MiRNA expression in the cartilage of patients with osteoarthritis
Abstract Background Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. Methods Forty tissue samples from 29 patients undergoing jo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-03-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13018-017-0542-y |
| _version_ | 1798024494738046976 |
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| author | Marta Kopańska Dariusz Szala Joanna Czech Natalia Gabło Krzysztof Gargasz Mateusz Trzeciak Izabela Zawlik Sławomir Snela |
| author_facet | Marta Kopańska Dariusz Szala Joanna Czech Natalia Gabło Krzysztof Gargasz Mateusz Trzeciak Izabela Zawlik Sławomir Snela |
| author_sort | Marta Kopańska |
| collection | DOAJ |
| description | Abstract Background Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. Methods Forty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction. Results Expression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group). Conclusions These findings may contribute to disease prevention and the development of therapeutic targets for OA. |
| first_indexed | 2024-04-11T18:03:20Z |
| format | Article |
| id | doaj.art-abf48768f6f8459ea083a6f7e89f51f6 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T18:03:20Z |
| publishDate | 2017-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-abf48768f6f8459ea083a6f7e89f51f62022-12-22T04:10:25ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2017-03-011211710.1186/s13018-017-0542-yMiRNA expression in the cartilage of patients with osteoarthritisMarta Kopańska0Dariusz Szala1Joanna Czech2Natalia Gabło3Krzysztof Gargasz4Mateusz Trzeciak5Izabela Zawlik6Sławomir Snela7Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, University of RzeszowClinical Orthopedics and Traumatology Department in Clinical Hospital 2Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, University of RzeszowLaboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, University of RzeszowData Analysis Laboratory, Centre for Innovative Research in Medical and Natural Sciences, Faculty of Medicine, University of RzeszowClinical Orthopedics and Traumatology Department in Clinical Hospital 2Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, University of RzeszowClinical Orthopedics and Traumatology Department in Clinical Hospital 2Abstract Background Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. Methods Forty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction. Results Expression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group). Conclusions These findings may contribute to disease prevention and the development of therapeutic targets for OA.http://link.springer.com/article/10.1186/s13018-017-0542-yOsteoarthritisCartilagemiRNAExpressionPathogenesisPatients |
| spellingShingle | Marta Kopańska Dariusz Szala Joanna Czech Natalia Gabło Krzysztof Gargasz Mateusz Trzeciak Izabela Zawlik Sławomir Snela MiRNA expression in the cartilage of patients with osteoarthritis Journal of Orthopaedic Surgery and Research Osteoarthritis Cartilage miRNA Expression Pathogenesis Patients |
| title | MiRNA expression in the cartilage of patients with osteoarthritis |
| title_full | MiRNA expression in the cartilage of patients with osteoarthritis |
| title_fullStr | MiRNA expression in the cartilage of patients with osteoarthritis |
| title_full_unstemmed | MiRNA expression in the cartilage of patients with osteoarthritis |
| title_short | MiRNA expression in the cartilage of patients with osteoarthritis |
| title_sort | mirna expression in the cartilage of patients with osteoarthritis |
| topic | Osteoarthritis Cartilage miRNA Expression Pathogenesis Patients |
| url | http://link.springer.com/article/10.1186/s13018-017-0542-y |
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