Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots
Abstract Background Alfalfa (Medicago sativa) is the most extensively cultivated forage legume in the world, and salinity stress is the most problematic environmental factors limiting alfalfa production. To evaluate alfalfa tissue variations in response to salt stress, comparative physiological and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-10-01
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| Series: | Proteome Science |
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| Online Access: | http://link.springer.com/article/10.1186/s12953-017-0127-z |
| _version_ | 1818259420903112704 |
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| author | Junbo Xiong Yan Sun Qingchuan Yang Hong Tian Heshan Zhang Yang Liu Mingxin Chen |
| author_facet | Junbo Xiong Yan Sun Qingchuan Yang Hong Tian Heshan Zhang Yang Liu Mingxin Chen |
| author_sort | Junbo Xiong |
| collection | DOAJ |
| description | Abstract Background Alfalfa (Medicago sativa) is the most extensively cultivated forage legume in the world, and salinity stress is the most problematic environmental factors limiting alfalfa production. To evaluate alfalfa tissue variations in response to salt stress, comparative physiological and proteomic analyses were made of salt responses in the roots and shoots of the alfalfa. Method A two-dimensional gel electrophoresis (2-DE)-based proteomic technique was employed to identify the differentially abundant proteins (DAPs) from salt-treated alfalfa roots and shoots of the salt tolerance cultivars Zhongmu No 1 cultivar, which was subjected to a range of salt stress concentrations for 9 days. In parallel, REL, MAD and H2O2 contents, and the activities of antioxidant enzymes of shoots and roots were determinand. Result Twenty-seven spots in the shoots and 36 spots in the roots that exhibited showed significant abundance variations were identified by MALDI-TOF-TOF MS. These DAPs are mainly involved in the biological processes of photosynthesis, stress and defense, carbohydrate and energy metabolism, second metabolism, protein metabolism, transcriptional regulation, cell wall and cytoskeleton metabolism, ion transpor, signal transduction. In parallel, physiological data were correlated well with our proteomic results. It is worth emphasizing that some novel salt-responsive proteins were identified, such as CP12, pathogenesis-related protein 2, harvest-induced protein, isoliquiritigenin 2′-O-methyltransferase. qRT-PCR was used to study the gene expression levels of the four above-mentioned proteins; four patterns are consistent with those of induced protein. Conclusion The primary mechanisms underlying the ability of alfalfa seedlings to tolerate salt stress were photosynthesis, detoxifying and antioxidant, secondary metabolism, and ion transport. And it also suggests that the different tissues responded to salt-stress in different ways. |
| first_indexed | 2024-12-12T18:15:10Z |
| format | Article |
| id | doaj.art-abe481c4b1bd4af3a86ce96b9ed74514 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-12-12T18:15:10Z |
| publishDate | 2017-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-abe481c4b1bd4af3a86ce96b9ed745142022-12-22T00:16:18ZengBMCProteome Science1477-59562017-10-0115111910.1186/s12953-017-0127-zProteomic analysis of early salt stress responsive proteins in alfalfa roots and shootsJunbo Xiong0Yan Sun1Qingchuan Yang2Hong Tian3Heshan Zhang4Yang Liu5Mingxin Chen6Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal and Veterinary Science, Hubei Academy of Agricultural ScienceInstitute of Grassland Science, China Agricultural UniversityInstitute of Animal Science, Chinese Academy of Agricultural ScienceHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal and Veterinary Science, Hubei Academy of Agricultural ScienceHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal and Veterinary Science, Hubei Academy of Agricultural ScienceHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal and Veterinary Science, Hubei Academy of Agricultural ScienceHubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal and Veterinary Science, Hubei Academy of Agricultural ScienceAbstract Background Alfalfa (Medicago sativa) is the most extensively cultivated forage legume in the world, and salinity stress is the most problematic environmental factors limiting alfalfa production. To evaluate alfalfa tissue variations in response to salt stress, comparative physiological and proteomic analyses were made of salt responses in the roots and shoots of the alfalfa. Method A two-dimensional gel electrophoresis (2-DE)-based proteomic technique was employed to identify the differentially abundant proteins (DAPs) from salt-treated alfalfa roots and shoots of the salt tolerance cultivars Zhongmu No 1 cultivar, which was subjected to a range of salt stress concentrations for 9 days. In parallel, REL, MAD and H2O2 contents, and the activities of antioxidant enzymes of shoots and roots were determinand. Result Twenty-seven spots in the shoots and 36 spots in the roots that exhibited showed significant abundance variations were identified by MALDI-TOF-TOF MS. These DAPs are mainly involved in the biological processes of photosynthesis, stress and defense, carbohydrate and energy metabolism, second metabolism, protein metabolism, transcriptional regulation, cell wall and cytoskeleton metabolism, ion transpor, signal transduction. In parallel, physiological data were correlated well with our proteomic results. It is worth emphasizing that some novel salt-responsive proteins were identified, such as CP12, pathogenesis-related protein 2, harvest-induced protein, isoliquiritigenin 2′-O-methyltransferase. qRT-PCR was used to study the gene expression levels of the four above-mentioned proteins; four patterns are consistent with those of induced protein. Conclusion The primary mechanisms underlying the ability of alfalfa seedlings to tolerate salt stress were photosynthesis, detoxifying and antioxidant, secondary metabolism, and ion transport. And it also suggests that the different tissues responded to salt-stress in different ways.http://link.springer.com/article/10.1186/s12953-017-0127-zNaCl stressMedicago sativa root and shootTwo-dimensional electrophoresisDifferentially abundant proteins |
| spellingShingle | Junbo Xiong Yan Sun Qingchuan Yang Hong Tian Heshan Zhang Yang Liu Mingxin Chen Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots Proteome Science NaCl stress Medicago sativa root and shoot Two-dimensional electrophoresis Differentially abundant proteins |
| title | Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots |
| title_full | Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots |
| title_fullStr | Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots |
| title_full_unstemmed | Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots |
| title_short | Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots |
| title_sort | proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots |
| topic | NaCl stress Medicago sativa root and shoot Two-dimensional electrophoresis Differentially abundant proteins |
| url | http://link.springer.com/article/10.1186/s12953-017-0127-z |
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