Fatty acid and peptide profiles in plasma membrane and membrane rafts of PUFA supplemented RAW264.7 macrophages.
The eukaryotic cell membrane possesses numerous complex functions, which are essential for life. At this, the composition and the structure of the lipid bilayer are of particular importance. Polyunsaturated fatty acids may modulate the physical properties of biological membranes via alteration of me...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21887374/?tool=EBI |
| _version_ | 1818388430014382080 |
|---|---|
| author | Julia Schumann Alexander Leichtle Joachim Thiery Herbert Fuhrmann |
| author_facet | Julia Schumann Alexander Leichtle Joachim Thiery Herbert Fuhrmann |
| author_sort | Julia Schumann |
| collection | DOAJ |
| description | The eukaryotic cell membrane possesses numerous complex functions, which are essential for life. At this, the composition and the structure of the lipid bilayer are of particular importance. Polyunsaturated fatty acids may modulate the physical properties of biological membranes via alteration of membrane lipid composition affecting numerous physiological processes, e.g. in the immune system. In this systematic study we present fatty acid and peptide profiles of cell membrane and membrane rafts of murine macrophages that have been supplemented with saturated fatty acids as well as PUFAs from the n-3, the n-6 and the n-9 family. Using fatty acid composition analysis and mass spectrometry-based peptidome profiling we found that PUFAs from both the n-3 and the n-6 family have an impact on lipid and protein composition of plasma membrane and membrane rafts in a similar manner. In addition, we found a relation between the number of bis-allyl-methylene positions of the PUFA added and the unsaturation index of plasma membrane as well as membrane rafts of supplemented cells. With regard to the proposed significance of lipid microdomains for disease development and treatment our study will help to achieve a targeted dietary modulation of immune cell lipid bilayers. |
| first_indexed | 2024-12-14T04:25:42Z |
| format | Article |
| id | doaj.art-abde363c10da42f7b8d0096bce5c7b69 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T04:25:42Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-abde363c10da42f7b8d0096bce5c7b692022-12-21T23:17:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2406610.1371/journal.pone.0024066Fatty acid and peptide profiles in plasma membrane and membrane rafts of PUFA supplemented RAW264.7 macrophages.Julia SchumannAlexander LeichtleJoachim ThieryHerbert FuhrmannThe eukaryotic cell membrane possesses numerous complex functions, which are essential for life. At this, the composition and the structure of the lipid bilayer are of particular importance. Polyunsaturated fatty acids may modulate the physical properties of biological membranes via alteration of membrane lipid composition affecting numerous physiological processes, e.g. in the immune system. In this systematic study we present fatty acid and peptide profiles of cell membrane and membrane rafts of murine macrophages that have been supplemented with saturated fatty acids as well as PUFAs from the n-3, the n-6 and the n-9 family. Using fatty acid composition analysis and mass spectrometry-based peptidome profiling we found that PUFAs from both the n-3 and the n-6 family have an impact on lipid and protein composition of plasma membrane and membrane rafts in a similar manner. In addition, we found a relation between the number of bis-allyl-methylene positions of the PUFA added and the unsaturation index of plasma membrane as well as membrane rafts of supplemented cells. With regard to the proposed significance of lipid microdomains for disease development and treatment our study will help to achieve a targeted dietary modulation of immune cell lipid bilayers.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21887374/?tool=EBI |
| spellingShingle | Julia Schumann Alexander Leichtle Joachim Thiery Herbert Fuhrmann Fatty acid and peptide profiles in plasma membrane and membrane rafts of PUFA supplemented RAW264.7 macrophages. PLoS ONE |
| title | Fatty acid and peptide profiles in plasma membrane and membrane rafts of PUFA supplemented RAW264.7 macrophages. |
| title_full | Fatty acid and peptide profiles in plasma membrane and membrane rafts of PUFA supplemented RAW264.7 macrophages. |
| title_fullStr | Fatty acid and peptide profiles in plasma membrane and membrane rafts of PUFA supplemented RAW264.7 macrophages. |
| title_full_unstemmed | Fatty acid and peptide profiles in plasma membrane and membrane rafts of PUFA supplemented RAW264.7 macrophages. |
| title_short | Fatty acid and peptide profiles in plasma membrane and membrane rafts of PUFA supplemented RAW264.7 macrophages. |
| title_sort | fatty acid and peptide profiles in plasma membrane and membrane rafts of pufa supplemented raw264 7 macrophages |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21887374/?tool=EBI |
| work_keys_str_mv | AT juliaschumann fattyacidandpeptideprofilesinplasmamembraneandmembraneraftsofpufasupplementedraw2647macrophages AT alexanderleichtle fattyacidandpeptideprofilesinplasmamembraneandmembraneraftsofpufasupplementedraw2647macrophages AT joachimthiery fattyacidandpeptideprofilesinplasmamembraneandmembraneraftsofpufasupplementedraw2647macrophages AT herbertfuhrmann fattyacidandpeptideprofilesinplasmamembraneandmembraneraftsofpufasupplementedraw2647macrophages |