Evaluation of Neck Lymph Node Metastasis on Contrast-Enhanced Ultrasound: An Animal Study
Objectives To assess the performance of contrast-enhanced ultrasound (CEUS) intended to differentiate hyperplastic from malignant neck lymph nodes in an animal model. Methods Twenty-four New Zealand rabbits were randomly divided into two groups: neck lymph node metastasis group (12 rabbits) and reac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Otorhinolaryngology-Head and Neck Surgery
2017-03-01
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| Series: | Clinical and Experimental Otorhinolaryngology |
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| Online Access: | http://www.e-ceo.org/upload/pdf/ceo-2015-01284.pdf |
| _version_ | 1817997507365437440 |
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| author | Zheng Cui Yi Gao Weihua Wang Zhenghua Zhu Yu Zhang Zhaoxin Ma |
| author_facet | Zheng Cui Yi Gao Weihua Wang Zhenghua Zhu Yu Zhang Zhaoxin Ma |
| author_sort | Zheng Cui |
| collection | DOAJ |
| description | Objectives To assess the performance of contrast-enhanced ultrasound (CEUS) intended to differentiate hyperplastic from malignant neck lymph nodes in an animal model. Methods Twenty-four New Zealand rabbits were randomly divided into two groups: neck lymph node metastasis group (12 rabbits) and reactive hyperplastic lymph node group (12 rabbits). Tongue VX2 carcinoma with cervical lymph node metastasis was induced in 12 rabbits by injecting VX2 carcinoma suspension into the left tongue submucosa. Hyperplastic neck lymph nodes were induced by injecting egg yolk in the submandibular region of the rabbits in hyperplastic group. CEUS were performed in both groups before and after intravenous administration of SonoVue. The site, number, echogenicity, longitudinal and transverse nodal dimensions, patterns of enhancement of the neck lymph nodes were observed and recorded. Results In both groups only one lymph node was found in the left (tumor) side of the neck. CEUS found 12 of 12 metastatic lymph nodes in metastasis group, and diagnosed 11 of 12 lymph nodes as metastatic. Histopathologic analysis revealed metastatic lesions in all 12 rabbits, each with one lymph node, and all 12 lymph nodes in hyperplastic group is inflammation lymph nodes. All 12 cases in the hyperplastic group showed centripetal homogeneous enhancement while in the metastasis group one case showed centripetal homogeneous enhancement, three cases showed centrifugal heterogeneous enhancement, and eight cases showed diffused heterogeneous enhancement. Only one lymph node was dissected on the left side of the neck in each rabbit in both groups. Conclusion CEUS can play a role in discriminating metastatic from hyperplastic lymph nodes in head and neck carcinoma. |
| first_indexed | 2024-04-14T02:39:15Z |
| format | Article |
| id | doaj.art-abc87dcc630241019f67e1316b6e18fe |
| institution | Directory Open Access Journal |
| issn | 1976-8710 2005-0720 |
| language | English |
| last_indexed | 2024-04-14T02:39:15Z |
| publishDate | 2017-03-01 |
| publisher | Korean Society of Otorhinolaryngology-Head and Neck Surgery |
| record_format | Article |
| series | Clinical and Experimental Otorhinolaryngology |
| spelling | doaj.art-abc87dcc630241019f67e1316b6e18fe2022-12-22T02:17:13ZengKorean Society of Otorhinolaryngology-Head and Neck SurgeryClinical and Experimental Otorhinolaryngology1976-87102005-07202017-03-0110110911410.21053/ceo.2015.01284488Evaluation of Neck Lymph Node Metastasis on Contrast-Enhanced Ultrasound: An Animal StudyZheng Cui0Yi Gao1Weihua Wang2Zhenghua Zhu3Yu Zhang4Zhaoxin Ma5 Department of Ultrasound Diagnosis, East Hospital, Tongji University School of Medicine, Shanghai, China Department of Otorhinolaryngology-Head and Neck Surgery, East Hospital, Tongji University School of Medicine, Shanghai, China Department of Otorhinolaryngology-Head and Neck Surgery, East Hospital, Tongji University School of Medicine, Shanghai, China Department of Otorhinolaryngology-Head and Neck Surgery, East Hospital, Tongji University School of Medicine, Shanghai, China Department of Otorhinolaryngology-Head and Neck Surgery, East Hospital, Tongji University School of Medicine, Shanghai, China Department of Otorhinolaryngology-Head and Neck Surgery, East Hospital, Tongji University School of Medicine, Shanghai, ChinaObjectives To assess the performance of contrast-enhanced ultrasound (CEUS) intended to differentiate hyperplastic from malignant neck lymph nodes in an animal model. Methods Twenty-four New Zealand rabbits were randomly divided into two groups: neck lymph node metastasis group (12 rabbits) and reactive hyperplastic lymph node group (12 rabbits). Tongue VX2 carcinoma with cervical lymph node metastasis was induced in 12 rabbits by injecting VX2 carcinoma suspension into the left tongue submucosa. Hyperplastic neck lymph nodes were induced by injecting egg yolk in the submandibular region of the rabbits in hyperplastic group. CEUS were performed in both groups before and after intravenous administration of SonoVue. The site, number, echogenicity, longitudinal and transverse nodal dimensions, patterns of enhancement of the neck lymph nodes were observed and recorded. Results In both groups only one lymph node was found in the left (tumor) side of the neck. CEUS found 12 of 12 metastatic lymph nodes in metastasis group, and diagnosed 11 of 12 lymph nodes as metastatic. Histopathologic analysis revealed metastatic lesions in all 12 rabbits, each with one lymph node, and all 12 lymph nodes in hyperplastic group is inflammation lymph nodes. All 12 cases in the hyperplastic group showed centripetal homogeneous enhancement while in the metastasis group one case showed centripetal homogeneous enhancement, three cases showed centrifugal heterogeneous enhancement, and eight cases showed diffused heterogeneous enhancement. Only one lymph node was dissected on the left side of the neck in each rabbit in both groups. Conclusion CEUS can play a role in discriminating metastatic from hyperplastic lymph nodes in head and neck carcinoma.http://www.e-ceo.org/upload/pdf/ceo-2015-01284.pdfUltrasonographyHead and Neck NeoplasmsLymph Node Metastasis |
| spellingShingle | Zheng Cui Yi Gao Weihua Wang Zhenghua Zhu Yu Zhang Zhaoxin Ma Evaluation of Neck Lymph Node Metastasis on Contrast-Enhanced Ultrasound: An Animal Study Clinical and Experimental Otorhinolaryngology Ultrasonography Head and Neck Neoplasms Lymph Node Metastasis |
| title | Evaluation of Neck Lymph Node Metastasis on Contrast-Enhanced Ultrasound: An Animal Study |
| title_full | Evaluation of Neck Lymph Node Metastasis on Contrast-Enhanced Ultrasound: An Animal Study |
| title_fullStr | Evaluation of Neck Lymph Node Metastasis on Contrast-Enhanced Ultrasound: An Animal Study |
| title_full_unstemmed | Evaluation of Neck Lymph Node Metastasis on Contrast-Enhanced Ultrasound: An Animal Study |
| title_short | Evaluation of Neck Lymph Node Metastasis on Contrast-Enhanced Ultrasound: An Animal Study |
| title_sort | evaluation of neck lymph node metastasis on contrast enhanced ultrasound an animal study |
| topic | Ultrasonography Head and Neck Neoplasms Lymph Node Metastasis |
| url | http://www.e-ceo.org/upload/pdf/ceo-2015-01284.pdf |
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