MicroRNA Expression Profiles Related to Early Stage Murine Concanavalin A-Induced Hepatitis
Background: Fulminant hepatitis is a severe liver disease characterized by massive hepatocyte necrosis and clinical signs of liver failure. This study explores the expression profile of microRNAs, which are regulators of a number of pathophysiological processes, during the early stage of concanavali...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2014-06-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/362970 |
| _version_ | 1818993141970108416 |
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| author | Hong-Yu Jia Feng Chen Jian-Zhong Chen Shan-Shan Wu Jing Wang Qing-Yi Cao Zhi Chen Hai-Hong Zhu |
| author_facet | Hong-Yu Jia Feng Chen Jian-Zhong Chen Shan-Shan Wu Jing Wang Qing-Yi Cao Zhi Chen Hai-Hong Zhu |
| author_sort | Hong-Yu Jia |
| collection | DOAJ |
| description | Background: Fulminant hepatitis is a severe liver disease characterized by massive hepatocyte necrosis and clinical signs of liver failure. This study explores the expression profile of microRNAs, which are regulators of a number of pathophysiological processes, during the early stage of concanavalin A (Con A)-induced hepatitis. Methods: Balb/c mice were given ConA injections to induce fulminant hepatitis. miRNA expression profiling in liver tissues was carried out by microarray analysis. The differentially expressed miRNAs were subjected to time sequence profile analysis, gene-miRNA regulatory network analysis, and gene ontology-miRNA regulatory network analysis. Results: Eleven miRNAs among multiClass were found to be significantly differentially expressed between liver tissue in early stage fulminant hepatitis and normal control liver tissue. Mmu-miR-133a was the most differentially expressed with the strongest regulatory ability, regulating 47 mRNAs. Mmu-miR-10a was the most highly expressed in the microRNA-GO-Network and also exerted a strong regulatory ability. The expression profiles of miR-133a and miR-10a were verified by RT-PCR. Conclusions: These results show that, in the early stage, ConA-induced fulminant hepatitis induces a distinct miRNA expression profile. This differential miRNA expression profile may provide pathogenic clues and potential diagnostic and prognostic markers in acute and severe liver disease. |
| first_indexed | 2024-12-20T20:37:21Z |
| format | Article |
| id | doaj.art-abd15d4e8523445585d7c1662f7ce7b3 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-20T20:37:21Z |
| publishDate | 2014-06-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-abd15d4e8523445585d7c1662f7ce7b32022-12-21T19:27:11ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-06-013361933194410.1159/000362970362970MicroRNA Expression Profiles Related to Early Stage Murine Concanavalin A-Induced HepatitisHong-Yu JiaFeng ChenJian-Zhong ChenShan-Shan WuJing WangQing-Yi CaoZhi ChenHai-Hong ZhuBackground: Fulminant hepatitis is a severe liver disease characterized by massive hepatocyte necrosis and clinical signs of liver failure. This study explores the expression profile of microRNAs, which are regulators of a number of pathophysiological processes, during the early stage of concanavalin A (Con A)-induced hepatitis. Methods: Balb/c mice were given ConA injections to induce fulminant hepatitis. miRNA expression profiling in liver tissues was carried out by microarray analysis. The differentially expressed miRNAs were subjected to time sequence profile analysis, gene-miRNA regulatory network analysis, and gene ontology-miRNA regulatory network analysis. Results: Eleven miRNAs among multiClass were found to be significantly differentially expressed between liver tissue in early stage fulminant hepatitis and normal control liver tissue. Mmu-miR-133a was the most differentially expressed with the strongest regulatory ability, regulating 47 mRNAs. Mmu-miR-10a was the most highly expressed in the microRNA-GO-Network and also exerted a strong regulatory ability. The expression profiles of miR-133a and miR-10a were verified by RT-PCR. Conclusions: These results show that, in the early stage, ConA-induced fulminant hepatitis induces a distinct miRNA expression profile. This differential miRNA expression profile may provide pathogenic clues and potential diagnostic and prognostic markers in acute and severe liver disease.http://www.karger.com/Article/FullText/362970Hepatic failuremicroRNAsExpression profileFulminant hepatitis |
| spellingShingle | Hong-Yu Jia Feng Chen Jian-Zhong Chen Shan-Shan Wu Jing Wang Qing-Yi Cao Zhi Chen Hai-Hong Zhu MicroRNA Expression Profiles Related to Early Stage Murine Concanavalin A-Induced Hepatitis Cellular Physiology and Biochemistry Hepatic failure microRNAs Expression profile Fulminant hepatitis |
| title | MicroRNA Expression Profiles Related to Early Stage Murine Concanavalin A-Induced Hepatitis |
| title_full | MicroRNA Expression Profiles Related to Early Stage Murine Concanavalin A-Induced Hepatitis |
| title_fullStr | MicroRNA Expression Profiles Related to Early Stage Murine Concanavalin A-Induced Hepatitis |
| title_full_unstemmed | MicroRNA Expression Profiles Related to Early Stage Murine Concanavalin A-Induced Hepatitis |
| title_short | MicroRNA Expression Profiles Related to Early Stage Murine Concanavalin A-Induced Hepatitis |
| title_sort | microrna expression profiles related to early stage murine concanavalin a induced hepatitis |
| topic | Hepatic failure microRNAs Expression profile Fulminant hepatitis |
| url | http://www.karger.com/Article/FullText/362970 |
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