Range of metastrongylids (superfamily Metastrongyloidea) of public health and veterinary concern present in livers of the endemic lizard Gallotia galloti of Tenerife, Canary Islands, Spain
Abstract Background Endemic lizards of the genus Gallotia are of high ecological value to the terrestrial ecosystem of the archipelago of the Canary Islands, being potent seed spreaders as well as an important component of the diet of other vertebrates. The endemic lizard Gallotia galloti in Tenerif...
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| Format: | Article |
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BMC
2023-03-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-023-05653-z |
| _version_ | 1797865426760237056 |
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| author | Elena Izquierdo-Rodriguez Lucia Anettová Kristýna Hrazdilová Pilar Foronda David Modrý |
| author_facet | Elena Izquierdo-Rodriguez Lucia Anettová Kristýna Hrazdilová Pilar Foronda David Modrý |
| author_sort | Elena Izquierdo-Rodriguez |
| collection | DOAJ |
| description | Abstract Background Endemic lizards of the genus Gallotia are of high ecological value to the terrestrial ecosystem of the archipelago of the Canary Islands, being potent seed spreaders as well as an important component of the diet of other vertebrates. The endemic lizard Gallotia galloti in Tenerife has recently been reported to be a paratenic host of Angiostrongylus cantonensis, an invasive metastrongylid with zoonotic potential that is associated with rats as definitive hosts. However, microscopic examination of G. galloti tissue samples also revealed the presence of other metastrongylid larvae inside granulomas on the liver of this reptile. The aim of this study was to investigate the presence of helminths other than A. cantonensis in tissues of G. galloti from Tenerife. Methods A multiplex-nested PCR targeting the internal transcribed spacer 1 was designed that enabled the species-specific detection of A. cantonensis, Angiostrongylus vasorum, Aelurostrongylus abstrusus, Crenosoma striatum and Crenosoma vulpis. Liver samples from 39 G. galloti were analysed. Results Five metastrongylids were detected: A. cantonensis (15.4% of samples analysed), A. vasorum (5.1%), Ae. abstrusus (30.8%), C. striatum (30.8%) and undetermined metastrongylid sequences (12.8%). Co-infection was highly prevalent among the lizards which tested positive. Conclusions The study provides a new specific tool for the simultaneous detection of a range of metastrongylids of veterinary importance as well as new data on the circulation of metastrongylids in an ecosystem dominated by lizards. Graphical Abstract |
| first_indexed | 2024-04-09T23:08:38Z |
| format | Article |
| id | doaj.art-ac01bd2e5a3a42e385a044a3a5623d15 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-09T23:08:38Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-ac01bd2e5a3a42e385a044a3a5623d152023-03-22T10:33:21ZengBMCParasites & Vectors1756-33052023-03-011611710.1186/s13071-023-05653-zRange of metastrongylids (superfamily Metastrongyloidea) of public health and veterinary concern present in livers of the endemic lizard Gallotia galloti of Tenerife, Canary Islands, SpainElena Izquierdo-Rodriguez0Lucia Anettová1Kristýna Hrazdilová2Pilar Foronda3David Modrý4Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La LagunaDepartment of Botany and Zoology, Faculty of Science, Masaryk UniversityDepartment of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in BrnoInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La LagunaDepartment of Botany and Zoology, Faculty of Science, Masaryk UniversityAbstract Background Endemic lizards of the genus Gallotia are of high ecological value to the terrestrial ecosystem of the archipelago of the Canary Islands, being potent seed spreaders as well as an important component of the diet of other vertebrates. The endemic lizard Gallotia galloti in Tenerife has recently been reported to be a paratenic host of Angiostrongylus cantonensis, an invasive metastrongylid with zoonotic potential that is associated with rats as definitive hosts. However, microscopic examination of G. galloti tissue samples also revealed the presence of other metastrongylid larvae inside granulomas on the liver of this reptile. The aim of this study was to investigate the presence of helminths other than A. cantonensis in tissues of G. galloti from Tenerife. Methods A multiplex-nested PCR targeting the internal transcribed spacer 1 was designed that enabled the species-specific detection of A. cantonensis, Angiostrongylus vasorum, Aelurostrongylus abstrusus, Crenosoma striatum and Crenosoma vulpis. Liver samples from 39 G. galloti were analysed. Results Five metastrongylids were detected: A. cantonensis (15.4% of samples analysed), A. vasorum (5.1%), Ae. abstrusus (30.8%), C. striatum (30.8%) and undetermined metastrongylid sequences (12.8%). Co-infection was highly prevalent among the lizards which tested positive. Conclusions The study provides a new specific tool for the simultaneous detection of a range of metastrongylids of veterinary importance as well as new data on the circulation of metastrongylids in an ecosystem dominated by lizards. Graphical Abstracthttps://doi.org/10.1186/s13071-023-05653-zGallotia gallotiAngiostrongylus cantonensisAngiostrongylus vasorumAelurostrongylus abstrususCrenosoma striatumMultiplex PCR |
| spellingShingle | Elena Izquierdo-Rodriguez Lucia Anettová Kristýna Hrazdilová Pilar Foronda David Modrý Range of metastrongylids (superfamily Metastrongyloidea) of public health and veterinary concern present in livers of the endemic lizard Gallotia galloti of Tenerife, Canary Islands, Spain Parasites & Vectors Gallotia galloti Angiostrongylus cantonensis Angiostrongylus vasorum Aelurostrongylus abstrusus Crenosoma striatum Multiplex PCR |
| title | Range of metastrongylids (superfamily Metastrongyloidea) of public health and veterinary concern present in livers of the endemic lizard Gallotia galloti of Tenerife, Canary Islands, Spain |
| title_full | Range of metastrongylids (superfamily Metastrongyloidea) of public health and veterinary concern present in livers of the endemic lizard Gallotia galloti of Tenerife, Canary Islands, Spain |
| title_fullStr | Range of metastrongylids (superfamily Metastrongyloidea) of public health and veterinary concern present in livers of the endemic lizard Gallotia galloti of Tenerife, Canary Islands, Spain |
| title_full_unstemmed | Range of metastrongylids (superfamily Metastrongyloidea) of public health and veterinary concern present in livers of the endemic lizard Gallotia galloti of Tenerife, Canary Islands, Spain |
| title_short | Range of metastrongylids (superfamily Metastrongyloidea) of public health and veterinary concern present in livers of the endemic lizard Gallotia galloti of Tenerife, Canary Islands, Spain |
| title_sort | range of metastrongylids superfamily metastrongyloidea of public health and veterinary concern present in livers of the endemic lizard gallotia galloti of tenerife canary islands spain |
| topic | Gallotia galloti Angiostrongylus cantonensis Angiostrongylus vasorum Aelurostrongylus abstrusus Crenosoma striatum Multiplex PCR |
| url | https://doi.org/10.1186/s13071-023-05653-z |
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