Synthesis of Silver Nanoparticles by Leaf Extract of <i>Cucumis melo</i> L. and Their In Vitro Antidiabetic and Anticoccidial Activities
In this study, silver nanoparticles were synthesized using <i>Cucumis melo</i> L. leaf extract via a green synthesis approach and their potential against diabetes and coccidiosis was tested under in vitro conditions. The phytochemical components in the leaf extract reacted with silver ni...
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MDPI AG
2023-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/13/4995 |
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| author | Pushpa Rani Naveen Kumar Kantharaj Perinmbam Sandhanasamy Devanesan Mohamad S. AlSalhi Nassar Asemi Marcello Nicoletti |
| author_facet | Pushpa Rani Naveen Kumar Kantharaj Perinmbam Sandhanasamy Devanesan Mohamad S. AlSalhi Nassar Asemi Marcello Nicoletti |
| author_sort | Pushpa Rani |
| collection | DOAJ |
| description | In this study, silver nanoparticles were synthesized using <i>Cucumis melo</i> L. leaf extract via a green synthesis approach and their potential against diabetes and coccidiosis was tested under in vitro conditions. The phytochemical components in the leaf extract reacted with silver nitrate in solution and yielded <i>C. melo</i>-silver nanoparticles (<i>Cm</i>-AgNPs). The synthesis of AgNPs was confirmed via UV–visible spectroscopy by obtaining a peak at 440 nm. The nanoparticles were characterized by their morphology, crystallinity, and the presence of functional groups. In vitro α-amylase and α-glucosidase inhibition assays were carried out at different concentrations in the range of 20 to 100 μg/mL of <i>Cm</i>-AgNPs. The <i>Cm</i>-AgNPs exhibited enzyme inhibitory activity in a concentration-dependent manner. As the concentration of <i>Cm</i>-AgNPs increased the inhibitory activities were also increased linearly and the highest inhibition was observed at 100 μg/mL. The effectiveness of <i>Cm</i>-AgNPs against <i>Eimeria tenalla</i> was assessed by an in vitro 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay using Madin–Darby bovine kidney (MDBK) cell lines. The results revealed that the viability of the oocysts and further sporulation were decreased with the increased concentration of <i>Cm</i>-AgNPs. The AgNPs synthesized from the <i>C. melo</i> leaf extract have shown promising potential against diabetes and coccidiosis, and they could be used in biomedical applications. |
| first_indexed | 2024-03-11T01:34:26Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T01:34:26Z |
| publishDate | 2023-06-01 |
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| series | Molecules |
| spelling | doaj.art-abccd326298b4c83a6bf9e1560c2801b2023-11-18T17:06:38ZengMDPI AGMolecules1420-30492023-06-012813499510.3390/molecules28134995Synthesis of Silver Nanoparticles by Leaf Extract of <i>Cucumis melo</i> L. and Their In Vitro Antidiabetic and Anticoccidial ActivitiesPushpa Rani0Naveen Kumar1Kantharaj Perinmbam2Sandhanasamy Devanesan3Mohamad S. AlSalhi4Nassar Asemi5Marcello Nicoletti6Department of Advanced Zoology and Biotechnology, Loyola College, Chennai 600034, Tamil Nadu, IndiaDepartment of Advanced Zoology and Biotechnology, Loyola College, Chennai 600034, Tamil Nadu, IndiaPG and Research Department of Botany, Government Arts College for Men (Autonomous), Affiliated to University of Madras, Nandanam, Chennai 600035, Tamil Nadu, IndiaDepartment of Physics and Astronomy, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Physics and Astronomy, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Physics and Astronomy, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalyIn this study, silver nanoparticles were synthesized using <i>Cucumis melo</i> L. leaf extract via a green synthesis approach and their potential against diabetes and coccidiosis was tested under in vitro conditions. The phytochemical components in the leaf extract reacted with silver nitrate in solution and yielded <i>C. melo</i>-silver nanoparticles (<i>Cm</i>-AgNPs). The synthesis of AgNPs was confirmed via UV–visible spectroscopy by obtaining a peak at 440 nm. The nanoparticles were characterized by their morphology, crystallinity, and the presence of functional groups. In vitro α-amylase and α-glucosidase inhibition assays were carried out at different concentrations in the range of 20 to 100 μg/mL of <i>Cm</i>-AgNPs. The <i>Cm</i>-AgNPs exhibited enzyme inhibitory activity in a concentration-dependent manner. As the concentration of <i>Cm</i>-AgNPs increased the inhibitory activities were also increased linearly and the highest inhibition was observed at 100 μg/mL. The effectiveness of <i>Cm</i>-AgNPs against <i>Eimeria tenalla</i> was assessed by an in vitro 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay using Madin–Darby bovine kidney (MDBK) cell lines. The results revealed that the viability of the oocysts and further sporulation were decreased with the increased concentration of <i>Cm</i>-AgNPs. The AgNPs synthesized from the <i>C. melo</i> leaf extract have shown promising potential against diabetes and coccidiosis, and they could be used in biomedical applications.https://www.mdpi.com/1420-3049/28/13/4995silver nanoparticles<i>Cucumis melo</i>diabetescoccidiosisα-amylaseα-glucosidase |
| spellingShingle | Pushpa Rani Naveen Kumar Kantharaj Perinmbam Sandhanasamy Devanesan Mohamad S. AlSalhi Nassar Asemi Marcello Nicoletti Synthesis of Silver Nanoparticles by Leaf Extract of <i>Cucumis melo</i> L. and Their In Vitro Antidiabetic and Anticoccidial Activities Molecules silver nanoparticles <i>Cucumis melo</i> diabetes coccidiosis α-amylase α-glucosidase |
| title | Synthesis of Silver Nanoparticles by Leaf Extract of <i>Cucumis melo</i> L. and Their In Vitro Antidiabetic and Anticoccidial Activities |
| title_full | Synthesis of Silver Nanoparticles by Leaf Extract of <i>Cucumis melo</i> L. and Their In Vitro Antidiabetic and Anticoccidial Activities |
| title_fullStr | Synthesis of Silver Nanoparticles by Leaf Extract of <i>Cucumis melo</i> L. and Their In Vitro Antidiabetic and Anticoccidial Activities |
| title_full_unstemmed | Synthesis of Silver Nanoparticles by Leaf Extract of <i>Cucumis melo</i> L. and Their In Vitro Antidiabetic and Anticoccidial Activities |
| title_short | Synthesis of Silver Nanoparticles by Leaf Extract of <i>Cucumis melo</i> L. and Their In Vitro Antidiabetic and Anticoccidial Activities |
| title_sort | synthesis of silver nanoparticles by leaf extract of i cucumis melo i l and their in vitro antidiabetic and anticoccidial activities |
| topic | silver nanoparticles <i>Cucumis melo</i> diabetes coccidiosis α-amylase α-glucosidase |
| url | https://www.mdpi.com/1420-3049/28/13/4995 |
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