In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes
Introduction: Diabetes mellitus (DM) has been recognized as the seventh leading cause of global mortality; however, researchers seek alternative means to manage the menace. The current study sought to investigate antioxidant potentials, α-amylase, and α-glucosidase inhibitory activities of ethanolic...
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| Format: | Article |
| Language: | English |
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Shahrekord University of Medical Sciences
2021-10-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | http://herbmedpharmacol.com/PDF/jhp-10-408.pdf |
| _version_ | 1819106163111755776 |
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| author | Oluwaseun Ruth Olasehinde Olakunle Bamikole Afolabi Benjamin Olusola Omiyale Oyindamola Adeniyi Olaoye |
| author_facet | Oluwaseun Ruth Olasehinde Olakunle Bamikole Afolabi Benjamin Olusola Omiyale Oyindamola Adeniyi Olaoye |
| author_sort | Oluwaseun Ruth Olasehinde |
| collection | DOAJ |
| description | Introduction: Diabetes mellitus (DM) has been recognized as the seventh leading cause of global mortality; however, researchers seek alternative means to manage the menace. The current study sought to investigate antioxidant potentials, α-amylase, and α-glucosidase inhibitory activities of ethanolic extract of Moringa oleifera flower in vitro. Methods: Antioxidant properties of the extract were appraised by assessing its inhibition against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH•), and hydrogen peroxide (H2O2) free radicals, as well as ferric reducing antioxidant power (FRAP), the antidiabetic activity was evaluated by α-amylase and α-glucosidase inhibition.Results: In this study, ethanolic extract of M. oleifera flower demonstrated a significant (P < 0.05) inhibition against DPPH free radical (43.57–83.56%) in a concentration-dependent manner, while FRAP (101.76 ± 1.63 mg/100 g), OH• scavenging ability (71.62 ± 0.95 mg/100 g), and H2O2 free radical scavenging capacity (15.33 ± 1.20 mg/100 g) were also observed. In the same manner, ethanolic extract of M. oleifera flower revealed a significant (P < 0.05) inhibition against α-amylase (IC50= 37.63 mg/mL) and α-glucosidase activities (IC50= 38.30 mg/mL) in the presence of their respective substrates in a concentration-dependent manner in comparison with acarbose. Conclusion: Ethanoic extract of M. oleifera flower could be useful as an alternative phytotherapy in the management of DM, having shown a strong antioxidative capacity and substantial inhibition against the activities of key enzymes involved in carbohydrate hydrolysis in vitro. |
| first_indexed | 2024-12-22T02:33:46Z |
| format | Article |
| id | doaj.art-abc8418a1b424a38a32dd7e967670474 |
| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-12-22T02:33:46Z |
| publishDate | 2021-10-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-abc8418a1b424a38a32dd7e9676704742022-12-21T18:41:49ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042021-10-0110440841410.34172/jhp.2021.48jhp-37923In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetesOluwaseun Ruth Olasehinde0Olakunle Bamikole Afolabi1Benjamin Olusola Omiyale2Oyindamola Adeniyi Olaoye3College of Science, Department of Chemical Sciences, Biochemistry Programme, Afe Babalola University, P.M.B 5454 Ado-Ekiti, Ekiti State, NigeriaCollege of Science, Department of Chemical Sciences, Biochemistry Programme, Afe Babalola University, P.M.B 5454 Ado-Ekiti, Ekiti State, NigeriaCollege of Science, Department of Chemical Sciences, Biochemistry Programme, Afe Babalola University, P.M.B 5454 Ado-Ekiti, Ekiti State, NigeriaCollege of Science, Department of Chemical Sciences, Biochemistry Programme, Afe Babalola University, P.M.B 5454 Ado-Ekiti, Ekiti State, NigeriaIntroduction: Diabetes mellitus (DM) has been recognized as the seventh leading cause of global mortality; however, researchers seek alternative means to manage the menace. The current study sought to investigate antioxidant potentials, α-amylase, and α-glucosidase inhibitory activities of ethanolic extract of Moringa oleifera flower in vitro. Methods: Antioxidant properties of the extract were appraised by assessing its inhibition against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH•), and hydrogen peroxide (H2O2) free radicals, as well as ferric reducing antioxidant power (FRAP), the antidiabetic activity was evaluated by α-amylase and α-glucosidase inhibition.Results: In this study, ethanolic extract of M. oleifera flower demonstrated a significant (P < 0.05) inhibition against DPPH free radical (43.57–83.56%) in a concentration-dependent manner, while FRAP (101.76 ± 1.63 mg/100 g), OH• scavenging ability (71.62 ± 0.95 mg/100 g), and H2O2 free radical scavenging capacity (15.33 ± 1.20 mg/100 g) were also observed. In the same manner, ethanolic extract of M. oleifera flower revealed a significant (P < 0.05) inhibition against α-amylase (IC50= 37.63 mg/mL) and α-glucosidase activities (IC50= 38.30 mg/mL) in the presence of their respective substrates in a concentration-dependent manner in comparison with acarbose. Conclusion: Ethanoic extract of M. oleifera flower could be useful as an alternative phytotherapy in the management of DM, having shown a strong antioxidative capacity and substantial inhibition against the activities of key enzymes involved in carbohydrate hydrolysis in vitro.http://herbmedpharmacol.com/PDF/jhp-10-408.pdfmoringa oleiferaantioxidant activityα-amylaseα-glucosidasetype ii diabetes |
| spellingShingle | Oluwaseun Ruth Olasehinde Olakunle Bamikole Afolabi Benjamin Olusola Omiyale Oyindamola Adeniyi Olaoye In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes Journal of HerbMed Pharmacology moringa oleifera antioxidant activity α-amylase α-glucosidase type ii diabetes |
| title | In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes |
| title_full | In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes |
| title_fullStr | In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes |
| title_full_unstemmed | In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes |
| title_short | In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes |
| title_sort | in vitro inhibitory potentials of ethanolic extract of moringa oleifera flower against enzymes activities linked to diabetes |
| topic | moringa oleifera antioxidant activity α-amylase α-glucosidase type ii diabetes |
| url | http://herbmedpharmacol.com/PDF/jhp-10-408.pdf |
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