Sterilization of sugar beet plant material, depending on the type of sterilizer, concentration and exposure of sterilization in vitro
The thesis being presented are results of researches regarding the selection of sterilizer, exposure of sterilization and its concentration in sugar beet hybrids when injected into the culture in vitro. The most effective sterilizing agent to enter micro engraftments in isolated culture is defined 0...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Uman National University of Horticulture
2015-12-01
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| Series: | Вісник Уманського національного університету садівництва |
| Online Access: | http://www.visnyk-unaus.udau.edu.ua/assets/files/articles/Buleten2015/Buleten12015/18.pdf |
| _version_ | 1818120420634656768 |
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| author | Polishchuk V. V. Karpuk L. M. Pushka I.M. Serzhuk O. P. Polishchuk T. V. |
| author_facet | Polishchuk V. V. Karpuk L. M. Pushka I.M. Serzhuk O. P. Polishchuk T. V. |
| author_sort | Polishchuk V. V. |
| collection | DOAJ |
| description | The thesis being presented are results of researches regarding the selection of sterilizer, exposure of sterilization and its concentration in sugar beet hybrids when injected into the culture in vitro. The most effective sterilizing agent to enter micro engraftments in isolated culture is defined 0.1% matched aqueous solution of dichloride mercury exposure for 15 minutes is established. The exit of sterile viable explants from which soon evolved the plants-regenerates is amounted up to 96%. It should be noted that the best sterilizer was the five percent solution of Septodor-forte, for which the increasing of exposure the sterilization from 10 to 20 minutes is obtained the exit of sterile material at 50-90% level. |
| first_indexed | 2024-12-11T05:25:49Z |
| format | Article |
| id | doaj.art-abed1673ea9140928ab1511b3e4d8e02 |
| institution | Directory Open Access Journal |
| issn | 2310-0478 2310-046X |
| language | English |
| last_indexed | 2024-12-11T05:25:49Z |
| publishDate | 2015-12-01 |
| publisher | Uman National University of Horticulture |
| record_format | Article |
| series | Вісник Уманського національного університету садівництва |
| spelling | doaj.art-abed1673ea9140928ab1511b3e4d8e022022-12-22T01:19:34ZengUman National University of HorticultureВісник Уманського національного університету садівництва2310-04782310-046X2015-12-0117176Sterilization of sugar beet plant material, depending on the type of sterilizer, concentration and exposure of sterilization in vitroPolishchuk V. V.0Karpuk L. M.1Pushka I.M.2Serzhuk O. P.3Polishchuk T. V.4Uman National University of HorticultureBila Tserkva National Agrarian UniversityUman National University of HorticultureUman National University of HorticultureInstitute of Bioenergy Crops and Sugar Beet NAAS of UkraineThe thesis being presented are results of researches regarding the selection of sterilizer, exposure of sterilization and its concentration in sugar beet hybrids when injected into the culture in vitro. The most effective sterilizing agent to enter micro engraftments in isolated culture is defined 0.1% matched aqueous solution of dichloride mercury exposure for 15 minutes is established. The exit of sterile viable explants from which soon evolved the plants-regenerates is amounted up to 96%. It should be noted that the best sterilizer was the five percent solution of Septodor-forte, for which the increasing of exposure the sterilization from 10 to 20 minutes is obtained the exit of sterile material at 50-90% level.http://www.visnyk-unaus.udau.edu.ua/assets/files/articles/Buleten2015/Buleten12015/18.pdf |
| spellingShingle | Polishchuk V. V. Karpuk L. M. Pushka I.M. Serzhuk O. P. Polishchuk T. V. Sterilization of sugar beet plant material, depending on the type of sterilizer, concentration and exposure of sterilization in vitro Вісник Уманського національного університету садівництва |
| title | Sterilization of sugar beet plant material, depending on the type of sterilizer, concentration and exposure of sterilization in vitro |
| title_full | Sterilization of sugar beet plant material, depending on the type of sterilizer, concentration and exposure of sterilization in vitro |
| title_fullStr | Sterilization of sugar beet plant material, depending on the type of sterilizer, concentration and exposure of sterilization in vitro |
| title_full_unstemmed | Sterilization of sugar beet plant material, depending on the type of sterilizer, concentration and exposure of sterilization in vitro |
| title_short | Sterilization of sugar beet plant material, depending on the type of sterilizer, concentration and exposure of sterilization in vitro |
| title_sort | sterilization of sugar beet plant material depending on the type of sterilizer concentration and exposure of sterilization in vitro |
| url | http://www.visnyk-unaus.udau.edu.ua/assets/files/articles/Buleten2015/Buleten12015/18.pdf |
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