Research into the intervention effect of folic acid on arsenic-induced heart abnormalities in fetal rats during the periconception period
Abstract Background The incidence of CHD is the highest among birth defects and is increasing year to year. CHD seriously harms the health of infants and young children and presents a large economic burden to families and society. The pathogenesis of CHD and preventive measures are the focus of curr...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | BMC Cardiovascular Disorders |
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| Online Access: | http://link.springer.com/article/10.1186/s12872-020-01418-z |
| _version_ | 1818285310508793856 |
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| author | Lin Na Bai Q Zhao Xiumei Zhuang Lingzi He Deqin Zhuang Xuanxuan Guo Huanhuan Lin Yuan Chen Xiujuan |
| author_facet | Lin Na Bai Q Zhao Xiumei Zhuang Lingzi He Deqin Zhuang Xuanxuan Guo Huanhuan Lin Yuan Chen Xiujuan |
| author_sort | Lin Na |
| collection | DOAJ |
| description | Abstract Background The incidence of CHD is the highest among birth defects and is increasing year to year. CHD seriously harms the health of infants and young children and presents a large economic burden to families and society. The pathogenesis of CHD and preventive measures are the focus of current research. Our research aimed to explore the intervention effect of folic acid on heart abnormalities resulting from sodium arsenic (NaAsO2) exposure during the periconception period. Methods Sixty 35-day-old female SD rats were randomly divided into 5 groups with 12 rats in each group. Group A was the control group. The rats were given distilled water and ordinary chow. The rats in group B were given distilled water containing 75 mg/L NaAsO2 and ordinary chow. The rats in groups C, D, and E were given distilled water containing 75 mg/L NaAsO2 and chow containing 0.53 mg/kg, 5.3 mg/kg, and 10.6 mg/kg folic acid, respectively. The general condition of the embryos and the histopathology of the embryonic hearts were examined. The acetylation levels of histone H3K9 in heart tissues and the expression levels of Mef2C (which is related to heart development) were observed. Results The embryo weight and placental weight of groups B-E were significantly lower than those of group A (P < 0.05). The heart malformation rate of the fetal rats in groups B-E was significantly higher than that of the fetal rats in group A (P < 0.05). We found that the level of H3K9 acetylation in fetal rat cardiomyocytes in groups B-E was significantly higher than that in group A (P < 0.05) and that the level of H3K9 acetylation in groups C-E was lower than that in group B (P < 0.05). The mRNA level of Mef2C in fetal rat cardiomyocytes in group B-E was significantly higher than that in group A (P < 0.05), and the mRNA level of Mef2C in groups C-E was significantly lower than that in group B (P < 0.05). Conclusion Supplementation with folic acid during the periconception period can interfere with the toxic effects of arsenic on the heart. The mechanism may be that lowering the acetylation levels of histone H3K9 in heart tissues leads to decreased expression levels of Mef2C, which may play a protective role in heart development in fetal rats. |
| first_indexed | 2024-12-13T01:06:40Z |
| format | Article |
| id | doaj.art-abe604fa90554d3d8399e795ae96a11a |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-13T01:06:40Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cardiovascular Disorders |
| spelling | doaj.art-abe604fa90554d3d8399e795ae96a11a2022-12-22T00:04:32ZengBMCBMC Cardiovascular Disorders1471-22612020-03-012011710.1186/s12872-020-01418-zResearch into the intervention effect of folic acid on arsenic-induced heart abnormalities in fetal rats during the periconception periodLin Na0Bai Q1Zhao Xiumei2Zhuang Lingzi3He Deqin4Zhuang Xuanxuan5Guo Huanhuan6Lin Yuan7Chen Xiujuan8Department of the Prenatal Diagnosis Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fujian Key Laboratory for Prenatal Diagnosis and Birth DefectDepartment of the Prenatal Diagnosis Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fujian Key Laboratory for Prenatal Diagnosis and Birth DefectDepartment of the Prenatal Diagnosis Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fujian Key Laboratory for Prenatal Diagnosis and Birth DefectDepartment of the Prenatal Diagnosis Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fujian Key Laboratory for Prenatal Diagnosis and