Exploring Active Peptides with Antimicrobial Activity In Planta against <i>Xylella fastidiosa</i>
<i>Xylella fastidiosa (Xf)</i> is a xylem-limited quarantine plant bacterium and one of the most harmful agricultural pathogens across the world. Despite significant research efforts, neither a direct treatment nor an efficient strategy has yet been developed for combatting Xylella-assoc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/11/1685 |
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| author | Kaoutar El Handi Miloud Sabri Franco Valentini Angelo De Stradis El Hassan Achbani Majida Hafidi Maroun El Moujabber Toufic Elbeaino |
| author_facet | Kaoutar El Handi Miloud Sabri Franco Valentini Angelo De Stradis El Hassan Achbani Majida Hafidi Maroun El Moujabber Toufic Elbeaino |
| author_sort | Kaoutar El Handi |
| collection | DOAJ |
| description | <i>Xylella fastidiosa (Xf)</i> is a xylem-limited quarantine plant bacterium and one of the most harmful agricultural pathogens across the world. Despite significant research efforts, neither a direct treatment nor an efficient strategy has yet been developed for combatting Xylella-associated diseases. Antimicrobial peptides (AMPs) have been gaining interest as a promising sustainable tool to control pathogens due to their unique mechanism of action, broad spectrum of activity, and low environmental impact. In this study, we disclose the bioactivity of nine AMPs reported in the literature to be efficient against human and plant pathogen bacteria, i.e., <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Pseudomonas aeruginosa</i>, against <i>Xf,</i> through in vitro and in vivo experiments. Based on viable-quantitative PCR (v-qPCR), fluorescence microscopy (FM), optical density (OD), and transmission electron microscopy (TEM) assays, peptides Ascaphin-8 (GF19), DASamP1 (FF13), and DASamP2 (IL14) demonstrated the highest bactericidal and antibiofilm activities and were more efficient than the peptide PB178 (KL29), reported as one of the most potent AMPs against <i>Xf</i> at present. Furthermore, these AMPs showed low to no toxicity when tested on eukaryotic cells. In in planta tests, no <i>Xf</i> disease symptoms were noticed in <i>Nicotiana tabacum</i> plants treated with the AMPs 40 days post inoculation. This study highlighted the high antagonistic activity of newly tested AMP candidates against <i>Xf</i>, which could lead to the development of promising eco-friendly management of <i>Xf</i>-related diseases. |
| first_indexed | 2024-03-09T18:27:39Z |
| format | Article |
| id | doaj.art-abf8cf61084a4646a4bdf498a35d6186 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T18:27:39Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-abf8cf61084a4646a4bdf498a35d61862023-11-24T07:45:37ZengMDPI AGBiology2079-77372022-11-011111168510.3390/biology11111685Exploring Active Peptides with Antimicrobial Activity In Planta against <i>Xylella fastidiosa</i>Kaoutar El Handi0Miloud Sabri1Franco Valentini2Angelo De Stradis3El Hassan Achbani4Majida Hafidi5Maroun El Moujabber6Toufic Elbeaino7Laboratory of Plant Biotechnology and Valorization of Bio-Resources, Faculty of Sciences, Moulay Ismail University, Meknes 11201, MoroccoLaboratory of Phyto-Bacteriology and Biocontrol, Plant Protection Unit-National Institute of Agronomic Research INRA, Meknes 50000, MoroccoIstituto Agronomico Mediterraneo di Bari (CIHEAM-IAMB), Via Ceglie 9, 70010 Valenzano, BA, ItalyNational Research Council of Italy (CNR), Institute for Sustainable Plant Protection (IPSP), University of Bari, Via Amendola 165/A, 70126 Bari, BA, ItalyLaboratory of Phyto-Bacteriology and Biocontrol, Plant Protection Unit-National Institute of Agronomic Research INRA, Meknes 50000, MoroccoLaboratory of Plant Biotechnology and Valorization of Bio-Resources, Faculty of Sciences, Moulay Ismail University, Meknes 11201, MoroccoIstituto Agronomico Mediterraneo di Bari (CIHEAM-IAMB), Via Ceglie 9, 70010 Valenzano, BA, ItalyIstituto Agronomico Mediterraneo di Bari (CIHEAM-IAMB), Via Ceglie 9, 70010 Valenzano, BA, Italy<i>Xylella fastidiosa (Xf)</i> is a xylem-limited quarantine plant bacterium and one of the most harmful agricultural pathogens across the world. Despite significant research efforts, neither a direct treatment nor an efficient strategy has yet been developed for combatting Xylella-associated diseases. Antimicrobial peptides (AMPs) have been gaining interest as a promising sustainable tool to control pathogens due to their unique mechanism of action, broad spectrum of activity, and low environmental impact. In this study, we disclose the bioactivity of nine AMPs reported in the literature to be efficient against human and plant pathogen bacteria, i.e., <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Pseudomonas aeruginosa</i>, against <i>Xf,</i> through in vitro and in vivo experiments. Based on viable-quantitative PCR (v-qPCR), fluorescence microscopy (FM), optical density (OD), and transmission electron microscopy (TEM) assays, peptides Ascaphin-8 (GF19), DASamP1 (FF13), and DASamP2 (IL14) demonstrated the highest bactericidal and antibiofilm activities and were more efficient than the peptide PB178 (KL29), reported as one of the most potent AMPs against <i>Xf</i> at present. Furthermore, these AMPs showed low to no toxicity when tested on eukaryotic cells. In in planta tests, no <i>Xf</i> disease symptoms were noticed in <i>Nicotiana tabacum</i> plants treated with the AMPs 40 days post inoculation. This study highlighted the high antagonistic activity of newly tested AMP candidates against <i>Xf</i>, which could lead to the development of promising eco-friendly management of <i>Xf</i>-related diseases.https://www.mdpi.com/2079-7737/11/11/1685bactericidalv-qPCRfluorescence microscopytransmission electron microscopyin-vivo and in-vitro assays |
| spellingShingle | Kaoutar El Handi Miloud Sabri Franco Valentini Angelo De Stradis El Hassan Achbani Majida Hafidi Maroun El Moujabber Toufic Elbeaino Exploring Active Peptides with Antimicrobial Activity In Planta against <i>Xylella fastidiosa</i> Biology bactericidal v-qPCR fluorescence microscopy transmission electron microscopy in-vivo and in-vitro assays |
| title | Exploring Active Peptides with Antimicrobial Activity In Planta against <i>Xylella fastidiosa</i> |
| title_full | Exploring Active Peptides with Antimicrobial Activity In Planta against <i>Xylella fastidiosa</i> |
| title_fullStr | Exploring Active Peptides with Antimicrobial Activity In Planta against <i>Xylella fastidiosa</i> |
| title_full_unstemmed | Exploring Active Peptides with Antimicrobial Activity In Planta against <i>Xylella fastidiosa</i> |
| title_short | Exploring Active Peptides with Antimicrobial Activity In Planta against <i>Xylella fastidiosa</i> |
| title_sort | exploring active peptides with antimicrobial activity in planta against i xylella fastidiosa i |
| topic | bactericidal v-qPCR fluorescence microscopy transmission electron microscopy in-vivo and in-vitro assays |
| url | https://www.mdpi.com/2079-7737/11/11/1685 |
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