Calibration of Spectral Imaging Devices With Oxygenation-Controlled Phantoms: Introducing a Simple Gel-Based Hemoglobin Model
An optical phantom model with stable oxygen saturation (StO2) and optical properties (OPs) is the key for quick and accurate calibration of spectral imaging devices. Toward this goal, we have developed gel-based phantoms using Agarose, polystyrene microsphere, purified hemoglobin, and yeast. Theoret...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-11-01
|
| Series: | Frontiers in Physics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphy.2019.00192/full |
| _version_ | 1819099450104086528 |
|---|---|
| author | V. N. Du Le Myla Manser Sunny Gurm Ben Wagner Joseph E. Hayward Qiyin Fang Qiyin Fang |
| author_facet | V. N. Du Le Myla Manser Sunny Gurm Ben Wagner Joseph E. Hayward Qiyin Fang Qiyin Fang |
| author_sort | V. N. Du Le |
| collection | DOAJ |
| description | An optical phantom model with stable oxygen saturation (StO2) and optical properties (OPs) is the key for quick and accurate calibration of spectral imaging devices. Toward this goal, we have developed gel-based phantoms using Agarose, polystyrene microsphere, purified hemoglobin, and yeast. Theoretical values of OPs were calculated by applying Beer Lambert law and Mie theory whereas a robust fitting algorithm was used to extract StO2. Stability of OPs and StO2 in the phantoms was validated experimentally over a period of 2 weeks and the effect of pre-heating at 65°C degrees on StO2 was observed. Multi-spectral imaging (750, 780, 850, and 945 nm) of the phantoms were also performed on the FDA-approved commercial system to demonstrate the utility of the phantoms. |
| first_indexed | 2024-12-22T00:47:04Z |
| format | Article |
| id | doaj.art-abd919046c5a4828bfb8a958346197a4 |
| institution | Directory Open Access Journal |
| issn | 2296-424X |
| language | English |
| last_indexed | 2024-12-22T00:47:04Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physics |
| spelling | doaj.art-abd919046c5a4828bfb8a958346197a42022-12-21T18:44:31ZengFrontiers Media S.A.Frontiers in Physics2296-424X2019-11-01710.3389/fphy.2019.00192488791Calibration of Spectral Imaging Devices With Oxygenation-Controlled Phantoms: Introducing a Simple Gel-Based Hemoglobin ModelV. N. Du Le0Myla Manser1Sunny Gurm2Ben Wagner3Joseph E. Hayward4Qiyin Fang5Qiyin Fang6Department of Biomedical Engineering, University of California, Davis, Davis, CA, United StatesEngineering Physics, McMaster University, Hamilton, ON, CanadaChristie Medical Holdings, Kitchener, ON, CanadaChristie Medical Holdings, Kitchener, ON, CanadaSchool of Biomedical Engineering, McMaster University, Hamilton, ON, CanadaEngineering Physics, McMaster University, Hamilton, ON, CanadaSchool of Biomedical Engineering, McMaster University, Hamilton, ON, CanadaAn optical phantom model with stable oxygen saturation (StO2) and optical properties (OPs) is the key for quick and accurate calibration of spectral imaging devices. Toward this goal, we have developed gel-based phantoms using Agarose, polystyrene microsphere, purified hemoglobin, and yeast. Theoretical values of OPs were calculated by applying Beer Lambert law and Mie theory whereas a robust fitting algorithm was used to extract StO2. Stability of OPs and StO2 in the phantoms was validated experimentally over a period of 2 weeks and the effect of pre-heating at 65°C degrees on StO2 was observed. Multi-spectral imaging (750, 780, 850, and 945 nm) of the phantoms were also performed on the FDA-approved commercial system to demonstrate the utility of the phantoms.https://www.frontiersin.org/article/10.3389/fphy.2019.00192/fullmulti-spectral imaging (MSI)hemoglobinoptical propertiesoxygen saturationoptical phantomscattering |
| spellingShingle | V. N. Du Le Myla Manser Sunny Gurm Ben Wagner Joseph E. Hayward Qiyin Fang Qiyin Fang Calibration of Spectral Imaging Devices With Oxygenation-Controlled Phantoms: Introducing a Simple Gel-Based Hemoglobin Model Frontiers in Physics multi-spectral imaging (MSI) hemoglobin optical properties oxygen saturation optical phantom scattering |
| title | Calibration of Spectral Imaging Devices With Oxygenation-Controlled Phantoms: Introducing a Simple Gel-Based Hemoglobin Model |
| title_full | Calibration of Spectral Imaging Devices With Oxygenation-Controlled Phantoms: Introducing a Simple Gel-Based Hemoglobin Model |
| title_fullStr | Calibration of Spectral Imaging Devices With Oxygenation-Controlled Phantoms: Introducing a Simple Gel-Based Hemoglobin Model |
| title_full_unstemmed | Calibration of Spectral Imaging Devices With Oxygenation-Controlled Phantoms: Introducing a Simple Gel-Based Hemoglobin Model |
| title_short | Calibration of Spectral Imaging Devices With Oxygenation-Controlled Phantoms: Introducing a Simple Gel-Based Hemoglobin Model |
| title_sort | calibration of spectral imaging devices with oxygenation controlled phantoms introducing a simple gel based hemoglobin model |
| topic | multi-spectral imaging (MSI) hemoglobin optical properties oxygen saturation optical phantom scattering |
| url | https://www.frontiersin.org/article/10.3389/fphy.2019.00192/full |
| work_keys_str_mv | AT vndule calibrationofspectralimagingdeviceswithoxygenationcontrolledphantomsintroducingasimplegelbasedhemoglobinmodel AT mylamanser calibrationofspectralimagingdeviceswithoxygenationcontrolledphantomsintroducingasimplegelbasedhemoglobinmodel AT sunnygurm calibrationofspectralimagingdeviceswithoxygenationcontrolledphantomsintroducingasimplegelbasedhemoglobinmodel AT benwagner calibrationofspectralimagingdeviceswithoxygenationcontrolledphantomsintroducingasimplegelbasedhemoglobinmodel AT josephehayward calibrationofspectralimagingdeviceswithoxygenationcontrolledphantomsintroducingasimplegelbasedhemoglobinmodel AT qiyinfang calibrationofspectralimagingdeviceswithoxygenationcontrolledphantomsintroducingasimplegelbasedhemoglobinmodel AT qiyinfang calibrationofspectralimagingdeviceswithoxygenationcontrolledphantomsintroducingasimplegelbasedhemoglobinmodel |