CD163 as a Biomarker in Colorectal Cancer: The Expression on Circulating Monocytes and Tumor-Associated Macrophages, and the Soluble Form in the Blood
The macrophage-associated molecule CD163 has been reported as a prognostic biomarker in different cancer types, but its role in colorectal cancer (CRC) is unclear. We studied CD163 in the tumor microenvironment and circulation of patients with CRC in relation to clinicopathological parameters. An en...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/16/5925 |
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| author | Daniëlle Krijgsman Natasja L. De Vries Morten N. Andersen Anni Skovbo Rob A.E.M. Tollenaar Holger J. Møller Marianne Hokland Peter J.K. Kuppen |
| author_facet | Daniëlle Krijgsman Natasja L. De Vries Morten N. Andersen Anni Skovbo Rob A.E.M. Tollenaar Holger J. Møller Marianne Hokland Peter J.K. Kuppen |
| author_sort | Daniëlle Krijgsman |
| collection | DOAJ |
| description | The macrophage-associated molecule CD163 has been reported as a prognostic biomarker in different cancer types, but its role in colorectal cancer (CRC) is unclear. We studied CD163 in the tumor microenvironment and circulation of patients with CRC in relation to clinicopathological parameters. An enzyme-linked immunosorbent assay (ELISA) was used to measure the serum sCD163 levels and multiparameter flow cytometry was used to study the peripheral blood monocytes and their CD163 expression in CRC patients (<i>N</i> = 78) and healthy donors (<i>N</i> = 50). The distribution of tumor-associated macrophages (TAMs) was studied in primary colorectal tumors with multiplex immunofluorescence. We showed that CRC patients with above-median sCD163 level had a shorter overall survival (OS, <i>p</i> = 0.035) as well as disease-free survival (DFS, <i>p</i> = 0.005). The above-median sCD163 remained significantly associated with a shorter DFS in the multivariate analysis (<i>p</i> = 0.049). Moreover, a shorter OS was observed in CRC patients with an above-median total monocyte percentage (<i>p</i> = 0.007). The number and phenotype of the stromal and intraepithelial TAMs in colorectal tumors were not associated with clinical outcome. In conclusion, sCD163 and monocytes in the circulation may be potential prognostic biomarkers in CRC patients, whereas TAMs in the tumor showed no association with clinical outcome. Thus, our results emphasize the importance of the innate systemic immune response in CRC disease progression. |
| first_indexed | 2024-03-10T17:16:30Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T17:16:30Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-abf8008d136448b99b3ef6cbc991edd72023-11-20T10:28:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012116592510.3390/ijms21165925CD163 as a Biomarker in Colorectal Cancer: The Expression on Circulating Monocytes and Tumor-Associated Macrophages, and the Soluble Form in the BloodDaniëlle Krijgsman0Natasja L. De Vries1Morten N. Andersen2Anni Skovbo3Rob A.E.M. Tollenaar4Holger J. Møller5Marianne Hokland6Peter J.K. Kuppen7Department of Surgery, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The NetherlandsDepartment of Surgery, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The NetherlandsDepartment of Biomedicine, Aarhus University, 8000 Aarhus, DenmarkDepartment of Biomedicine, Aarhus University, 8000 Aarhus, DenmarkDepartment of Surgery, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The NetherlandsDepartment of Clinical Biochemistry, Aarhus University Hospital, 8200 Aarhus, DenmarkDepartment of Biomedicine, Aarhus University, 8000 Aarhus, DenmarkDepartment of Surgery, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The NetherlandsThe macrophage-associated molecule CD163 has been reported as a prognostic biomarker in different cancer types, but its role in colorectal cancer (CRC) is unclear. We studied CD163 in the tumor microenvironment and circulation of patients with CRC in relation to clinicopathological parameters. An enzyme-linked immunosorbent assay (ELISA) was used to measure the serum sCD163 levels and multiparameter flow cytometry was used to study the peripheral blood monocytes and their CD163 expression in CRC patients (<i>N</i> = 78) and healthy donors (<i>N</i> = 50). The distribution of tumor-associated macrophages (TAMs) was studied in primary colorectal tumors with multiplex immunofluorescence. We showed that CRC patients with above-median sCD163 level had a shorter overall survival (OS, <i>p</i> = 0.035) as well as disease-free survival (DFS, <i>p</i> = 0.005). The above-median sCD163 remained significantly associated with a shorter DFS in the multivariate analysis (<i>p</i> = 0.049). Moreover, a shorter OS was observed in CRC patients with an above-median total monocyte percentage (<i>p</i> = 0.007). The number and phenotype of the stromal and intraepithelial TAMs in colorectal tumors were not associated with clinical outcome. In conclusion, sCD163 and monocytes in the circulation may be potential prognostic biomarkers in CRC patients, whereas TAMs in the tumor showed no association with clinical outcome. Thus, our results emphasize the importance of the innate systemic immune response in CRC disease progression.https://www.mdpi.com/1422-0067/21/16/5925colorectal cancerprognostic immune markersmonocytestumor-associated macrophagessoluble CD163regulatory T cells |
| spellingShingle | Daniëlle Krijgsman Natasja L. De Vries Morten N. Andersen Anni Skovbo Rob A.E.M. Tollenaar Holger J. Møller Marianne Hokland Peter J.K. Kuppen CD163 as a Biomarker in Colorectal Cancer: The Expression on Circulating Monocytes and Tumor-Associated Macrophages, and the Soluble Form in the Blood International Journal of Molecular Sciences colorectal cancer prognostic immune markers monocytes tumor-associated macrophages soluble CD163 regulatory T cells |
| title | CD163 as a Biomarker in Colorectal Cancer: The Expression on Circulating Monocytes and Tumor-Associated Macrophages, and the Soluble Form in the Blood |
| title_full | CD163 as a Biomarker in Colorectal Cancer: The Expression on Circulating Monocytes and Tumor-Associated Macrophages, and the Soluble Form in the Blood |
| title_fullStr | CD163 as a Biomarker in Colorectal Cancer: The Expression on Circulating Monocytes and Tumor-Associated Macrophages, and the Soluble Form in the Blood |
| title_full_unstemmed | CD163 as a Biomarker in Colorectal Cancer: The Expression on Circulating Monocytes and Tumor-Associated Macrophages, and the Soluble Form in the Blood |
| title_short | CD163 as a Biomarker in Colorectal Cancer: The Expression on Circulating Monocytes and Tumor-Associated Macrophages, and the Soluble Form in the Blood |
| title_sort | cd163 as a biomarker in colorectal cancer the expression on circulating monocytes and tumor associated macrophages and the soluble form in the blood |
| topic | colorectal cancer prognostic immune markers monocytes tumor-associated macrophages soluble CD163 regulatory T cells |
| url | https://www.mdpi.com/1422-0067/21/16/5925 |
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