Astragalus Polysaccharides/Chitosan Microspheres for Nasal Delivery: Preparation, Optimization, Characterization, and Pharmacodynamics
Chitosan (CTS) constitutes a promising area in treatment of nose-related diseases as a nasal drug delivery carrier. Astragalus polysaccharide (APS) significantly attenuates eosinophils and neutrophil-dominant airway inflammation, and it has a potential pharmaceutical application in the treatment of...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2020.00230/full |
| _version_ | 1818246820034248704 |
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| author | Saisai Wang Yuqing Sun Jingjing Zhang Xiaoming Cui Zhilu Xu Dejun Ding Dejun Ding Dejun Ding Limin Zhao Wentong Li Wentong Li Wentong Li Weifen Zhang Weifen Zhang Weifen Zhang |
| author_facet | Saisai Wang Yuqing Sun Jingjing Zhang Xiaoming Cui Zhilu Xu Dejun Ding Dejun Ding Dejun Ding Limin Zhao Wentong Li Wentong Li Wentong Li Weifen Zhang Weifen Zhang Weifen Zhang |
| author_sort | Saisai Wang |
| collection | DOAJ |
| description | Chitosan (CTS) constitutes a promising area in treatment of nose-related diseases as a nasal drug delivery carrier. Astragalus polysaccharide (APS) significantly attenuates eosinophils and neutrophil-dominant airway inflammation, and it has a potential pharmaceutical application in the treatment of severe asthma. The purpose of this work was to prepare APS/CTS microspheres intended for nasal drug delivery by the spray-drying method. The characteristics of APS/CTS microspheres were evaluated by a scanning electron microscope, Fourier transform infrared spectroscopy, differential scanning calorimetry, and in vitro drug release. The effect of APS/CTS microspheres on rats with allergic rhinitis (AR) was investigated by eosinophil and neutrophil counts in nasal lavage fluid. Results of SEM showed that microspheres were spherical and wrinkled. In vitro release showed that 67.48–93.76% APS was released from APS/CTS microspheres at pH 6.8 within 24 h. The effects showed that APS/CTS microspheres alleviated allergic symptoms and reduced eosinophils infiltration and the expression of interleukin-4 in the nasal mucosa tissue of rats that had no liver and kidney toxicity by hematoxylin-eosin staining observation. In conclusion, these results indicated that APS/CTS microspheres had excellent characteristics for the treatment of AR. |
| first_indexed | 2024-12-12T14:54:53Z |
| format | Article |
| id | doaj.art-abe883e347bf4c309b94fdec62709b2c |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-12T14:54:53Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-abe883e347bf4c309b94fdec62709b2c2022-12-22T00:20:55ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-03-011110.3389/fphar.2020.00230503691Astragalus Polysaccharides/Chitosan Microspheres for Nasal Delivery: Preparation, Optimization, Characterization, and PharmacodynamicsSaisai Wang0Yuqing Sun1Jingjing Zhang2Xiaoming Cui3Zhilu Xu4Dejun Ding5Dejun Ding6Dejun Ding7Limin Zhao8Wentong Li9Wentong Li10Wentong Li11Weifen Zhang12Weifen Zhang13Weifen Zhang14School of Pharmacy, Weifang Medical University, Weifang, ChinaDepartment of Otolaryngology, Head and Neck Surgery, Central Hospital of Zibo, Zibo, ChinaCollege of Basic Medical, Qingdao Binhai University, Qingdao, ChinaSchool of Pharmacy, Weifang Medical University, Weifang, ChinaSchool of Pharmacy, Weifang Medical University, Weifang, ChinaSchool of Pharmacy, Weifang Medical University, Weifang, ChinaShandong Engineering Research Center for Smart Materials and Regenerative Medicine, Weifang Medical University, Weifang, ChinaInstitute for Smart Materials and Regenerative Medicine, Weifang Medical University, Weifang, ChinaDepartment of Otolaryngology, Head and Neck Surgery, Affiliated Hospital of Weifang Medical University, Weifang, ChinaShandong Engineering Research Center for Smart Materials and Regenerative Medicine, Weifang Medical University, Weifang, ChinaInstitute for Smart Materials and Regenerative Medicine, Weifang Medical University, Weifang, ChinaDepartment of Pathology, Weifang Medical University, Weifang, ChinaSchool of Pharmacy, Weifang Medical University, Weifang, ChinaShandong Engineering Research Center for Smart Materials and Regenerative Medicine, Weifang Medical University, Weifang, ChinaInstitute for Smart Materials and Regenerative Medicine, Weifang Medical University, Weifang, ChinaChitosan (CTS) constitutes a promising area in treatment of nose-related diseases as a nasal drug delivery carrier. Astragalus polysaccharide (APS) significantly attenuates eosinophils and neutrophil-dominant airway inflammation, and it has a potential pharmaceutical application in the treatment of severe asthma. The purpose of this work was to prepare APS/CTS microspheres intended for nasal drug delivery by the spray-drying method. The characteristics of APS/CTS microspheres were evaluated by a scanning electron microscope, Fourier transform infrared spectroscopy, differential scanning calorimetry, and in vitro drug release. The effect of APS/CTS microspheres on rats with allergic rhinitis (AR) was investigated by eosinophil and neutrophil counts in nasal lavage fluid. Results of SEM showed that microspheres were spherical and wrinkled. In vitro release showed that 67.48–93.76% APS was released from APS/CTS microspheres at pH 6.8 within 24 h. The effects showed that APS/CTS microspheres alleviated allergic symptoms and reduced eosinophils infiltration and the expression of interleukin-4 in the nasal mucosa tissue of rats that had no liver and kidney toxicity by hematoxylin-eosin staining observation. In conclusion, these results indicated that APS/CTS microspheres had excellent characteristics for the treatment of AR.https://www.frontiersin.org/article/10.3389/fphar.2020.00230/fullChitosanAstragalus polysaccharidespray dryingmicrospheresnasal drug delivery |
| spellingShingle | Saisai Wang Yuqing Sun Jingjing Zhang Xiaoming Cui Zhilu Xu Dejun Ding Dejun Ding Dejun Ding Limin Zhao Wentong Li Wentong Li Wentong Li Weifen Zhang Weifen Zhang Weifen Zhang Astragalus Polysaccharides/Chitosan Microspheres for Nasal Delivery: Preparation, Optimization, Characterization, and Pharmacodynamics Frontiers in Pharmacology Chitosan Astragalus polysaccharide spray drying microspheres nasal drug delivery |
| title | Astragalus Polysaccharides/Chitosan Microspheres for Nasal Delivery: Preparation, Optimization, Characterization, and Pharmacodynamics |
| title_full | Astragalus Polysaccharides/Chitosan Microspheres for Nasal Delivery: Preparation, Optimization, Characterization, and Pharmacodynamics |
| title_fullStr | Astragalus Polysaccharides/Chitosan Microspheres for Nasal Delivery: Preparation, Optimization, Characterization, and Pharmacodynamics |
| title_full_unstemmed | Astragalus Polysaccharides/Chitosan Microspheres for Nasal Delivery: Preparation, Optimization, Characterization, and Pharmacodynamics |
| title_short | Astragalus Polysaccharides/Chitosan Microspheres for Nasal Delivery: Preparation, Optimization, Characterization, and Pharmacodynamics |
| title_sort | astragalus polysaccharides chitosan microspheres for nasal delivery preparation optimization characterization and pharmacodynamics |
| topic | Chitosan Astragalus polysaccharide spray drying microspheres nasal drug delivery |
| url | https://www.frontiersin.org/article/10.3389/fphar.2020.00230/full |
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