Chemical modification of b-lactoglobulin by quinones
The avarone/avarol quinone/hydroquinone couple, as well as their derivatives show considerable antitumor activity. In this work, covalent modifications of b-lactoglobulin, isolated from cow milk, by avarone, its model compound 2-tert-butyl-1,4-benzoquinone, and several of their alkylthio derivatives...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2003-05-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.shd.org.yu/HtDocs/SHD/Vol68/No4-5/V68-No4_5-01.pdf |
| _version_ | 1817994289331830784 |
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| author | DUSAN SLADIC NENAD MILOSAVIC NATASA BOZIC TATJANA BOZIC ZORAN VUJCIC IRENA NOVAKOVIC |
| author_facet | DUSAN SLADIC NENAD MILOSAVIC NATASA BOZIC TATJANA BOZIC ZORAN VUJCIC IRENA NOVAKOVIC |
| author_sort | DUSAN SLADIC |
| collection | DOAJ |
| description | The avarone/avarol quinone/hydroquinone couple, as well as their derivatives show considerable antitumor activity. In this work, covalent modifications of b-lactoglobulin, isolated from cow milk, by avarone, its model compound 2-tert-butyl-1,4-benzoquinone, and several of their alkylthio derivatives were studied. The techniques applied for assaying the modifications were: UV/VIS spectrophotometry, SDS PAGE and isoelectrofocusing. The results of the SDS PAGE suggest that polymerisation of the protein occurs. The shift of the pI of the protein upon modification toward lower values indicates that lysine amino groups are the principal site of the reaction of b-lactoglobulin with the quinones. |
| first_indexed | 2024-04-14T01:50:58Z |
| format | Article |
| id | doaj.art-abc85607fefe441a875f28e05010f267 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 |
| language | English |
| last_indexed | 2024-04-14T01:50:58Z |
| publishDate | 2003-05-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-abc85607fefe441a875f28e05010f2672022-12-22T02:19:22ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51392003-05-01684-5243248Chemical modification of b-lactoglobulin by quinonesDUSAN SLADICNENAD MILOSAVICNATASA BOZICTATJANA BOZICZORAN VUJCICIRENA NOVAKOVICThe avarone/avarol quinone/hydroquinone couple, as well as their derivatives show considerable antitumor activity. In this work, covalent modifications of b-lactoglobulin, isolated from cow milk, by avarone, its model compound 2-tert-butyl-1,4-benzoquinone, and several of their alkylthio derivatives were studied. The techniques applied for assaying the modifications were: UV/VIS spectrophotometry, SDS PAGE and isoelectrofocusing. The results of the SDS PAGE suggest that polymerisation of the protein occurs. The shift of the pI of the protein upon modification toward lower values indicates that lysine amino groups are the principal site of the reaction of b-lactoglobulin with the quinones.http://www.shd.org.yu/HtDocs/SHD/Vol68/No4-5/V68-No4_5-01.pdfavaronequinoneb-lactoglobulincovalent modification |
| spellingShingle | DUSAN SLADIC NENAD MILOSAVIC NATASA BOZIC TATJANA BOZIC ZORAN VUJCIC IRENA NOVAKOVIC Chemical modification of b-lactoglobulin by quinones Journal of the Serbian Chemical Society avarone quinone b-lactoglobulin covalent modification |
| title | Chemical modification of b-lactoglobulin by quinones |
| title_full | Chemical modification of b-lactoglobulin by quinones |
| title_fullStr | Chemical modification of b-lactoglobulin by quinones |
| title_full_unstemmed | Chemical modification of b-lactoglobulin by quinones |
| title_short | Chemical modification of b-lactoglobulin by quinones |
| title_sort | chemical modification of b lactoglobulin by quinones |
| topic | avarone quinone b-lactoglobulin covalent modification |
| url | http://www.shd.org.yu/HtDocs/SHD/Vol68/No4-5/V68-No4_5-01.pdf |
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