Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method
The aim of this study is to determine the optimum condition for biohydrogen production from sugarcane bagasse (SCB) hydrolysate using a central composite design and response surface methodology (RSM). SCB was hydrolyzed with 0.5% (v/v) sulfuric acid at 121 °C, 0.15 MPa for 60 min in an autoclave at...
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| Format: | Article |
| Language: | English |
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BMC
2016-09-01
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| Series: | Sustainable Environment Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468203916300322 |
| _version_ | 1811210165991505920 |
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| author | Suksaman Sangyoka Alissara Reungsang Chiu-Yue Lin |
| author_facet | Suksaman Sangyoka Alissara Reungsang Chiu-Yue Lin |
| author_sort | Suksaman Sangyoka |
| collection | DOAJ |
| description | The aim of this study is to determine the optimum condition for biohydrogen production from sugarcane bagasse (SCB) hydrolysate using a central composite design and response surface methodology (RSM). SCB was hydrolyzed with 0.5% (v/v) sulfuric acid at 121 °C, 0.15 MPa for 60 min in an autoclave at a solid to liquid ratio of 1:15 (g:mL). Heat-treated bacterium obtained from a hydrogen producing fermentor was used as the inoculum. The interaction of three factors, i.e., substrate concentration, substrate:buffer ratio and inoculum:substrate ratio on hydrogen production potential (P) were investigated. The results indicated that the substrate concentration, substrate:buffer ratio and inoculum:substrate ratio had a significant influence effect on P. An optimal condition was found at substrate concentration of 22.77 g-total sugar L−1, 4.31 substrate:buffer ratio, and 0.31 inoculum:substrate ratio resulted in a maximum P of 6980 mL H2 L−1. The confirmation experiment results indicated that optimum P was statistically significant, from the predicted value obtained by RSM which suggests that RSM could be efficiently used to optimize a biohydrogen production from SCB hydrolysate using mixed cultures. These results indicates that the SCB hemicellulose hydrolysate is suitable as a fermentation media for producing biohydrogen. This approach will add value to SCB by converting agricultural waste into a safe and clean form of energy. |
| first_indexed | 2024-04-12T04:50:27Z |
| format | Article |
| id | doaj.art-abfb8f5ee7e442b6a3253a16b39c237b |
| institution | Directory Open Access Journal |
| issn | 2468-2039 |
| language | English |
| last_indexed | 2024-04-12T04:50:27Z |
| publishDate | 2016-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Sustainable Environment Research |
| spelling | doaj.art-abfb8f5ee7e442b6a3253a16b39c237b2022-12-22T03:47:19ZengBMCSustainable Environment Research2468-20392016-09-0126523524210.1016/j.serj.2016.05.001Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical methodSuksaman Sangyoka0Alissara Reungsang1Chiu-Yue Lin2Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok 65000, ThailandDepartment of Biotechnology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Environmental Engineering and Science, Feng Chia University, Taichung 40724, TaiwanThe aim of this study is to determine the optimum condition for biohydrogen production from sugarcane bagasse (SCB) hydrolysate using a central composite design and response surface methodology (RSM). SCB was hydrolyzed with 0.5% (v/v) sulfuric acid at 121 °C, 0.15 MPa for 60 min in an autoclave at a solid to liquid ratio of 1:15 (g:mL). Heat-treated bacterium obtained from a hydrogen producing fermentor was used as the inoculum. The interaction of three factors, i.e., substrate concentration, substrate:buffer ratio and inoculum:substrate ratio on hydrogen production potential (P) were investigated. The results indicated that the substrate concentration, substrate:buffer ratio and inoculum:substrate ratio had a significant influence effect on P. An optimal condition was found at substrate concentration of 22.77 g-total sugar L−1, 4.31 substrate:buffer ratio, and 0.31 inoculum:substrate ratio resulted in a maximum P of 6980 mL H2 L−1. The confirmation experiment results indicated that optimum P was statistically significant, from the predicted value obtained by RSM which suggests that RSM could be efficiently used to optimize a biohydrogen production from SCB hydrolysate using mixed cultures. These results indicates that the SCB hemicellulose hydrolysate is suitable as a fermentation media for producing biohydrogen. This approach will add value to SCB by converting agricultural waste into a safe and clean form of energy.http://www.sciencedirect.com/science/article/pii/S2468203916300322BiohydrogenCentral composite design (CCD)Response surface methodology (RSM)Sugarcane bagasseHydrolysate |
| spellingShingle | Suksaman Sangyoka Alissara Reungsang Chiu-Yue Lin Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method Sustainable Environment Research Biohydrogen Central composite design (CCD) Response surface methodology (RSM) Sugarcane bagasse Hydrolysate |
| title | Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method |
| title_full | Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method |
| title_fullStr | Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method |
| title_full_unstemmed | Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method |
| title_short | Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method |
| title_sort | optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method |
| topic | Biohydrogen Central composite design (CCD) Response surface methodology (RSM) Sugarcane bagasse Hydrolysate |
| url | http://www.sciencedirect.com/science/article/pii/S2468203916300322 |
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