Polymorphism of microsatellite loci OarCP549, CSRD247, FCB20 and MAF65 in sheep
Rostov Region is one of the most important regions of sheep breeding in Russia. The Salsk breed of sheep is created as a result of many years work of breeders in the Rostov region. Studies on the genetic diversity of domestic breeds of sheep are a prerequisite for their conservation, further improve...
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| Format: | Article |
| Language: | Russian |
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Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
2017-10-01
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| Series: | Аграрная наука Евро-Северо-Востока |
| Subjects: | |
| Online Access: | https://www.agronauka-sv.ru/jour/article/view/162 |
| _version_ | 1797290352933076992 |
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| author | N. V. Shirokova L. V. Getmantseva Yu. A. Kolosov N. F. Bakoev T. E. Deniskova S. Yu. Bakoev V. V. Volkova T. S. Romanets |
| author_facet | N. V. Shirokova L. V. Getmantseva Yu. A. Kolosov N. F. Bakoev T. E. Deniskova S. Yu. Bakoev V. V. Volkova T. S. Romanets |
| author_sort | N. V. Shirokova |
| collection | DOAJ |
| description | Rostov Region is one of the most important regions of sheep breeding in Russia. The Salsk breed of sheep is created as a result of many years work of breeders in the Rostov region. Studies on the genetic diversity of domestic breeds of sheep are a prerequisite for their conservation, further improvement and use for the needs of the modern agro-industrial complex of the Russian Federation. The aim of the work is to study polymorphism of microsatellite loci OarCP549, CSRD247, FCB20, and MAF65 in sheep of Salsk breed. Studies were carried out on sheep of the Salsk breed (n = 95, PZ «Belozernoe», Rostov Region). Analysis of microsatellites was performed on sixteen-capillary genetic analyzer ABI3130xl Genetic Analyzer. To estimate polymorphism of loci it was taken into account: the Number of Alleles per locus (NA); Observed (Ho) and the Expected (He) Heterozygosity, the Effective Number of Alleles per locus (Ae). The highest Number of Alleles (NA = 17) and the Effective Number of Alleles (Ae = 7) was determined from the locus OarCP49 in the sheep of the Salsk breed. At other loci, the Number of Alleles was 15, 12 and 9, and the Effective Number of Alleles was 5.56, 5.33 and 3.85 respectively for CSRD247, FCB20 and MAF65. The heterozygote deficiency in the population is noted at the loci CSRD247 and MAF65. Average index of fixation for four loci was 0.094. The results of the work showed the polymorphism of microsatellite loci OarCP49, CSRD247, FCB20 and MAF65 and the expediency of their inclusion in the panel of DNA markers for assessing the genetic diversity and authenticity of the origin for the sheep of Salsk breed. |
| first_indexed | 2024-03-07T19:20:19Z |
| format | Article |
| id | doaj.art-abdc111756fd4f7b9f459cdce67b1fa6 |
| institution | Directory Open Access Journal |
| issn | 2072-9081 2500-1396 |
| language | Russian |
| last_indexed | 2024-03-07T19:20:19Z |
| publishDate | 2017-10-01 |
| publisher | Federal Agricultural Research Center of the North-East named N.V. Rudnitsky |
| record_format | Article |
| series | Аграрная наука Евро-Северо-Востока |
| spelling | doaj.art-abdc111756fd4f7b9f459cdce67b1fa62024-02-29T11:31:10ZrusFederal Agricultural Research Center of the North-East named N.V. RudnitskyАграрная наука Евро-Северо-Востока2072-90812500-13962017-10-01055762161Polymorphism of microsatellite loci OarCP549, CSRD247, FCB20 and MAF65 in sheepN. V. Shirokova0L. V. Getmantseva1Yu. A. Kolosov2N. F. Bakoev3T. E. Deniskova4S. Yu. Bakoev5V. V. Volkova6T. S. Romanets7Donskoj State Agrarian UniversityDonskoj State Agrarian UniversityDonskoj State Agrarian UniversityDonskoj State Agrarian UniversityAll-Russian Institute of Animal HusbandryDonskoj State Agrarian UniversityAll-Russian Institute of Animal HusbandryDonskoj State Agrarian UniversityRostov Region is one of the most important regions of sheep breeding in Russia. The Salsk breed of sheep is created as a result of many years work of breeders in the Rostov region. Studies on the genetic diversity of domestic breeds of sheep are a prerequisite for their conservation, further improvement and use for the needs of the modern agro-industrial complex of the Russian Federation. The aim of the work is to study polymorphism of microsatellite loci OarCP549, CSRD247, FCB20, and MAF65 in sheep of Salsk breed. Studies were carried out on sheep of the Salsk breed (n = 95, PZ «Belozernoe», Rostov Region). Analysis of microsatellites was performed on sixteen-capillary genetic analyzer ABI3130xl Genetic Analyzer. To estimate polymorphism of loci it was taken into account: the Number of Alleles per locus (NA); Observed (Ho) and the Expected (He) Heterozygosity, the Effective Number of Alleles per locus (Ae). The highest Number of Alleles (NA = 17) and the Effective Number of Alleles (Ae = 7) was determined from the locus OarCP49 in the sheep of the Salsk breed. At other loci, the Number of Alleles was 15, 12 and 9, and the Effective Number of Alleles was 5.56, 5.33 and 3.85 respectively for CSRD247, FCB20 and MAF65. The heterozygote deficiency in the population is noted at the loci CSRD247 and MAF65. Average index of fixation for four loci was 0.094. The results of the work showed the polymorphism of microsatellite loci OarCP49, CSRD247, FCB20 and MAF65 and the expediency of their inclusion in the panel of DNA markers for assessing the genetic diversity and authenticity of the origin for the sheep of Salsk breed.https://www.agronauka-sv.ru/jour/article/view/162oarcp49csrd247fcb20 и maf65genetic diversitymicrosatellitespolymorphismsheepsalsk breedoarcp49csrd247fcb20 and maf65 |
| spellingShingle | N. V. Shirokova L. V. Getmantseva Yu. A. Kolosov N. F. Bakoev T. E. Deniskova S. Yu. Bakoev V. V. Volkova T. S. Romanets Polymorphism of microsatellite loci OarCP549, CSRD247, FCB20 and MAF65 in sheep Аграрная наука Евро-Северо-Востока oarcp49 csrd247 fcb20 и maf65 genetic diversity microsatellites polymorphism sheep salsk breed oarcp49 csrd247 fcb20 and maf65 |
| title | Polymorphism of microsatellite loci OarCP549, CSRD247, FCB20 and MAF65 in sheep |
| title_full | Polymorphism of microsatellite loci OarCP549, CSRD247, FCB20 and MAF65 in sheep |
| title_fullStr | Polymorphism of microsatellite loci OarCP549, CSRD247, FCB20 and MAF65 in sheep |
| title_full_unstemmed | Polymorphism of microsatellite loci OarCP549, CSRD247, FCB20 and MAF65 in sheep |
| title_short | Polymorphism of microsatellite loci OarCP549, CSRD247, FCB20 and MAF65 in sheep |
| title_sort | polymorphism of microsatellite loci oarcp549 csrd247 fcb20 and maf65 in sheep |
| topic | oarcp49 csrd247 fcb20 и maf65 genetic diversity microsatellites polymorphism sheep salsk breed oarcp49 csrd247 fcb20 and maf65 |
| url | https://www.agronauka-sv.ru/jour/article/view/162 |
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