YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase
YgfY(SdhE/CptB) is highly conserved while has controversial functions in bacteria. It works as an antitoxin and composes a type IV toxin–antitoxin system with YgfX(CptA) typically in <i>Escherichia coli</i>, while functions as an flavinylation factor of succinate dehydrogenase and fumara...
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2316 |
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| author | Ming-Xing Zhang Kai-Li Zheng Ai-Guo Tang Xiao-Xia Hu Xin-Xin Guo Chao Wu Yuan-Yuan Cheng |
| author_facet | Ming-Xing Zhang Kai-Li Zheng Ai-Guo Tang Xiao-Xia Hu Xin-Xin Guo Chao Wu Yuan-Yuan Cheng |
| author_sort | Ming-Xing Zhang |
| collection | DOAJ |
| description | YgfY(SdhE/CptB) is highly conserved while has controversial functions in bacteria. It works as an antitoxin and composes a type IV toxin–antitoxin system with YgfX(CptA) typically in <i>Escherichia coli</i>, while functions as an flavinylation factor of succinate dehydrogenase and fumarate reductase typically in <i>Serratia</i> sp. In this study, we report the contribution of YgfY in <i>Shewanella oneidensis</i> MR-1 to tolerance of low temperature and nitrite. YgfY deficiency causes several growth defects of <i>S. oneidensis</i> MR-1 at low temperature, while YgfX do not cause a growth defect or morphological change of <i>S. oneidensis</i> MR1-1 and <i>E. coli</i>. YgfY do not interact with FtsZ and MreB nor with YgfX examined by bacterial two-hybrid assay. YgfY effect on growth under low temperature is not attributed to succinate dehydrogenase (SDH) because a mutant without SDH grows comparably with the wild-type strain in the presence of succinate. The <i>ygfY</i> mutant shows impaired tolerance to nitrite. Transcription of nitrite reductase and most ribosome proteins is significantly decreased in the <i>ygfY</i> mutant, which is consistent with the phenotypes detected above. Effects of YgfY on growth and nitrite tolerance are closely related to the RGXXE motif in YgfY. In summary, this study demonstrates pleiotropic impacts of YgfY in <i>S. oneidensis</i> MR-1, and sheds a light on the physiological versatility of YgfY in bacteria. |
| first_indexed | 2024-03-10T05:15:00Z |
| format | Article |
| id | doaj.art-abc70ead58b84d909a3d7599f8350147 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:15:00Z |
| publishDate | 2021-11-01 |
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| series | Microorganisms |
| spelling | doaj.art-abc70ead58b84d909a3d7599f83501472023-11-23T00:29:35ZengMDPI AGMicroorganisms2076-26072021-11-01911231610.3390/microorganisms9112316YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate DehydrogenaseMing-Xing Zhang0Kai-Li Zheng1Ai-Guo Tang2Xiao-Xia Hu3Xin-Xin Guo4Chao Wu5Yuan-Yuan Cheng6School of Life Sciences, Anhui University, Hefei 230602, ChinaSchool of Life Sciences, Anhui University, Hefei 230602, ChinaSchool of Life Sciences, Anhui University, Hefei 230602, ChinaSchool of Life Sciences, Anhui University, Hefei 230602, ChinaSchool of Life Sciences, Anhui University, Hefei 230602, ChinaSchool of Resources and Environment, Anhui University, Hefei 230602, ChinaSchool of Life Sciences, Anhui University, Hefei 230602, ChinaYgfY(SdhE/CptB) is highly conserved while has controversial functions in bacteria. It works as an antitoxin and composes a type IV toxin–antitoxin system with YgfX(CptA) typically in <i>Escherichia coli</i>, while functions as an flavinylation factor of succinate dehydrogenase and fumarate reductase typically in <i>Serratia</i> sp. In this study, we report the contribution of YgfY in <i>Shewanella oneidensis</i> MR-1 to tolerance of low temperature and nitrite. YgfY deficiency causes several growth defects of <i>S. oneidensis</i> MR-1 at low temperature, while YgfX do not cause a growth defect or morphological change of <i>S. oneidensis</i> MR1-1 and <i>E. coli</i>. YgfY do not interact with FtsZ and MreB nor with YgfX examined by bacterial two-hybrid assay. YgfY effect on growth under low temperature is not attributed to succinate dehydrogenase (SDH) because a mutant without SDH grows comparably with the wild-type strain in the presence of succinate. The <i>ygfY</i> mutant shows impaired tolerance to nitrite. Transcription of nitrite reductase and most ribosome proteins is significantly decreased in the <i>ygfY</i> mutant, which is consistent with the phenotypes detected above. Effects of YgfY on growth and nitrite tolerance are closely related to the RGXXE motif in YgfY. In summary, this study demonstrates pleiotropic impacts of YgfY in <i>S. oneidensis</i> MR-1, and sheds a light on the physiological versatility of YgfY in bacteria.https://www.mdpi.com/2076-2607/9/11/2316YgfY<i>Shewanella</i>toxin–antitoxinsuccinate dehydrogenaseflavinylation |
| spellingShingle | Ming-Xing Zhang Kai-Li Zheng Ai-Guo Tang Xiao-Xia Hu Xin-Xin Guo Chao Wu Yuan-Yuan Cheng YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase Microorganisms YgfY <i>Shewanella</i> toxin–antitoxin succinate dehydrogenase flavinylation |
| title | YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase |
| title_full | YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase |
| title_fullStr | YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase |
| title_full_unstemmed | YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase |
| title_short | YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase |
| title_sort | ygfy contributes to stress tolerance in i shewanella oneidensis i neither as an antitoxin nor as a flavinylation factor of succinate dehydrogenase |
| topic | YgfY <i>Shewanella</i> toxin–antitoxin succinate dehydrogenase flavinylation |
| url | https://www.mdpi.com/2076-2607/9/11/2316 |
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