Effect of varying amounts of linseed oil or saturated fatty acids around insemination on reproductive and blood parameters of ewes
The current study was designed to evaluate the effect of sequential low and high dietary linseed oil (LO; as omega-3 enriched fatty acid; FA) before and post insemination, respectively, on different plasma variables of ewes. Fat-tailed Qezel ewes were assigned randomly to be fed a diet enriched with...
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| Format: | Article |
| Language: | English |
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Korean Society of Animal Sciences and Technology
2023-09-01
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| Series: | Journal of Animal Science and Technology |
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| Online Access: | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e106 |
| _version_ | 1827799509179039744 |
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| author | Hamed Esmaili Mohsen Eslami Hamed Khalilvandi-Behrozyar Farhad Farrokhi-Ardabili |
| author_facet | Hamed Esmaili Mohsen Eslami Hamed Khalilvandi-Behrozyar Farhad Farrokhi-Ardabili |
| author_sort | Hamed Esmaili |
| collection | DOAJ |
| description | The current study was designed to evaluate the effect of sequential low and high dietary linseed oil (LO; as omega-3 enriched fatty acid; FA) before and post insemination, respectively, on different plasma variables of ewes. Fat-tailed Qezel ewes were assigned randomly to be fed a diet enriched with 3% LO (n = 30) or the saturated FA (SFA; n = 30) three weeks before insemination (Day 0). The lipogenic diet supplemented with 6% LO or SFA was fed after insemination until Day +21. The control ewes were fed an isocaloric and isonitrogenous diet with no additional FA during the study. Estrus was synchronized by inserting a vaginal sponge (Spongavet®) for 12 days + 500 IU equine chorionic gonadotropin (eCG; Gonaser®), and ewes were inseminated via laparoscopic approach 56–59 h after eCG injection. The size of ovarian structures was assessed by transvaginal ultrasonography at −21, −14, −2, 0, and +10 days. Blood samples were collected weekly to measure the plasma’s different biochemical variables and FA profile. Treatment did not affect the amounts of glucose, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, interleukin-10, interleukin-2, and non-esterified FA (p > 0.05). Conversely, concentrations of triglyceride, cholesterol, tumor necrosis factor-alpha, and insulin-like growth factor-1 were higher in SFA-fed ewes relative to control animals (p < 0.05). LO feeding resulted in greater amounts of n-3 FA isomers in plasma, while higher amounts of stearic acid were detected in SFA fed group 0 and +21 (p < 0.05). The number of ovarian follicles and corpora lutea also were not affected by treatment. Other reproductive variables were not affected by treatment except for the reproductive rate. It seems that LO or SFA feeding of fat-tailed ewes peri-insemination period was not superior to the isocaloric non-additional fat diet provided for the control group during the non-breeding season. |
| first_indexed | 2024-03-11T19:53:36Z |
| format | Article |
| id | doaj.art-abc3ef051c77467e99a531f726bf9901 |
| institution | Directory Open Access Journal |
| issn | 2672-0191 2055-0391 |
| language | English |
| last_indexed | 2024-03-11T19:53:36Z |
| publishDate | 2023-09-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-abc3ef051c77467e99a531f726bf99012023-10-05T01:39:24ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912023-09-0165592293810.5187/jast.2022.e106Effect of varying amounts of linseed oil or saturated fatty acids around insemination on reproductive and blood parameters of ewesHamed Esmaili0Mohsen Eslami1Hamed Khalilvandi-Behrozyar2Farhad Farrokhi-Ardabili3Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia 5756115111, IranDepartment of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia 5756115111, IranDepartment of Animal Sciences, Faculty of Agriculture, Urmia University, Urmia 5756115111, IranDepartment of Animal Sciences, Faculty of Agriculture, Urmia University, Urmia 5756115111, IranThe current study was designed to evaluate the effect of sequential low and high dietary linseed oil (LO; as omega-3 enriched fatty acid; FA) before and post insemination, respectively, on different plasma variables of ewes. Fat-tailed Qezel ewes were assigned randomly to be fed a diet enriched with 3% LO (n = 30) or the saturated FA (SFA; n = 30) three weeks before insemination (Day 0). The lipogenic diet supplemented with 6% LO or SFA was fed after insemination until Day +21. The control ewes were fed an isocaloric and isonitrogenous diet with no additional FA during the study. Estrus was synchronized by inserting a vaginal sponge (Spongavet®) for 12 days + 500 IU equine chorionic gonadotropin (eCG; Gonaser®), and ewes were inseminated via laparoscopic approach 56–59 h after eCG injection. The size of ovarian structures was assessed by transvaginal ultrasonography at −21, −14, −2, 0, and +10 days. Blood samples were collected weekly to measure the plasma’s different biochemical variables and FA profile. Treatment did not affect the amounts of glucose, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, interleukin-10, interleukin-2, and non-esterified FA (p > 0.05). Conversely, concentrations of triglyceride, cholesterol, tumor necrosis factor-alpha, and insulin-like growth factor-1 were higher in SFA-fed ewes relative to control animals (p < 0.05). LO feeding resulted in greater amounts of n-3 FA isomers in plasma, while higher amounts of stearic acid were detected in SFA fed group 0 and +21 (p < 0.05). The number of ovarian follicles and corpora lutea also were not affected by treatment. Other reproductive variables were not affected by treatment except for the reproductive rate. It seems that LO or SFA feeding of fat-tailed ewes peri-insemination period was not superior to the isocaloric non-additional fat diet provided for the control group during the non-breeding season. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e106Linseed oilSaturated fatty acid (FA)Laparoscopic inseminationEweReproduction |
| spellingShingle | Hamed Esmaili Mohsen Eslami Hamed Khalilvandi-Behrozyar Farhad Farrokhi-Ardabili Effect of varying amounts of linseed oil or saturated fatty acids around insemination on reproductive and blood parameters of ewes Journal of Animal Science and Technology Linseed oil Saturated fatty acid (FA) Laparoscopic insemination Ewe Reproduction |
| title | Effect of varying amounts of linseed oil or saturated fatty acids around insemination on reproductive and blood parameters of ewes |
| title_full | Effect of varying amounts of linseed oil or saturated fatty acids around insemination on reproductive and blood parameters of ewes |
| title_fullStr | Effect of varying amounts of linseed oil or saturated fatty acids around insemination on reproductive and blood parameters of ewes |
| title_full_unstemmed | Effect of varying amounts of linseed oil or saturated fatty acids around insemination on reproductive and blood parameters of ewes |
| title_short | Effect of varying amounts of linseed oil or saturated fatty acids around insemination on reproductive and blood parameters of ewes |
| title_sort | effect of varying amounts of linseed oil or saturated fatty acids around insemination on reproductive and blood parameters of ewes |
| topic | Linseed oil Saturated fatty acid (FA) Laparoscopic insemination Ewe Reproduction |
| url | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e106 |
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