Lipoteichoic Acid from <i>Lacticaseibacillus rhamnosus</i> GG Modulates Dendritic Cells and T Cells in the Gut
Lipoteichoic acid (LTA) from Gram-positive bacteria exerts different immune effects depending on the bacterial source from which it is isolated. <i>Lacticaseibacillus rhamnosus</i> GG LTA (LGG-LTA) oral administration reduces UVB-induced immunosuppression and skin tumor development in mi...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/3/723 |
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| author | Adrián D. Friedrich Juliana Leoni Mariela L. Paz Daniel H. González Maglio |
| author_facet | Adrián D. Friedrich Juliana Leoni Mariela L. Paz Daniel H. González Maglio |
| author_sort | Adrián D. Friedrich |
| collection | DOAJ |
| description | Lipoteichoic acid (LTA) from Gram-positive bacteria exerts different immune effects depending on the bacterial source from which it is isolated. <i>Lacticaseibacillus rhamnosus</i> GG LTA (LGG-LTA) oral administration reduces UVB-induced immunosuppression and skin tumor development in mice. In the present work, we evaluate the immunomodulatory effect exerted by LGG-LTA in dendritic cells (DC) and T cells, both in vitro and in the gut-associated lymphoid tissue (GALT). During cell culture, LTA-stimulated BMDC increased CD86 and MHC-II expression and secreted low levels of pro and anti-inflammatory cytokines. Moreover, LTA-treated BMDC increased T cell priming capacity, promoting the secretion of IL-17A. On the other hand, in orally LTA-treated mice, a decrease in mature DC (lamina propria and Peyer’s patches) was observed. Concomitantly, an increase in IL-12p35 and IFN-γ transcription was presented (lamina propria and Peyer’s Patches). Finally, an increase in the number of CD103+ DC was observed in Peyer’s patches. Together, our data demonstrate that LGG-LTA activates DC and T cells. Moreover, we show that a Th1-biased immune response is triggered in vivo after oral LTA administration. These effects justify the oral LTA activity previously observed. |
| first_indexed | 2024-03-09T23:20:45Z |
| format | Article |
| id | doaj.art-abdea0f1022446c388b84699e792e2a9 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T23:20:45Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-abdea0f1022446c388b84699e792e2a92023-11-23T17:28:33ZengMDPI AGNutrients2072-66432022-02-0114372310.3390/nu14030723Lipoteichoic Acid from <i>Lacticaseibacillus rhamnosus</i> GG Modulates Dendritic Cells and T Cells in the GutAdrián D. Friedrich0Juliana Leoni1Mariela L. Paz2Daniel H. González Maglio3Cátedra de Inmunología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires 1053, ArgentinaInstituto de Estudios de la Inmunidad Humoral (IDEHU), CONICET—Universidad de Buenos Aires, Buenos Aires 1053, ArgentinaCátedra de Inmunología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires 1053, ArgentinaCátedra de Inmunología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires 1053, ArgentinaLipoteichoic acid (LTA) from Gram-positive bacteria exerts different immune effects depending on the bacterial source from which it is isolated. <i>Lacticaseibacillus rhamnosus</i> GG LTA (LGG-LTA) oral administration reduces UVB-induced immunosuppression and skin tumor development in mice. In the present work, we evaluate the immunomodulatory effect exerted by LGG-LTA in dendritic cells (DC) and T cells, both in vitro and in the gut-associated lymphoid tissue (GALT). During cell culture, LTA-stimulated BMDC increased CD86 and MHC-II expression and secreted low levels of pro and anti-inflammatory cytokines. Moreover, LTA-treated BMDC increased T cell priming capacity, promoting the secretion of IL-17A. On the other hand, in orally LTA-treated mice, a decrease in mature DC (lamina propria and Peyer’s patches) was observed. Concomitantly, an increase in IL-12p35 and IFN-γ transcription was presented (lamina propria and Peyer’s Patches). Finally, an increase in the number of CD103+ DC was observed in Peyer’s patches. Together, our data demonstrate that LGG-LTA activates DC and T cells. Moreover, we show that a Th1-biased immune response is triggered in vivo after oral LTA administration. These effects justify the oral LTA activity previously observed.https://www.mdpi.com/2072-6643/14/3/723dendritic cells (DCs)probioticsTLR-ligandsT cells |
| spellingShingle | Adrián D. Friedrich Juliana Leoni Mariela L. Paz Daniel H. González Maglio Lipoteichoic Acid from <i>Lacticaseibacillus rhamnosus</i> GG Modulates Dendritic Cells and T Cells in the Gut Nutrients dendritic cells (DCs) probiotics TLR-ligands T cells |
| title | Lipoteichoic Acid from <i>Lacticaseibacillus rhamnosus</i> GG Modulates Dendritic Cells and T Cells in the Gut |
| title_full | Lipoteichoic Acid from <i>Lacticaseibacillus rhamnosus</i> GG Modulates Dendritic Cells and T Cells in the Gut |
| title_fullStr | Lipoteichoic Acid from <i>Lacticaseibacillus rhamnosus</i> GG Modulates Dendritic Cells and T Cells in the Gut |
| title_full_unstemmed | Lipoteichoic Acid from <i>Lacticaseibacillus rhamnosus</i> GG Modulates Dendritic Cells and T Cells in the Gut |
| title_short | Lipoteichoic Acid from <i>Lacticaseibacillus rhamnosus</i> GG Modulates Dendritic Cells and T Cells in the Gut |
| title_sort | lipoteichoic acid from i lacticaseibacillus rhamnosus i gg modulates dendritic cells and t cells in the gut |
| topic | dendritic cells (DCs) probiotics TLR-ligands T cells |
| url | https://www.mdpi.com/2072-6643/14/3/723 |
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