High temperature and humidity in the environment disrupt bile acid metabolism, the gut microbiome, and GLP-1 secretion in mice
Abstract High temperature and humidity in the environment are known to be associated with discomfort and disease, yet the underlying mechanisms remain unclear. We observed a decrease in plasma glucagon-like peptide-1 levels in response to high-temperature and humidity conditions. Through 16S rRNA ge...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-06158-w |
| _version_ | 1797199282601721856 |
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| author | Song Chen Zongren Hu Jianbang Tang Haipeng Zhu Yuhua Zheng Jiedong Xiao Youhua Xu Yao Wang Yi Luo Xiaoying Mo Yalan Wu Jianwen Guo Yongliang Zhang Huanhuan Luo |
| author_facet | Song Chen Zongren Hu Jianbang Tang Haipeng Zhu Yuhua Zheng Jiedong Xiao Youhua Xu Yao Wang Yi Luo Xiaoying Mo Yalan Wu Jianwen Guo Yongliang Zhang Huanhuan Luo |
| author_sort | Song Chen |
| collection | DOAJ |
| description | Abstract High temperature and humidity in the environment are known to be associated with discomfort and disease, yet the underlying mechanisms remain unclear. We observed a decrease in plasma glucagon-like peptide-1 levels in response to high-temperature and humidity conditions. Through 16S rRNA gene sequencing, alterations in the gut microbiota composition were identified following exposure to high temperature and humidity conditions. Notably, changes in the gut microbiota have been implicated in bile acid synthesis. Further analysis revealed a decrease in lithocholic acid levels in high-temperature and humidity conditions. Subsequent in vitro experiments demonstrated that lithocholic acid increases glucagon-like peptide-1 secretion in NCI-H716 cells. Proteomic analysis indicated upregulation of farnesoid X receptor expression in the ileum. In vitro experiments revealed that the combination of lithocholic acid with farnesoid X receptor inhibitors resulted in a significant increase in GLP-1 levels compared to lithocholic acid alone. In this study, we elucidate the mechanism by which reduced lithocholic acid suppresses glucagon-like peptide 1 via farnesoid X receptor activation under high-temperature and humidity condition. |
| first_indexed | 2024-04-24T07:13:17Z |
| format | Article |
| id | doaj.art-abde9e7190424ee49322411ff12596b7 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-04-24T07:13:17Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-abde9e7190424ee49322411ff12596b72024-04-21T11:27:25ZengNature PortfolioCommunications Biology2399-36422024-04-017111310.1038/s42003-024-06158-wHigh temperature and humidity in the environment disrupt bile acid metabolism, the gut microbiome, and GLP-1 secretion in miceSong Chen0Zongren Hu1Jianbang Tang2Haipeng Zhu3Yuhua Zheng4Jiedong Xiao5Youhua Xu6Yao Wang7Yi Luo8Xiaoying Mo9Yalan Wu10Jianwen Guo11Yongliang Zhang12Huanhuan Luo13Science and Technology Innovation Center, Guangzhou University of Chinese MedicineDepartment of Rehabilitation and Healthcare, Hunan University of MedicineZhongshan Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Chinese MedicineDongguan People’s hospitalSchool of Basic Medicine, Guangzhou University of Chinese MedicineSchool of Basic Medicine, Guangzhou University of Chinese MedicineFaculty of Chinese Medicine, State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, TaipaSchool of Basic Medicine, Guangzhou University of Chinese MedicineSchool of Basic Medicine, Guangzhou University of Chinese MedicineSchool of Basic Medicine, Guangzhou University of Chinese MedicineSchool of Basic Medicine, Guangzhou University of Chinese MedicineState Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou Medical UniversityDepartment of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of SingaporeSchool of Basic Medicine, Guangzhou University of Chinese MedicineAbstract High temperature and humidity in the environment are known to be associated with discomfort and disease, yet the underlying mechanisms remain unclear. We observed a decrease in plasma glucagon-like peptide-1 levels in response to high-temperature and humidity conditions. Through 16S rRNA gene sequencing, alterations in the gut microbiota composition were identified following exposure to high temperature and humidity conditions. Notably, changes in the gut microbiota have been implicated in bile acid synthesis. Further analysis revealed a decrease in lithocholic acid levels in high-temperature and humidity conditions. Subsequent in vitro experiments demonstrated that lithocholic acid increases glucagon-like peptide-1 secretion in NCI-H716 cells. Proteomic analysis indicated upregulation of farnesoid X receptor expression in the ileum. In vitro experiments revealed that the combination of lithocholic acid with farnesoid X receptor inhibitors resulted in a significant increase in GLP-1 levels compared to lithocholic acid alone. In this study, we elucidate the mechanism by which reduced lithocholic acid suppresses glucagon-like peptide 1 via farnesoid X receptor activation under high-temperature and humidity condition.https://doi.org/10.1038/s42003-024-06158-w |
| spellingShingle | Song Chen Zongren Hu Jianbang Tang Haipeng Zhu Yuhua Zheng Jiedong Xiao Youhua Xu Yao Wang Yi Luo Xiaoying Mo Yalan Wu Jianwen Guo Yongliang Zhang Huanhuan Luo High temperature and humidity in the environment disrupt bile acid metabolism, the gut microbiome, and GLP-1 secretion in mice Communications Biology |
| title | High temperature and humidity in the environment disrupt bile acid metabolism, the gut microbiome, and GLP-1 secretion in mice |
| title_full | High temperature and humidity in the environment disrupt bile acid metabolism, the gut microbiome, and GLP-1 secretion in mice |
| title_fullStr | High temperature and humidity in the environment disrupt bile acid metabolism, the gut microbiome, and GLP-1 secretion in mice |
| title_full_unstemmed | High temperature and humidity in the environment disrupt bile acid metabolism, the gut microbiome, and GLP-1 secretion in mice |
| title_short | High temperature and humidity in the environment disrupt bile acid metabolism, the gut microbiome, and GLP-1 secretion in mice |
| title_sort | high temperature and humidity in the environment disrupt bile acid metabolism the gut microbiome and glp 1 secretion in mice |
| url | https://doi.org/10.1038/s42003-024-06158-w |
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