Neopterin as a Stimulator of Vascular Endothelial Growth Factor and Erythropoietin Gene Expression in an Ovarian Carcinoma Cell Line
Increases in serum neopterin concentrations are a regular finding in patients with ovarian cancer. The strong correlation between neopterin and tumor progression might be explained by a direct influence of the pteridine compound on tumor cell growth. In the present study we investigated whether neop...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2005-11-01
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| Series: | Pteridines |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/pteridines.2005.16.4.166 |
| _version_ | 1818584746485088256 |
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| author | Rieder Josef Marth Christian Schobersberger Wolfgang Hoffmann Georg |
| author_facet | Rieder Josef Marth Christian Schobersberger Wolfgang Hoffmann Georg |
| author_sort | Rieder Josef |
| collection | DOAJ |
| description | Increases in serum neopterin concentrations are a regular finding in patients with ovarian cancer. The strong correlation between neopterin and tumor progression might be explained by a direct influence of the pteridine compound on tumor cell growth. In the present study we investigated whether neopterin affects the production of the angiogenic promotors vascular endothelial growth factor (VEGF) and erythropoietin (EPO) in the human ovarian carcinoma cell line OVCAR-3. VEGF and EPO gene expression were detected via RT-PCR. and a possible involvement of hypoxiainducible factor-1 was assessed by electrophoretic mobility shift assays. Neopterin amplified the hypoxia-induced VEGF and EPO gene expression as well as protein synthesis in a concentration-dependent manner. In addition, generation of both VEGF and EPO could be detected following incubation of cells with neopterin under normoxic conditions, too. Neopterin-induced synthesis of VEGF and EPO was preceeded by a stimulation of hypoxiainducible factor-1 uptake into the nucleus of OVCAR-3 cells. From these data we conclude that neopterin acts as a mediator of angiogenesis. Stimulation of VEGF and EPO generation in ovarian carcinoma cells may promote vascularization of the malignant tissue and thus tumor growth and development. |
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| format | Article |
| id | doaj.art-abdfa3276a2b442c9b83a1e5b30bfdb7 |
| institution | Directory Open Access Journal |
| issn | 0933-4807 2195-4720 |
| language | English |
| last_indexed | 2024-12-16T08:26:04Z |
| publishDate | 2005-11-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Pteridines |
| spelling | doaj.art-abdfa3276a2b442c9b83a1e5b30bfdb72022-12-21T22:38:00ZengDe GruyterPteridines0933-48072195-47202005-11-0116416617310.1515/pteridines.2005.16.4.166Neopterin as a Stimulator of Vascular Endothelial Growth Factor and Erythropoietin Gene Expression in an Ovarian Carcinoma Cell LineRieder Josef0Marth Christian1Schobersberger Wolfgang2Hoffmann Georg3Department of Anaesthesia and Critical Care Medicine; Innsbruck Medical University, Innsbruck, AustriaDepartment of Obstetrics and Gynaecology; Innsbruck Medical University, Innsbruck, AustriaInstitute for Leisure, Travel, and Alpine Medicine; University for Health Sciences, Medical Informatics, and Technology, Hall, AustriaInstitute for Leisure, Travel, and Alpine Medicine; University for Health Sciences, Medical Informatics, and Technology, Hall, AustriaIncreases in serum neopterin concentrations are a regular finding in patients with ovarian cancer. The strong correlation between neopterin and tumor progression might be explained by a direct influence of the pteridine compound on tumor cell growth. In the present study we investigated whether neopterin affects the production of the angiogenic promotors vascular endothelial growth factor (VEGF) and erythropoietin (EPO) in the human ovarian carcinoma cell line OVCAR-3. VEGF and EPO gene expression were detected via RT-PCR. and a possible involvement of hypoxiainducible factor-1 was assessed by electrophoretic mobility shift assays. Neopterin amplified the hypoxia-induced VEGF and EPO gene expression as well as protein synthesis in a concentration-dependent manner. In addition, generation of both VEGF and EPO could be detected following incubation of cells with neopterin under normoxic conditions, too. Neopterin-induced synthesis of VEGF and EPO was preceeded by a stimulation of hypoxiainducible factor-1 uptake into the nucleus of OVCAR-3 cells. From these data we conclude that neopterin acts as a mediator of angiogenesis. Stimulation of VEGF and EPO generation in ovarian carcinoma cells may promote vascularization of the malignant tissue and thus tumor growth and development.https://doi.org/10.1515/pteridines.2005.16.4.166neopterinvascular endothelial growth factorerythropoietinovarian carcinoma cells |
| spellingShingle | Rieder Josef Marth Christian Schobersberger Wolfgang Hoffmann Georg Neopterin as a Stimulator of Vascular Endothelial Growth Factor and Erythropoietin Gene Expression in an Ovarian Carcinoma Cell Line Pteridines neopterin vascular endothelial growth factor erythropoietin ovarian carcinoma cells |
| title | Neopterin as a Stimulator of Vascular Endothelial Growth Factor and Erythropoietin Gene Expression in an Ovarian Carcinoma Cell Line |
| title_full | Neopterin as a Stimulator of Vascular Endothelial Growth Factor and Erythropoietin Gene Expression in an Ovarian Carcinoma Cell Line |
| title_fullStr | Neopterin as a Stimulator of Vascular Endothelial Growth Factor and Erythropoietin Gene Expression in an Ovarian Carcinoma Cell Line |
| title_full_unstemmed | Neopterin as a Stimulator of Vascular Endothelial Growth Factor and Erythropoietin Gene Expression in an Ovarian Carcinoma Cell Line |
| title_short | Neopterin as a Stimulator of Vascular Endothelial Growth Factor and Erythropoietin Gene Expression in an Ovarian Carcinoma Cell Line |
| title_sort | neopterin as a stimulator of vascular endothelial growth factor and erythropoietin gene expression in an ovarian carcinoma cell line |
| topic | neopterin vascular endothelial growth factor erythropoietin ovarian carcinoma cells |
| url | https://doi.org/10.1515/pteridines.2005.16.4.166 |
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