Insights into Stress-Induced Death Processes during Aging in the Marine Bloom-Forming Dinoflagellate <i>Karenia brevis</i>

Harmful algal blooms (HABs) globally affect marine ecosystems and human health. Significant attention has been paid to understanding the initiation processes of HABs, while much less is known of the mechanisms causing cell demise and bloom decline. Recent evidence reveals that programmed cell death...

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Main Authors: Yida Gao, Deana L. Erdner
Format: Article
Language:English
Published: MDPI AG 2022-12-01
Series:Journal of Marine Science and Engineering
Subjects:
Online Access:https://www.mdpi.com/2077-1312/10/12/1993
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author Yida Gao
Deana L. Erdner
author_facet Yida Gao
Deana L. Erdner
author_sort Yida Gao
collection DOAJ
description Harmful algal blooms (HABs) globally affect marine ecosystems and human health. Significant attention has been paid to understanding the initiation processes of HABs, while much less is known of the mechanisms causing cell demise and bloom decline. Recent evidence reveals that programmed cell death (PCD) can be a possible pathway for HAB termination. However, it is still not clear how PCD expression varies with the growth of marine phytoplankton. In order to characterize how susceptibility to stress-induced cell death changes with the growth of marine harmful algae, we examined the prevalence of PCD markers during 48 h after the addition of H<sub>2</sub>O<sub>2</sub> in dinoflagellate <i>Karenia brevis</i> cultures at the early-log, mid-log and stationary phase. The results show that acute susceptibility to cell death (before time 0.5 h) increased as cultures aged, reflecting a chronological decrease in stress acclimation abilities. However, the youngest cultures showed strong PCD expression and the fastest overall rate of cell loss within the first 24 h, hypothesized to result from the cell-density-dependent H<sub>2</sub>O<sub>2</sub> detoxifying process. This research highlights the existence of PCD in HAB species and that aging marine phytoplankton are more susceptible to exogenous stress, which agrees with previous observations of significant PCD during bloom decline.
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spelling doaj.art-abd76ea2254d468db86fb2ba3a72717e2023-11-24T15:57:49ZengMDPI AGJournal of Marine Science and Engineering2077-13122022-12-011012199310.3390/jmse10121993Insights into Stress-Induced Death Processes during Aging in the Marine Bloom-Forming Dinoflagellate <i>Karenia brevis</i>Yida Gao0Deana L. Erdner1Marine Science Institute, The University of Texas at Austin, Port Aransas, TX 78373, USAMarine Science Institute, The University of Texas at Austin, Port Aransas, TX 78373, USAHarmful algal blooms (HABs) globally affect marine ecosystems and human health. Significant attention has been paid to understanding the initiation processes of HABs, while much less is known of the mechanisms causing cell demise and bloom decline. Recent evidence reveals that programmed cell death (PCD) can be a possible pathway for HAB termination. However, it is still not clear how PCD expression varies with the growth of marine phytoplankton. In order to characterize how susceptibility to stress-induced cell death changes with the growth of marine harmful algae, we examined the prevalence of PCD markers during 48 h after the addition of H<sub>2</sub>O<sub>2</sub> in dinoflagellate <i>Karenia brevis</i> cultures at the early-log, mid-log and stationary phase. The results show that acute susceptibility to cell death (before time 0.5 h) increased as cultures aged, reflecting a chronological decrease in stress acclimation abilities. However, the youngest cultures showed strong PCD expression and the fastest overall rate of cell loss within the first 24 h, hypothesized to result from the cell-density-dependent H<sub>2</sub>O<sub>2</sub> detoxifying process. This research highlights the existence of PCD in HAB species and that aging marine phytoplankton are more susceptible to exogenous stress, which agrees with previous observations of significant PCD during bloom decline.https://www.mdpi.com/2077-1312/10/12/1993harmful algal bloomstressprogrammed cell deathgrowth stages
spellingShingle Yida Gao
Deana L. Erdner
Insights into Stress-Induced Death Processes during Aging in the Marine Bloom-Forming Dinoflagellate <i>Karenia brevis</i>
Journal of Marine Science and Engineering
harmful algal bloom
stress
programmed cell death
growth stages
title Insights into Stress-Induced Death Processes during Aging in the Marine Bloom-Forming Dinoflagellate <i>Karenia brevis</i>
title_full Insights into Stress-Induced Death Processes during Aging in the Marine Bloom-Forming Dinoflagellate <i>Karenia brevis</i>
title_fullStr Insights into Stress-Induced Death Processes during Aging in the Marine Bloom-Forming Dinoflagellate <i>Karenia brevis</i>
title_full_unstemmed Insights into Stress-Induced Death Processes during Aging in the Marine Bloom-Forming Dinoflagellate <i>Karenia brevis</i>
title_short Insights into Stress-Induced Death Processes during Aging in the Marine Bloom-Forming Dinoflagellate <i>Karenia brevis</i>
title_sort insights into stress induced death processes during aging in the marine bloom forming dinoflagellate i karenia brevis i
topic harmful algal bloom
stress
programmed cell death
growth stages
url https://www.mdpi.com/2077-1312/10/12/1993
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