Effect of Hydroalcoholic Extract of Saffron on the Functions of Rat Peritoneal Macrophages
Background and purpose: Saffron (Crocus sativus) is a medicinal plant with anti-inflammatory effects. Macrophages are major cells that participate in inflammatory and immunity responses. The present study was performed to investigate the immunomodulatory effects of hydro-alcoholic extract of Saffron...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2018-04-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-9713-en.html |
| _version_ | 1797943797301116928 |
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| author | Narges Damya Shiva Khezri Meysam Abtahi Froushani |
| author_facet | Narges Damya Shiva Khezri Meysam Abtahi Froushani |
| author_sort | Narges Damya |
| collection | DOAJ |
| description | Background and purpose: Saffron (Crocus sativus) is a medicinal plant with anti-inflammatory effects. Macrophages are major cells that participate in inflammatory and immunity responses. The present study was performed to investigate the immunomodulatory effects of hydro-alcoholic extract of Saffron on rat peritoneal macrophages.
Materials and methods: This experimental study was conducted in two groups of male rats; an experimental and a control group. The animals in treatment group received hydro-alcoholic extract of Saffron for 4 constitutive weeks (400 mg/kg- daily, orally). The control rats received the same volume of distilled water. At the end of the study, macrophages were isolated from peritoneal cavity of rats and challenged with opsonized yeast. Then, the macrophages were evaluated for the rate of respiratory burst, nitric oxide production, and cytokine production using nitro-blue tetrazolium reduction assay, Griess test, and ELISA, respectively.
Results: The results of stimulation of macrophages showed a significant decrease in respiratory burst (P<0.001) and nitric oxide production (P<0.01) in macrophages isolated from treatment groups compared to that in control rats. Nevertheless, the results of phagocytosis assay and vitality did not show any significant differences between macrophages in controls and treatment group. Moreover, Saffron caused a significant increase in production of anti-inflammatory IL-10 by macrophages isolated from treatment group (P<0.01). The level of pro-inflammatory IL-12 did not alter significantly. But, the IL-12 to IL-10 ratio decreased significantly (P<0.01).
Conclusion: It seems that Saffron is a natural source to intervene the damages caused by inflammatory macrophages in immunopathological conditions. |
| first_indexed | 2024-04-10T20:29:53Z |
| format | Article |
| id | doaj.art-abe79a9437784683b047c0ad5492ba09 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:29:53Z |
| publishDate | 2018-04-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-abe79a9437784683b047c0ad5492ba092023-01-25T07:35:55ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792018-04-01281591422Effect of Hydroalcoholic Extract of Saffron on the Functions of Rat Peritoneal MacrophagesNarges Damya0Shiva Khezri1Meysam Abtahi Froushani2 Background and purpose: Saffron (Crocus sativus) is a medicinal plant with anti-inflammatory effects. Macrophages are major cells that participate in inflammatory and immunity responses. The present study was performed to investigate the immunomodulatory effects of hydro-alcoholic extract of Saffron on rat peritoneal macrophages. Materials and methods: This experimental study was conducted in two groups of male rats; an experimental and a control group. The animals in treatment group received hydro-alcoholic extract of Saffron for 4 constitutive weeks (400 mg/kg- daily, orally). The control rats received the same volume of distilled water. At the end of the study, macrophages were isolated from peritoneal cavity of rats and challenged with opsonized yeast. Then, the macrophages were evaluated for the rate of respiratory burst, nitric oxide production, and cytokine production using nitro-blue tetrazolium reduction assay, Griess test, and ELISA, respectively. Results: The results of stimulation of macrophages showed a significant decrease in respiratory burst (P<0.001) and nitric oxide production (P<0.01) in macrophages isolated from treatment groups compared to that in control rats. Nevertheless, the results of phagocytosis assay and vitality did not show any significant differences between macrophages in controls and treatment group. Moreover, Saffron caused a significant increase in production of anti-inflammatory IL-10 by macrophages isolated from treatment group (P<0.01). The level of pro-inflammatory IL-12 did not alter significantly. But, the IL-12 to IL-10 ratio decreased significantly (P<0.01). Conclusion: It seems that Saffron is a natural source to intervene the damages caused by inflammatory macrophages in immunopathological conditions.http://jmums.mazums.ac.ir/article-1-9713-en.htmlsaffron (crocus sativus)macrophagecytokinerat |
| spellingShingle | Narges Damya Shiva Khezri Meysam Abtahi Froushani Effect of Hydroalcoholic Extract of Saffron on the Functions of Rat Peritoneal Macrophages Journal of Mazandaran University of Medical Sciences saffron (crocus sativus) macrophage cytokine rat |
| title | Effect of Hydroalcoholic Extract of Saffron on the
Functions of Rat Peritoneal Macrophages |
| title_full | Effect of Hydroalcoholic Extract of Saffron on the
Functions of Rat Peritoneal Macrophages |
| title_fullStr | Effect of Hydroalcoholic Extract of Saffron on the
Functions of Rat Peritoneal Macrophages |
| title_full_unstemmed | Effect of Hydroalcoholic Extract of Saffron on the
Functions of Rat Peritoneal Macrophages |
| title_short | Effect of Hydroalcoholic Extract of Saffron on the
Functions of Rat Peritoneal Macrophages |
| title_sort | effect of hydroalcoholic extract of saffron on the functions of rat peritoneal macrophages |
| topic | saffron (crocus sativus) macrophage cytokine rat |
| url | http://jmums.mazums.ac.ir/article-1-9713-en.html |
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