Association of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma.
BACKGROUND: Airway eosinophilia is a predictor of steroid responsiveness in steroid-naïve asthma. However, the relationship between airway eosinophilia and the expression of FK506-binding protein 51 (FKBP51), a glucocorticoid receptor co-chaperone that plays a role in steroid insensitivity in asthma...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3675168?pdf=render |
| _version_ | 1818466843176730624 |
|---|---|
| author | Tomoko Tajiri Hisako Matsumoto Akio Niimi Isao Ito Tsuyoshi Oguma Hitoshi Nakaji Hideki Inoue Toshiyuki Iwata Tadao Nagasaki Yoshihiro Kanemitsu Guergana Petrova Michiaki Mishima |
| author_facet | Tomoko Tajiri Hisako Matsumoto Akio Niimi Isao Ito Tsuyoshi Oguma Hitoshi Nakaji Hideki Inoue Toshiyuki Iwata Tadao Nagasaki Yoshihiro Kanemitsu Guergana Petrova Michiaki Mishima |
| author_sort | Tomoko Tajiri |
| collection | DOAJ |
| description | BACKGROUND: Airway eosinophilia is a predictor of steroid responsiveness in steroid-naïve asthma. However, the relationship between airway eosinophilia and the expression of FK506-binding protein 51 (FKBP51), a glucocorticoid receptor co-chaperone that plays a role in steroid insensitivity in asthma, remains unknown. OBJECTIVE: To evaluate the relationship between eosinophilic inflammation and FKBP51 expression in sputum cells in asthma. METHODS: The FKBP51 mRNA levels in sputum cells from steroid-naïve patients with asthma (n = 31) and stable asthmatic patients on inhaled corticosteroid (ICS) (n = 28) were cross-sectionally examined using real-time PCR. Associations between FKBP51 levels and clinical indices were analyzed. RESULTS: In steroid-naïve patients, the FKBP51 levels were negatively correlated with eosinophil proportions in blood (r = -0.52) and sputum (r = -0.57), and exhaled nitric oxide levels (r = -0.42) (all p<0.05). No such associations were observed in patients on ICS. In steroid-naïve patients, improvement in forced expiratory volume in one second after ICS initiation was correlated with baseline eosinophil proportions in blood (r = 0.74) and sputum (r = 0.76) and negatively correlated with FKBP51 levels (r = -0.73) (all p<0.0001) (n = 20). Lastly, the FKBP51 levels were the lowest in steroid-naïve asthmatic patients, followed by mild to moderate persistent asthmatic patients on ICS, and the highest in severe persistent asthmatic patients on ICS (p<0.0001). CONCLUSIONS: Lower FKBP51 expression in sputum cells may reflect eosinophilic inflammation and glucocorticoid responsiveness in steroid-naïve asthmatic patients. |
| first_indexed | 2024-04-13T21:01:05Z |
| format | Article |
| id | doaj.art-abd2629ca9b341aab16e8a73a75fdc5c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T21:01:05Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-abd2629ca9b341aab16e8a73a75fdc5c2022-12-22T02:30:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6528410.1371/journal.pone.0065284Association of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma.Tomoko TajiriHisako MatsumotoAkio NiimiIsao ItoTsuyoshi OgumaHitoshi NakajiHideki InoueToshiyuki IwataTadao NagasakiYoshihiro KanemitsuGuergana PetrovaMichiaki MishimaBACKGROUND: Airway eosinophilia is a predictor of steroid responsiveness in steroid-naïve asthma. However, the relationship between airway eosinophilia and the expression of FK506-binding protein 51 (FKBP51), a glucocorticoid receptor co-chaperone that plays a role in steroid insensitivity in asthma, remains unknown. OBJECTIVE: To evaluate the relationship between eosinophilic inflammation and FKBP51 expression in sputum cells in asthma. METHODS: The FKBP51 mRNA levels in sputum cells from steroid-naïve patients with asthma (n = 31) and stable asthmatic patients on inhaled corticosteroid (ICS) (n = 28) were cross-sectionally examined using real-time PCR. Associations between FKBP51 levels and clinical indices were analyzed. RESULTS: In steroid-naïve patients, the FKBP51 levels were negatively correlated with eosinophil proportions in blood (r = -0.52) and sputum (r = -0.57), and exhaled nitric oxide levels (r = -0.42) (all p<0.05). No such associations were observed in patients on ICS. In steroid-naïve patients, improvement in forced expiratory volume in one second after ICS initiation was correlated with baseline eosinophil proportions in blood (r = 0.74) and sputum (r = 0.76) and negatively correlated with FKBP51 levels (r = -0.73) (all p<0.0001) (n = 20). Lastly, the FKBP51 levels were the lowest in steroid-naïve asthmatic patients, followed by mild to moderate persistent asthmatic patients on ICS, and the highest in severe persistent asthmatic patients on ICS (p<0.0001). CONCLUSIONS: Lower FKBP51 expression in sputum cells may reflect eosinophilic inflammation and glucocorticoid responsiveness in steroid-naïve asthmatic patients.http://europepmc.org/articles/PMC3675168?pdf=render |
| spellingShingle | Tomoko Tajiri Hisako Matsumoto Akio Niimi Isao Ito Tsuyoshi Oguma Hitoshi Nakaji Hideki Inoue Toshiyuki Iwata Tadao Nagasaki Yoshihiro Kanemitsu Guergana Petrova Michiaki Mishima Association of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma. PLoS ONE |
| title | Association of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma. |
| title_full | Association of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma. |
| title_fullStr | Association of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma. |
| title_full_unstemmed | Association of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma. |
| title_short | Association of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma. |
| title_sort | association of eosinophilic inflammation with fkbp51 expression in sputum cells in asthma |
| url | http://europepmc.org/articles/PMC3675168?pdf=render |
| work_keys_str_mv | AT tomokotajiri associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT hisakomatsumoto associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT akioniimi associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT isaoito associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT tsuyoshioguma associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT hitoshinakaji associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT hidekiinoue associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT toshiyukiiwata associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT tadaonagasaki associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT yoshihirokanemitsu associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT guerganapetrova associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma AT michiakimishima associationofeosinophilicinflammationwithfkbp51expressioninsputumcellsinasthma |