Cardioprotective Effect of Garlic Juice on the Isolated Rat Heart in Ischemia- Reperfusion
Background: It has been reported that the chronic oral administration of garlic homogenate protected the rat heart from in vitro ischemic reperfusion injury. However, the biological effects of garlic juice on the heart are expected to be different from oral administration of it. Objective: the prese...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2010-09-01
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| Series: | Journal of Medicinal Plants |
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| Online Access: | http://jmp.ir/article-1-268-en.html |
| _version_ | 1818360121237962752 |
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| author | D Shackebaei S Ghazvineh A Godini A Pilehvarian S Reshadat |
| author_facet | D Shackebaei S Ghazvineh A Godini A Pilehvarian S Reshadat |
| author_sort | D Shackebaei |
| collection | DOAJ |
| description | Background: It has been reported that the chronic oral administration of garlic homogenate protected the rat heart from in vitro ischemic reperfusion injury. However, the biological effects of garlic juice on the heart are expected to be different from oral administration of it. Objective: the present study was designed to investigate the effect of garlic juice on the isolated rat heart in ischemia- reperfusion. Methods: Rat isolated, perfused hearts were subjected to 30 min baseline measurement followed by 40 min normothermic global ischemia and 45 min reperfusion. Garlic juice (0.01 mg/ml) was added to the perfusion solution 20 min before ischemia in the test 1 and 5 min before and 10 min after ischemia in test 2. Different cardiac variables including left ventricular developed pressure (LVDP), heart rate (HR) and coronary flow (CF) were measured. Rate pressure product (RPP) was calculated, and released lactate dehydrogenase (LDH) enzyme in effluent was measured in reperfusion. Results: Garlic juice significantly increased CF before ischemia in both test groups. The released LDH enzyme at the first minute and the recovery of RPP and LVDP on the 45th minute of reperfusion were significantly better in the test group 2 in comparison to the control. Conclusion: The result of the present study shows that garlic juice has a vasodilator activity and protects the isolated ischemic rat heart when it was administrated in reperfusion. It is probably mediated by antioxidant activity of allicin as a principal bioactive compound of garlic juice. |
| first_indexed | 2024-12-13T20:55:45Z |
| format | Article |
| id | doaj.art-ac0b09fbc7b0415c88e86da610018d69 |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-13T20:55:45Z |
| publishDate | 2010-09-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-ac0b09fbc7b0415c88e86da610018d692022-12-21T23:31:46ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582010-09-019357179Cardioprotective Effect of Garlic Juice on the Isolated Rat Heart in Ischemia- ReperfusionD Shackebaei0S Ghazvineh1A Godini2A Pilehvarian3S Reshadat4 Medical Biology Research Center, Kermanshah University of Medical Sciences Medical Biology Research Center, Kermanshah University of Medical Sciences Medical Biology Research Center, Kermanshah University of Medical Sciences Department of Biology, School of Basic sciences, Payame Noor University of Esfahan Department of Paediatric, School of Medicine, Kermanshah University of Medical Sciences Background: It has been reported that the chronic oral administration of garlic homogenate protected the rat heart from in vitro ischemic reperfusion injury. However, the biological effects of garlic juice on the heart are expected to be different from oral administration of it. Objective: the present study was designed to investigate the effect of garlic juice on the isolated rat heart in ischemia- reperfusion. Methods: Rat isolated, perfused hearts were subjected to 30 min baseline measurement followed by 40 min normothermic global ischemia and 45 min reperfusion. Garlic juice (0.01 mg/ml) was added to the perfusion solution 20 min before ischemia in the test 1 and 5 min before and 10 min after ischemia in test 2. Different cardiac variables including left ventricular developed pressure (LVDP), heart rate (HR) and coronary flow (CF) were measured. Rate pressure product (RPP) was calculated, and released lactate dehydrogenase (LDH) enzyme in effluent was measured in reperfusion. Results: Garlic juice significantly increased CF before ischemia in both test groups. The released LDH enzyme at the first minute and the recovery of RPP and LVDP on the 45th minute of reperfusion were significantly better in the test group 2 in comparison to the control. Conclusion: The result of the present study shows that garlic juice has a vasodilator activity and protects the isolated ischemic rat heart when it was administrated in reperfusion. It is probably mediated by antioxidant activity of allicin as a principal bioactive compound of garlic juice.http://jmp.ir/article-1-268-en.htmlisolated rat heartgarlic juicemyocardial functionischemia-reperfusion |
| spellingShingle | D Shackebaei S Ghazvineh A Godini A Pilehvarian S Reshadat Cardioprotective Effect of Garlic Juice on the Isolated Rat Heart in Ischemia- Reperfusion Journal of Medicinal Plants isolated rat heart garlic juice myocardial function ischemia-reperfusion |
| title | Cardioprotective Effect of Garlic Juice on the Isolated Rat Heart in Ischemia- Reperfusion |
| title_full | Cardioprotective Effect of Garlic Juice on the Isolated Rat Heart in Ischemia- Reperfusion |
| title_fullStr | Cardioprotective Effect of Garlic Juice on the Isolated Rat Heart in Ischemia- Reperfusion |
| title_full_unstemmed | Cardioprotective Effect of Garlic Juice on the Isolated Rat Heart in Ischemia- Reperfusion |
| title_short | Cardioprotective Effect of Garlic Juice on the Isolated Rat Heart in Ischemia- Reperfusion |
| title_sort | cardioprotective effect of garlic juice on the isolated rat heart in ischemia reperfusion |
| topic | isolated rat heart garlic juice myocardial function ischemia-reperfusion |
| url | http://jmp.ir/article-1-268-en.html |
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