The Vibriolysin-Like Protease VnpA and the Collagenase ColA Are Required for Full Virulence of the Bivalve Mollusks Pathogen <i>Vibrio neptunius</i>
<i>Vibrio neptunius</i> is an important pathogen of bivalve mollusks worldwide. Several metalloproteases have been described as virulence factors in species of <i>Vibrio</i> that are pathogenic to bivalves, but little is known about the contribution of these potential virulen...
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MDPI AG
2021-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/4/391 |
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| author | Fabián Galvis Juan L. Barja Manuel L. Lemos Miguel Balado |
| author_facet | Fabián Galvis Juan L. Barja Manuel L. Lemos Miguel Balado |
| author_sort | Fabián Galvis |
| collection | DOAJ |
| description | <i>Vibrio neptunius</i> is an important pathogen of bivalve mollusks worldwide. Several metalloproteases have been described as virulence factors in species of <i>Vibrio</i> that are pathogenic to bivalves, but little is known about the contribution of these potential virulence factors to <i>Vibrio neptunius</i> pathogenesis. In silico analysis of the genome of <i>V. neptunius</i> strain PP-145.98 led to the identification of two hitherto uncharacterized chromosomal loci encoding a probable vibriolysin-like metalloprotease and a putative collagenase, which were designated VnpA and ColA, respectively. Single defective mutants of each gene were obtained in <i>V. neptunius</i> PP-145.98, and the phospholipase, esterase and collagenase activities were studied and compared with those of the wild-type strain. The results showed that the single inactivation of <i>vnpA</i> resulted in a 3-fold reduction in phospholipase/esterase activity. Inactivation of <i>colA</i> reduced the collagenase activity by 50%. Finally, infection challenges performed in oyster larvae showed that Δ<i>vnpA</i> and Δ<i>colA</i>—single mutant strains of <i>V. neptunius</i>—are between 2–3-fold less virulent than the wild-type strain. Thus, the present work demonstrates that the production of both VnpA and ColA is required for the full virulence of the bivalve pathogen <i>V. neptunius</i>. |
| first_indexed | 2024-03-10T12:35:05Z |
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| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T12:35:05Z |
| publishDate | 2021-04-01 |
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| spelling | doaj.art-abd97d92ff74434eb034100d105e049c2023-11-21T14:21:09ZengMDPI AGAntibiotics2079-63822021-04-0110439110.3390/antibiotics10040391The Vibriolysin-Like Protease VnpA and the Collagenase ColA Are Required for Full Virulence of the Bivalve Mollusks Pathogen <i>Vibrio neptunius</i>Fabián Galvis0Juan L. Barja1Manuel L. Lemos2Miguel Balado3Departamento de Microbiología y Parasitología, Instituto de Acuicultura y CIBUS-Facultad de Biología, Universidade de Santiago de Compostela, 15705 Santiago de Compostela, A Coruña, SpainDepartamento de Microbiología y Parasitología, Instituto de Acuicultura y CIBUS-Facultad de Biología, Universidade de Santiago de Compostela, 15705 Santiago de Compostela, A Coruña, SpainDepartamento de Microbiología y Parasitología, Instituto de Acuicultura y CIBUS-Facultad de Biología, Universidade de Santiago de Compostela, 15705 Santiago de Compostela, A Coruña, SpainDepartamento de Microbiología y Parasitología, Instituto de Acuicultura y CIBUS-Facultad de Biología, Universidade de Santiago de Compostela, 15705 Santiago de Compostela, A Coruña, Spain<i>Vibrio neptunius</i> is an important pathogen of bivalve mollusks worldwide. Several metalloproteases have been described as virulence factors in species of <i>Vibrio</i> that are pathogenic to bivalves, but little is known about the contribution of these potential virulence factors to <i>Vibrio neptunius</i> pathogenesis. In silico analysis of the genome of <i>V. neptunius</i> strain PP-145.98 led to the identification of two hitherto uncharacterized chromosomal loci encoding a probable vibriolysin-like metalloprotease and a putative collagenase, which were designated VnpA and ColA, respectively. Single defective mutants of each gene were obtained in <i>V. neptunius</i> PP-145.98, and the phospholipase, esterase and collagenase activities were studied and compared with those of the wild-type strain. The results showed that the single inactivation of <i>vnpA</i> resulted in a 3-fold reduction in phospholipase/esterase activity. Inactivation of <i>colA</i> reduced the collagenase activity by 50%. Finally, infection challenges performed in oyster larvae showed that Δ<i>vnpA</i> and Δ<i>colA</i>—single mutant strains of <i>V. neptunius</i>—are between 2–3-fold less virulent than the wild-type strain. Thus, the present work demonstrates that the production of both VnpA and ColA is required for the full virulence of the bivalve pathogen <i>V. neptunius</i>.https://www.mdpi.com/2079-6382/10/4/391<i>Vibrio neptunius</i>virulencebivalve mollusk pathogenmetalloproteasecollagenaseVnpA |
| spellingShingle | Fabián Galvis Juan L. Barja Manuel L. Lemos Miguel Balado The Vibriolysin-Like Protease VnpA and the Collagenase ColA Are Required for Full Virulence of the Bivalve Mollusks Pathogen <i>Vibrio neptunius</i> Antibiotics <i>Vibrio neptunius</i> virulence bivalve mollusk pathogen metalloprotease collagenase VnpA |
| title | The Vibriolysin-Like Protease VnpA and the Collagenase ColA Are Required for Full Virulence of the Bivalve Mollusks Pathogen <i>Vibrio neptunius</i> |
| title_full | The Vibriolysin-Like Protease VnpA and the Collagenase ColA Are Required for Full Virulence of the Bivalve Mollusks Pathogen <i>Vibrio neptunius</i> |
| title_fullStr | The Vibriolysin-Like Protease VnpA and the Collagenase ColA Are Required for Full Virulence of the Bivalve Mollusks Pathogen <i>Vibrio neptunius</i> |
| title_full_unstemmed | The Vibriolysin-Like Protease VnpA and the Collagenase ColA Are Required for Full Virulence of the Bivalve Mollusks Pathogen <i>Vibrio neptunius</i> |
| title_short | The Vibriolysin-Like Protease VnpA and the Collagenase ColA Are Required for Full Virulence of the Bivalve Mollusks Pathogen <i>Vibrio neptunius</i> |
| title_sort | vibriolysin like protease vnpa and the collagenase cola are required for full virulence of the bivalve mollusks pathogen i vibrio neptunius i |
| topic | <i>Vibrio neptunius</i> virulence bivalve mollusk pathogen metalloprotease collagenase VnpA |
| url | https://www.mdpi.com/2079-6382/10/4/391 |
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