Striatal Isolated from <i>Cyathus striatus</i> Extracts Induces Apoptosis in Human Pancreatic Cancer Cells
The aim of the present study was to identify the structure of active compounds in <i>Cyathus stratus</i> that previously demonstrated anti-pancreatic cancer activity. The active compounds were purified from a crude extract by a series of RP-18 preparative chromatography using homemade oc...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/9/2746 |
| _version_ | 1797503756155224064 |
|---|---|
| author | Fuad Fares Soliman Khatib Jacob Vaya Lital Sharvit Einav Eizenberg Solomon Wasser |
| author_facet | Fuad Fares Soliman Khatib Jacob Vaya Lital Sharvit Einav Eizenberg Solomon Wasser |
| author_sort | Fuad Fares |
| collection | DOAJ |
| description | The aim of the present study was to identify the structure of active compounds in <i>Cyathus stratus</i> that previously demonstrated anti-pancreatic cancer activity. The active compounds were purified from a crude extract by a series of RP-18 preparative chromatography using homemade octadecyl silica gel column. HPLC injection of the crude extract revealed a chromatogram with three main peaks with retention times (RT) 15.6, 18.2, and 22.5 min. Each fraction that exhibited promising activity in vitro was further separated using various available chromatographic techniques. The purified compound with the ultimate anti-cancer activity appeared at RT of 15.8 in the HPLC chromatogram with more than 90% purity. The main peak at the mass spectra appeared at <i>m</i>/<i>z</i> = 446.2304 with the calculated molecular formula of C<sub>25</sub>H<sub>34</sub>O<sub>7</sub>. One- and two-dimensional NMR analyses indicated that the structure of the active molecule (peak 15.8 min in HPLC) was identified as striatal C. Exposure of human pancreatic cancer cells to purified striatal C resulted in induction of apoptosis. Further studies are needed in order to develop a method for the synthesis of striatal in order to use it in clinical studies for treatment of cancer. |
| first_indexed | 2024-03-10T03:54:52Z |
| format | Article |
| id | doaj.art-abd7c1607fb34d8787a53bcc3d3e1f21 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:54:52Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-abd7c1607fb34d8787a53bcc3d3e1f212023-11-23T08:48:58ZengMDPI AGMolecules1420-30492022-04-01279274610.3390/molecules27092746Striatal Isolated from <i>Cyathus striatus</i> Extracts Induces Apoptosis in Human Pancreatic Cancer CellsFuad Fares0Soliman Khatib1Jacob Vaya2Lital Sharvit3Einav Eizenberg4Solomon Wasser5Department of Human Biology, Faculty of Natural Sciences, University of Haifa, Haifa 3498838, IsraelLaboratory of Natural Compounds and Analytical Chemistry, MIGAL Galilee Research Institute, Kiryat Shmona 11016, IsraelLaboratory of Natural Compounds and Analytical Chemistry, MIGAL Galilee Research Institute, Kiryat Shmona 11016, IsraelDepartment of Human Biology, Faculty of Natural Sciences, University of Haifa, Haifa 3498838, IsraelDepartment of Human Biology, Faculty of Natural Sciences, University of Haifa, Haifa 3498838, IsraelInstitute of Evolution and Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, Haifa 3498838, IsraelThe aim of the present study was to identify the structure of active compounds in <i>Cyathus stratus</i> that previously demonstrated anti-pancreatic cancer activity. The active compounds were purified from a crude extract by a series of RP-18 preparative chromatography using homemade octadecyl silica gel column. HPLC injection of the crude extract revealed a chromatogram with three main peaks with retention times (RT) 15.6, 18.2, and 22.5 min. Each fraction that exhibited promising activity in vitro was further separated using various available chromatographic techniques. The purified compound with the ultimate anti-cancer activity appeared at RT of 15.8 in the HPLC chromatogram with more than 90% purity. The main peak at the mass spectra appeared at <i>m</i>/<i>z</i> = 446.2304 with the calculated molecular formula of C<sub>25</sub>H<sub>34</sub>O<sub>7</sub>. One- and two-dimensional NMR analyses indicated that the structure of the active molecule (peak 15.8 min in HPLC) was identified as striatal C. Exposure of human pancreatic cancer cells to purified striatal C resulted in induction of apoptosis. Further studies are needed in order to develop a method for the synthesis of striatal in order to use it in clinical studies for treatment of cancer.https://www.mdpi.com/1420-3049/27/9/2746medicinal mushroom<i>Cyathus striatus</i>striatalpancreatic cancerapoptosis |
| spellingShingle | Fuad Fares Soliman Khatib Jacob Vaya Lital Sharvit Einav Eizenberg Solomon Wasser Striatal Isolated from <i>Cyathus striatus</i> Extracts Induces Apoptosis in Human Pancreatic Cancer Cells Molecules medicinal mushroom <i>Cyathus striatus</i> striatal pancreatic cancer apoptosis |
| title | Striatal Isolated from <i>Cyathus striatus</i> Extracts Induces Apoptosis in Human Pancreatic Cancer Cells |
| title_full | Striatal Isolated from <i>Cyathus striatus</i> Extracts Induces Apoptosis in Human Pancreatic Cancer Cells |
| title_fullStr | Striatal Isolated from <i>Cyathus striatus</i> Extracts Induces Apoptosis in Human Pancreatic Cancer Cells |
| title_full_unstemmed | Striatal Isolated from <i>Cyathus striatus</i> Extracts Induces Apoptosis in Human Pancreatic Cancer Cells |
| title_short | Striatal Isolated from <i>Cyathus striatus</i> Extracts Induces Apoptosis in Human Pancreatic Cancer Cells |
| title_sort | striatal isolated from i cyathus striatus i extracts induces apoptosis in human pancreatic cancer cells |
| topic | medicinal mushroom <i>Cyathus striatus</i> striatal pancreatic cancer apoptosis |
| url | https://www.mdpi.com/1420-3049/27/9/2746 |
| work_keys_str_mv | AT fuadfares striatalisolatedfromicyathusstriatusiextractsinducesapoptosisinhumanpancreaticcancercells AT solimankhatib striatalisolatedfromicyathusstriatusiextractsinducesapoptosisinhumanpancreaticcancercells AT jacobvaya striatalisolatedfromicyathusstriatusiextractsinducesapoptosisinhumanpancreaticcancercells AT litalsharvit striatalisolatedfromicyathusstriatusiextractsinducesapoptosisinhumanpancreaticcancercells AT einaveizenberg striatalisolatedfromicyathusstriatusiextractsinducesapoptosisinhumanpancreaticcancercells AT solomonwasser striatalisolatedfromicyathusstriatusiextractsinducesapoptosisinhumanpancreaticcancercells |