Promoting Heme and Phycocyanin Biosynthesis in <i>Synechocystis</i> sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering
Due to their unique biochemical and spectroscopic properties, both heme and phycocyanobilin are widely applied in the medical and food industries. <i>Synechocystis</i> sp. PCC 6803 contains both heme and phycocyanin, and is capable of synthesizing phycocyanin using heme as a precursor. T...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/21/7/403 |
| _version_ | 1797588510466637824 |
|---|---|
| author | Kai Cao Xiaodong Wang Fengjie Sun Hao Zhang Yulin Cui Yujiao Cao Qingshou Yao Xiangyu Zhu Ting Yao Meng Wang Chunxiao Meng Zhengquan Gao |
| author_facet | Kai Cao Xiaodong Wang Fengjie Sun Hao Zhang Yulin Cui Yujiao Cao Qingshou Yao Xiangyu Zhu Ting Yao Meng Wang Chunxiao Meng Zhengquan Gao |
| author_sort | Kai Cao |
| collection | DOAJ |
| description | Due to their unique biochemical and spectroscopic properties, both heme and phycocyanobilin are widely applied in the medical and food industries. <i>Synechocystis</i> sp. PCC 6803 contains both heme and phycocyanin, and is capable of synthesizing phycocyanin using heme as a precursor. The aim of this study was to uncover viable metabolic targets in the porphyrin pathway from <i>Synechocystis</i> sp. PCC 6803 to promote the accumulation of heme and phycocyanin in the recombinant strains of microalgae. A total of 10 genes related to heme synthesis pathway derived from <i>Synechococcus elongatus</i> PCC 7942 and 12 genes related to endogenous heme synthesis were individually overexpressed in strain PCC 6803. The growth rate and pigment content (heme, phycocyanin, chlorophyll a and carotenoids) of 22 recombinant algal strains were characterized. Quantitative real-time PCR technology was used to investigate the molecular mechanisms underlying the changes in physiological indicators in the recombinant algal strains. Among the 22 mutant strains, the mutant overexpressing the haemoglobin gene (<i>glbN</i>) of strain PCC 6803 had the highest heme content, which was 2.5 times higher than the wild type; the mutant overexpressing the gene of strain PCC 7942 (<i>hemF</i>) had the highest phycocyanin content, which was 4.57 times higher than the wild type. Overall, the results suggest that genes in the porphyrin pathway could significantly affect the heme and phycocyanin content in strain PCC 6803. Our study provides novel crucial targets for promoting the accumulation of heme and phycocyanin in cyanobacteria. |
| first_indexed | 2024-03-11T00:53:05Z |
| format | Article |
| id | doaj.art-abd017f208eb4482b4f190b3c13e76a7 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T00:53:05Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-abd017f208eb4482b4f190b3c13e76a72023-11-18T20:14:19ZengMDPI AGMarine Drugs1660-33972023-07-0121740310.3390/md21070403Promoting Heme and Phycocyanin Biosynthesis in <i>Synechocystis</i> sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic EngineeringKai Cao0Xiaodong Wang1Fengjie Sun2Hao Zhang3Yulin Cui4Yujiao Cao5Qingshou Yao6Xiangyu Zhu7Ting Yao8Meng Wang9Chunxiao Meng10Zhengquan Gao11School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255049, ChinaSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo 255049, ChinaDepartment of Biological Sciences, School of Science and Technology, Georgia Gwinnett College, Lawrenceville, GA 30043, USASchool of Pharmacy, Binzhou Medical University, Yantai 264003, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai 264003, ChinaSchool of Foreign Languages, Shandong University of Technology, Zibo 255090, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai 264003, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai 264003, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai 264003, ChinaYantai Hongyuan Bio-Fertilizer Co., Ltd., Yantai 264000, ChinaSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo 255049, ChinaSchool of Life Sciences and Medicine, Shandong University of Technology, Zibo 255049, ChinaDue to their unique biochemical and spectroscopic properties, both heme and phycocyanobilin are widely applied in the medical and food industries. <i>Synechocystis</i> sp. PCC 6803 contains both heme and phycocyanin, and is capable of synthesizing phycocyanin using heme as a precursor. The aim of this study was to uncover viable metabolic targets in the porphyrin pathway from <i>Synechocystis</i> sp. PCC 6803 to promote the accumulation of heme and phycocyanin in the recombinant strains of microalgae. A total of 10 genes related to heme synthesis pathway derived from <i>Synechococcus elongatus</i> PCC 7942 and 12 genes related to endogenous heme synthesis were individually overexpressed in strain PCC 6803. The growth rate and pigment content (heme, phycocyanin, chlorophyll a and carotenoids) of 22 recombinant algal strains were characterized. Quantitative real-time PCR technology was used to investigate the molecular mechanisms underlying the changes in physiological indicators in the recombinant algal strains. Among the 22 mutant strains, the mutant overexpressing the haemoglobin gene (<i>glbN</i>) of strain PCC 6803 had the highest heme content, which was 2.5 times higher than the wild type; the mutant overexpressing the gene of strain PCC 7942 (<i>hemF</i>) had the highest phycocyanin content, which was 4.57 times higher than the wild type. Overall, the results suggest that genes in the porphyrin pathway could significantly affect the heme and phycocyanin content in strain PCC 6803. Our study provides novel crucial targets for promoting the accumulation of heme and phycocyanin in cyanobacteria.https://www.mdpi.com/1660-3397/21/7/403hemephycocyaningene expressionsynthetic mechanism<i>Synechocystis</i> sp. PCC 6803<i>Synechococcus</i> elongatus PCC 7942 |
| spellingShingle | Kai Cao Xiaodong Wang Fengjie Sun Hao Zhang Yulin Cui Yujiao Cao Qingshou Yao Xiangyu Zhu Ting Yao Meng Wang Chunxiao Meng Zhengquan Gao Promoting Heme and Phycocyanin Biosynthesis in <i>Synechocystis</i> sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering Marine Drugs heme phycocyanin gene expression synthetic mechanism <i>Synechocystis</i> sp. PCC 6803 <i>Synechococcus</i> elongatus PCC 7942 |
| title | Promoting Heme and Phycocyanin Biosynthesis in <i>Synechocystis</i> sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering |
| title_full | Promoting Heme and Phycocyanin Biosynthesis in <i>Synechocystis</i> sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering |
| title_fullStr | Promoting Heme and Phycocyanin Biosynthesis in <i>Synechocystis</i> sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering |
| title_full_unstemmed | Promoting Heme and Phycocyanin Biosynthesis in <i>Synechocystis</i> sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering |
| title_short | Promoting Heme and Phycocyanin Biosynthesis in <i>Synechocystis</i> sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering |
| title_sort | promoting heme and phycocyanin biosynthesis in i synechocystis i sp pcc 6803 by overexpression of porphyrin pathway genes with genetic engineering |
| topic | heme phycocyanin gene expression synthetic mechanism <i>Synechocystis</i> sp. PCC 6803 <i>Synechococcus</i> elongatus PCC 7942 |
| url | https://www.mdpi.com/1660-3397/21/7/403 |
| work_keys_str_mv | AT kaicao promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT xiaodongwang promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT fengjiesun promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT haozhang promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT yulincui promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT yujiaocao promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT qingshouyao promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT xiangyuzhu promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT tingyao promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT mengwang promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT chunxiaomeng promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering AT zhengquangao promotinghemeandphycocyaninbiosynthesisinisynechocystisisppcc6803byoverexpressionofporphyrinpathwaygeneswithgeneticengineering |