CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions
The CRISPR genome editing technology is a crucial tool for enabling revolutionary advancements in plant genetic improvement. This review shows the latest developments in CRISPR/Cas9 genome editing system variants, discussing their benefits and limitations for plant improvement. While this technology...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/22/16241 |
| _version_ | 1797459073521680384 |
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| author | Ali Movahedi Soheila Aghaei-Dargiri Hongyan Li Qiang Zhuge Weibo Sun |
| author_facet | Ali Movahedi Soheila Aghaei-Dargiri Hongyan Li Qiang Zhuge Weibo Sun |
| author_sort | Ali Movahedi |
| collection | DOAJ |
| description | The CRISPR genome editing technology is a crucial tool for enabling revolutionary advancements in plant genetic improvement. This review shows the latest developments in CRISPR/Cas9 genome editing system variants, discussing their benefits and limitations for plant improvement. While this technology presents immense opportunities for plant breeding, it also raises serious biosafety concerns that require careful consideration, including potential off-target effects and the unintended transfer of modified genes to other organisms. This paper highlights strategies to mitigate biosafety risks and explores innovative plant gene editing detection methods. Our review investigates the international biosafety guidelines for gene-edited crops, analyzing their broad implications for agricultural and biotechnology research and advancement. We hope to provide illuminating and refined perspectives for industry practitioners and policymakers by evaluating CRISPR genome enhancement in plants. |
| first_indexed | 2024-03-09T16:46:11Z |
| format | Article |
| id | doaj.art-abc98cf317a44291903cb7ef3b843a5f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:46:11Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-abc98cf317a44291903cb7ef3b843a5f2023-11-24T14:46:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221624110.3390/ijms242216241CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future DirectionsAli Movahedi0Soheila Aghaei-Dargiri1Hongyan Li2Qiang Zhuge3Weibo Sun4Department of Biology and the Environment, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Biological Control Research, Iranian Research Institute of Plant Protection, Agricultural Research Education and Extension Organization (AREEO), Tehran 19858-13111, IranDepartment of Biology and the Environment, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Biology and the Environment, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Biology and the Environment, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, ChinaThe CRISPR genome editing technology is a crucial tool for enabling revolutionary advancements in plant genetic improvement. This review shows the latest developments in CRISPR/Cas9 genome editing system variants, discussing their benefits and limitations for plant improvement. While this technology presents immense opportunities for plant breeding, it also raises serious biosafety concerns that require careful consideration, including potential off-target effects and the unintended transfer of modified genes to other organisms. This paper highlights strategies to mitigate biosafety risks and explores innovative plant gene editing detection methods. Our review investigates the international biosafety guidelines for gene-edited crops, analyzing their broad implications for agricultural and biotechnology research and advancement. We hope to provide illuminating and refined perspectives for industry practitioners and policymakers by evaluating CRISPR genome enhancement in plants.https://www.mdpi.com/1422-0067/24/22/16241CRISPR variantsgene editingplant genetic improvementbiosafetyoff-target effects |
| spellingShingle | Ali Movahedi Soheila Aghaei-Dargiri Hongyan Li Qiang Zhuge Weibo Sun CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions International Journal of Molecular Sciences CRISPR variants gene editing plant genetic improvement biosafety off-target effects |
| title | CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions |
| title_full | CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions |
| title_fullStr | CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions |
| title_full_unstemmed | CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions |
| title_short | CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions |
| title_sort | crispr variants for gene editing in plants biosafety risks and future directions |
| topic | CRISPR variants gene editing plant genetic improvement biosafety off-target effects |
| url | https://www.mdpi.com/1422-0067/24/22/16241 |
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