Birth DefectDepartment of the Prenatal Diagnosis Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fujian Key Laboratory for Prenatal Diagnosis and Birth DefectDepartment of the Prenatal Diagnosis Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fujian Key Laboratory for Prenatal Diagnosis and Birth DefectDepartment of the Prenatal Diagnosis Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fujian Key Laboratory for Prenatal Diagnosis and Birth DefectDepartment of Obstetrics and Gynecology, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical UniversityDepartment of Obstetrics and Gynecology, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical UniversityAbstract Background The incidence of CHD is the highest among birth defects and is increasing year to year. CHD seriously harms the health of infants and young children and presents a large economic burden to families and society. The pathogenesis of CHD and preventive measures are the focus of current research. Our research aimed to explore the intervention effect of folic acid on heart abnormalities resulting from sodium arsenic (NaAsO2) exposure during the periconception period. Methods Sixty 35-day-old female SD rats were randomly divided into 5 groups with 12 rats in each group. Group A was the control group. The rats were given distilled water and ordinary chow. The rats in group B were given distilled water containing 75 mg/L NaAsO2 and ordinary chow. The rats in groups C, D, and E were given distilled water containing 75 mg/L NaAsO2 and chow containing 0.53 mg/kg, 5.3 mg/kg, and 10.6 mg/kg folic acid, respectively. The general condition of the embryos and the histopathology of the embryonic hearts were examined. The acetylation levels of histone H3K9 in heart tissues and the expression levels of Mef2C (which is related to heart development) were observed. Results The embryo weight and placental weight of groups B-E were significantly lower than those of group A (P < 0.05). The heart malformation rate of the fetal rats in groups B-E was significantly higher than that of the fetal rats in group A (P < 0.05). We found that the level of H3K9 acetylation in fetal rat cardiomyocytes in groups B-E was significantly higher than that in group A (P < 0.05) and that the level of H3K9 acetylation in groups C-E was lower than that in group B (P < 0.05). The mRNA level of Mef2C in fetal rat cardiomyocytes in group B-E was significantly higher than that in group A (P < 0.05), and the mRNA level of Mef2C in groups C-E was significantly lower than that in group B (P < 0.05). Conclusion Supplementation with folic acid during the periconception period can interfere with the toxic effects of arsenic on the heart. The mechanism may be that lowering the acetylation levels of histone H3K9 in heart tissues leads to decreased expression levels of Mef2C, which may play a protective role in heart development in fetal rats.http://link.springer.com/article/10.1186/s12872-020-01418-zCongenital heart diseaseFolic acidArsenicHistone H3K9Mef2C |
| spellingShingle | Lin Na Bai Q Zhao Xiumei Zhuang Lingzi He Deqin Zhuang Xuanxuan Guo Huanhuan Lin Yuan Chen Xiujuan Research into the intervention effect of folic acid on arsenic-induced heart abnormalities in fetal rats during the periconception period BMC Cardiovascular Disorders Congenital heart disease Folic acid Arsenic Histone H3K9 Mef2C |
| title | Research into the intervention effect of folic acid on arsenic-induced heart abnormalities in fetal rats during the periconception period |
| title_full | Research into the intervention effect of folic acid on arsenic-induced heart abnormalities in fetal rats during the periconception period |
| title_fullStr | Research into the intervention effect of folic acid on arsenic-induced heart abnormalities in fetal rats during the periconception period |
| title_full_unstemmed | Research into the intervention effect of folic acid on arsenic-induced heart abnormalities in fetal rats during the periconception period |
| title_short | Research into the intervention effect of folic acid on arsenic-induced heart abnormalities in fetal rats during the periconception period |
| title_sort | research into the intervention effect of folic acid on arsenic induced heart abnormalities in fetal rats during the periconception period |
| topic | Congenital heart disease Folic acid Arsenic Histone H3K9 Mef2C |
| url | http://link.springer.com/article/10.1186/s12872-020-01418-z |
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