study could be divided into a single subgroup in phylogenetic relationship. The composition and genetic background of ARGs were identical in the three strains. Three ARG-carrying genetic elements or multidrug resistance (MDR) regions were determined, including a truncated Tn2013-like IS-based transposition unit, a unit transposition Tn6867b and a 40.9-kb MDR region. Conclusion: This study reported clonal dissemination of ST15-OXA232Kp strains carrying multiple ARGs in a Chinese hospital. A comprehensive evolutionary and genomics analysis provided a deeper understanding of OXA232Kp. Further surveillance and study should be advocated to prevent the dissemination of OXA232Kp strains in China.http://www.sciencedirect.com/science/article/pii/S2213716521001466Klebsiella pneumoniaeOXA-232Clonal disseminationGenetic elementsST15 |
| spellingShingle | Zhichen Zhu Haifeng Huang Yumei Xu Min Wang Jinnan Lv Linlin Xu Chunlei Shi Ya Xu Ruifu Yang Liang Chen Hong Du Emergence and genomics of OXA-232-producing Klebsiella pneumoniae in a hospital in Yancheng, China Journal of Global Antimicrobial Resistance Klebsiella pneumoniae OXA-232 Clonal dissemination Genetic elements ST15 |
| title | Emergence and genomics of OXA-232-producing Klebsiella pneumoniae in a hospital in Yancheng, China |
| title_full | Emergence and genomics of OXA-232-producing Klebsiella pneumoniae in a hospital in Yancheng, China |
| title_fullStr | Emergence and genomics of OXA-232-producing Klebsiella pneumoniae in a hospital in Yancheng, China |
| title_full_unstemmed | Emergence and genomics of OXA-232-producing Klebsiella pneumoniae in a hospital in Yancheng, China |
| title_short | Emergence and genomics of OXA-232-producing Klebsiella pneumoniae in a hospital in Yancheng, China |
| title_sort | emergence and genomics of oxa 232 producing klebsiella pneumoniae in a hospital in yancheng china |
| topic | Klebsiella pneumoniae OXA-232 Clonal dissemination Genetic elements ST15 |
| url | http://www.sciencedirect.com/science/article/pii/S2213716521001466 |
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