Phage Display Preparation of Specific Polypeptides in Atherosclerotic Foam Cells
Atherosclerosis and related complications are the most common causes of death in modern societies. Macrophage-derived foam cells play critical roles in the initiation and progression of atherosclerosis. Effective, rapid, and instrument-independent detection in the early stage of chronic atherosclero...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/2/562 |
| _version_ | 1797496184271536128 |
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| author | Xiang Ji Dan Liu Feng Wu Yu Cen Lan Ma |
| author_facet | Xiang Ji Dan Liu Feng Wu Yu Cen Lan Ma |
| author_sort | Xiang Ji |
| collection | DOAJ |
| description | Atherosclerosis and related complications are the most common causes of death in modern societies. Macrophage-derived foam cells play critical roles in the initiation and progression of atherosclerosis. Effective, rapid, and instrument-independent detection in the early stage of chronic atherosclerosis progression could provide an opportunity for early intervention and treatment. Therefore, as a starting point, in this study, we aimed to isolate and prepare foam cell-specific polypeptides using a phage display platform. The six target polypeptides, which were acquired in this study, were evaluated by ELISA and showed strong specificity with foam cells. Streptavidin coupled quantum dots (QDs) were used as fluorescence developing agents, and images of biotin-modified polypeptides specifically binding with foam cells were clearly observed. The polypeptides obtained in this study could lay the foundation for developing a rapid detection kit for early atherosclerosis lesions and could provide new materials for research on the mechanisms of foam cell formation and the development of blocking drugs. |
| first_indexed | 2024-03-10T03:00:04Z |
| format | Article |
| id | doaj.art-abe9594a306b490fa0210ce5f48c15d2 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T03:00:04Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-abe9594a306b490fa0210ce5f48c15d22023-11-23T12:49:06ZengMDPI AGApplied Sciences2076-34172022-01-0112256210.3390/app12020562Phage Display Preparation of Specific Polypeptides in Atherosclerotic Foam CellsXiang Ji0Dan Liu1Feng Wu2Yu Cen3Lan Ma4Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaSchool of Health, Zhuhai College of Science and Technology, Zhuhai 519041, ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaAtherosclerosis and related complications are the most common causes of death in modern societies. Macrophage-derived foam cells play critical roles in the initiation and progression of atherosclerosis. Effective, rapid, and instrument-independent detection in the early stage of chronic atherosclerosis progression could provide an opportunity for early intervention and treatment. Therefore, as a starting point, in this study, we aimed to isolate and prepare foam cell-specific polypeptides using a phage display platform. The six target polypeptides, which were acquired in this study, were evaluated by ELISA and showed strong specificity with foam cells. Streptavidin coupled quantum dots (QDs) were used as fluorescence developing agents, and images of biotin-modified polypeptides specifically binding with foam cells were clearly observed. The polypeptides obtained in this study could lay the foundation for developing a rapid detection kit for early atherosclerosis lesions and could provide new materials for research on the mechanisms of foam cell formation and the development of blocking drugs.https://www.mdpi.com/2076-3417/12/2/562atherosclerosisfoam cellphage displayspecific polypeptide |
| spellingShingle | Xiang Ji Dan Liu Feng Wu Yu Cen Lan Ma Phage Display Preparation of Specific Polypeptides in Atherosclerotic Foam Cells Applied Sciences atherosclerosis foam cell phage display specific polypeptide |
| title | Phage Display Preparation of Specific Polypeptides in Atherosclerotic Foam Cells |
| title_full | Phage Display Preparation of Specific Polypeptides in Atherosclerotic Foam Cells |
| title_fullStr | Phage Display Preparation of Specific Polypeptides in Atherosclerotic Foam Cells |
| title_full_unstemmed | Phage Display Preparation of Specific Polypeptides in Atherosclerotic Foam Cells |
| title_short | Phage Display Preparation of Specific Polypeptides in Atherosclerotic Foam Cells |
| title_sort | phage display preparation of specific polypeptides in atherosclerotic foam cells |
| topic | atherosclerosis foam cell phage display specific polypeptide |
| url | https://www.mdpi.com/2076-3417/12/2/562 |
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