Determination of tissue contributions to the circulating lipid pool in cold exposure via systematic assessment of lipid profiles
Plasma lipid levels are altered in chronic conditions such as type 2 diabetes and cardiovascular disease as well as during acute stresses such as fasting and cold exposure. Advances in MS-based lipidomics have uncovered a complex plasma lipidome of more than 500 lipids that serve functional roles, i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752200030X |
| _version_ | 1828330912400539648 |
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| author | Raghav Jain Gina Wade Irene Ong Bhagirath Chaurasia Judith Simcox |
| author_facet | Raghav Jain Gina Wade Irene Ong Bhagirath Chaurasia Judith Simcox |
| author_sort | Raghav Jain |
| collection | DOAJ |
| description | Plasma lipid levels are altered in chronic conditions such as type 2 diabetes and cardiovascular disease as well as during acute stresses such as fasting and cold exposure. Advances in MS-based lipidomics have uncovered a complex plasma lipidome of more than 500 lipids that serve functional roles, including as energy substrates and signaling molecules. This plasma lipid pool is maintained through regulation of tissue production, secretion, and uptake. A major challenge in understanding the lipidome complexity is establishing the tissues of origin and uptake for various plasma lipids, which is valuable for determining lipid functions. Using cold exposure as an acute stress, we performed global lipidomics on plasma and in nine tissues that may contribute to the circulating lipid pool. We found that numerous species of plasma acylcarnitines (ACars) and ceramides (Cers) were significantly altered upon cold exposure. Through computational assessment, we identified the liver and brown adipose tissue as major contributors and consumers of circulating ACars, in agreement with our previous work. We further identified the kidney and intestine as novel contributors to the circulating ACar pool and validated these findings with gene expression analysis. Regression analysis also identified that the brown adipose tissue and kidney are interactors with the plasma Cer pool. Taken together, these studies provide an adaptable computational tool to assess tissue contribution to the plasma lipid pool. Our findings have further implications in understanding the function of plasma ACars and Cers, which are elevated in metabolic diseases. |
| first_indexed | 2024-04-13T20:46:26Z |
| format | Article |
| id | doaj.art-abeb8bd7884b49348a337564a5dab8c1 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-04-13T20:46:26Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-abeb8bd7884b49348a337564a5dab8c12022-12-22T02:30:41ZengElsevierJournal of Lipid Research0022-22752022-07-01637100197Determination of tissue contributions to the circulating lipid pool in cold exposure via systematic assessment of lipid profilesRaghav Jain0Gina Wade1Irene Ong2Bhagirath Chaurasia3Judith Simcox4Department of Biochemistry, University of Wisconsin-Madison, Wisconsin, USADepartment of Biochemistry, University of Wisconsin-Madison, Wisconsin, USADepartment of Biostatistics and Medical Informatics, University of Wisconsin School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, USADivision of Endocrinology, Department of Internal Medicine, Carver College of Medicine, Fraternal Order of Eagles Diabetes Research Center, University of Iowa, Iowa, USADepartment of Biochemistry, University of Wisconsin-Madison, Wisconsin, USA; For correspondence: Judith SimcoxPlasma lipid levels are altered in chronic conditions such as type 2 diabetes and cardiovascular disease as well as during acute stresses such as fasting and cold exposure. Advances in MS-based lipidomics have uncovered a complex plasma lipidome of more than 500 lipids that serve functional roles, including as energy substrates and signaling molecules. This plasma lipid pool is maintained through regulation of tissue production, secretion, and uptake. A major challenge in understanding the lipidome complexity is establishing the tissues of origin and uptake for various plasma lipids, which is valuable for determining lipid functions. Using cold exposure as an acute stress, we performed global lipidomics on plasma and in nine tissues that may contribute to the circulating lipid pool. We found that numerous species of plasma acylcarnitines (ACars) and ceramides (Cers) were significantly altered upon cold exposure. Through computational assessment, we identified the liver and brown adipose tissue as major contributors and consumers of circulating ACars, in agreement with our previous work. We further identified the kidney and intestine as novel contributors to the circulating ACar pool and validated these findings with gene expression analysis. Regression analysis also identified that the brown adipose tissue and kidney are interactors with the plasma Cer pool. Taken together, these studies provide an adaptable computational tool to assess tissue contribution to the plasma lipid pool. Our findings have further implications in understanding the function of plasma ACars and Cers, which are elevated in metabolic diseases.http://www.sciencedirect.com/science/article/pii/S002222752200030Xlipidomicsceramidescold exposureMSacylcarnitinesmetabolism |
| spellingShingle | Raghav Jain Gina Wade Irene Ong Bhagirath Chaurasia Judith Simcox Determination of tissue contributions to the circulating lipid pool in cold exposure via systematic assessment of lipid profiles Journal of Lipid Research lipidomics ceramides cold exposure MS acylcarnitines metabolism |
| title | Determination of tissue contributions to the circulating lipid pool in cold exposure via systematic assessment of lipid profiles |
| title_full | Determination of tissue contributions to the circulating lipid pool in cold exposure via systematic assessment of lipid profiles |
| title_fullStr | Determination of tissue contributions to the circulating lipid pool in cold exposure via systematic assessment of lipid profiles |
| title_full_unstemmed | Determination of tissue contributions to the circulating lipid pool in cold exposure via systematic assessment of lipid profiles |
| title_short | Determination of tissue contributions to the circulating lipid pool in cold exposure via systematic assessment of lipid profiles |
| title_sort | determination of tissue contributions to the circulating lipid pool in cold exposure via systematic assessment of lipid profiles |
| topic | lipidomics ceramides cold exposure MS acylcarnitines metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S002222752200030X |
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