Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function.
Membrane-anchored mucins are present in the apical surface glycocalyx of mucosal epithelial cells, each mucosal epithelium having at least two of the mucins. The mucins have been ascribed barrier functions, but direct comparisons of their functions within the same epithelium have not been done. In a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4072602?pdf=render |
| _version_ | 1817986101928787968 |
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| author | Ilene K Gipson Sandra Spurr-Michaud Ann Tisdale Balaraj B Menon |
| author_facet | Ilene K Gipson Sandra Spurr-Michaud Ann Tisdale Balaraj B Menon |
| author_sort | Ilene K Gipson |
| collection | DOAJ |
| description | Membrane-anchored mucins are present in the apical surface glycocalyx of mucosal epithelial cells, each mucosal epithelium having at least two of the mucins. The mucins have been ascribed barrier functions, but direct comparisons of their functions within the same epithelium have not been done. In an epithelial cell line that expresses the membrane-anchored mucins, MUC1 and MUC16, the mucins were independently and stably knocked down using shRNA. Barrier functions tested included dye penetrance, bacterial adherence and invasion, transepithelial resistance, tight junction formation, and apical surface size. Knockdown of MUC16 decreased all barrier functions tested, causing increased dye penetrance and bacterial invasion, decreased transepithelial resistance, surprisingly, disruption of tight junctions, and greater apical surface cell area. Knockdown of MUC1 did not decrease barrier function, in fact, barrier to dye penetrance and bacterial invasion increased significantly. These data suggest that barrier functions of membrane-anchored mucins vary in the context of other membrane mucins, and MUC16 provides a major barrier when present. |
| first_indexed | 2024-04-14T00:06:05Z |
| format | Article |
| id | doaj.art-abe8891505df4103b8263c97bffaec8c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T00:06:05Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-abe8891505df4103b8263c97bffaec8c2022-12-22T02:23:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e10039310.1371/journal.pone.0100393Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function.Ilene K GipsonSandra Spurr-MichaudAnn TisdaleBalaraj B MenonMembrane-anchored mucins are present in the apical surface glycocalyx of mucosal epithelial cells, each mucosal epithelium having at least two of the mucins. The mucins have been ascribed barrier functions, but direct comparisons of their functions within the same epithelium have not been done. In an epithelial cell line that expresses the membrane-anchored mucins, MUC1 and MUC16, the mucins were independently and stably knocked down using shRNA. Barrier functions tested included dye penetrance, bacterial adherence and invasion, transepithelial resistance, tight junction formation, and apical surface size. Knockdown of MUC16 decreased all barrier functions tested, causing increased dye penetrance and bacterial invasion, decreased transepithelial resistance, surprisingly, disruption of tight junctions, and greater apical surface cell area. Knockdown of MUC1 did not decrease barrier function, in fact, barrier to dye penetrance and bacterial invasion increased significantly. These data suggest that barrier functions of membrane-anchored mucins vary in the context of other membrane mucins, and MUC16 provides a major barrier when present.http://europepmc.org/articles/PMC4072602?pdf=render |
| spellingShingle | Ilene K Gipson Sandra Spurr-Michaud Ann Tisdale Balaraj B Menon Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function. PLoS ONE |
| title | Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function. |
| title_full | Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function. |
| title_fullStr | Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function. |
| title_full_unstemmed | Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function. |
| title_short | Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function. |
| title_sort | comparison of the transmembrane mucins muc1 and muc16 in epithelial barrier function |
| url | http://europepmc.org/articles/PMC4072602?pdf=render |
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