Marked variations in diversity and functions of gut microbiota between wild and domestic stag beetle Dorcus Hopei Hopei
Abstract Background Although stag beetles are a popular saprophytic insect, their gut microbiome has been poorly studied. Here, 16 S rRNA gene sequencing was employed to reveal the gut microbiota composition and functional variations between wild and domestic Dorcus hopei hopei (Dhh) larval individu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-023-03177-1 |
| _version_ | 1827377469796122624 |
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| author | Yikai Lu Siyuan Chu Zhiyuan Shi Ruobing You Haimin Chen |
| author_facet | Yikai Lu Siyuan Chu Zhiyuan Shi Ruobing You Haimin Chen |
| author_sort | Yikai Lu |
| collection | DOAJ |
| description | Abstract Background Although stag beetles are a popular saprophytic insect, their gut microbiome has been poorly studied. Here, 16 S rRNA gene sequencing was employed to reveal the gut microbiota composition and functional variations between wild and domestic Dorcus hopei hopei (Dhh) larval individuals. Results The results indicated a significant difference between the wild and domestic Dhh gut microbiota., the domestic Dhh individuals contained more gut microbial taxa (e.g. genera Ralstonia and Methyloversatilis) with xenobiotic degrading functions. The wild Dhh possesses gut microbiota compositions (e.g. Turicibacter and Tyzzerella ) more appropriate for energy metabolism and potential growth. This study furthermore assigned all Dhh individuals by size into groups for data analysis; which indicated limited disparities between the gut microbiota of different-sized D. hopei hopei larvae. Conclusion The outcome of this study illustrated that there exists a significant discrepancy in gut microbiota composition between wild and domestic Dhh larvae. In addition, the assemblage of gut microbiome in Dhh was primarily attributed to environmental influences instead of individual differences such as developmental potential or size. These findings will provide a valuable theoretical foundation for the protection of wild saprophytic insects and the potential utilization of the insect-associated intestinal microbiome in the future. |
| first_indexed | 2024-03-08T12:40:38Z |
| format | Article |
| id | doaj.art-ac0ef6b4828b45059bcb2b6afc59d5ee |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-08T12:40:38Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-ac0ef6b4828b45059bcb2b6afc59d5ee2024-01-21T12:12:45ZengBMCBMC Microbiology1471-21802024-01-0124111510.1186/s12866-023-03177-1Marked variations in diversity and functions of gut microbiota between wild and domestic stag beetle Dorcus Hopei HopeiYikai Lu0Siyuan Chu1Zhiyuan Shi2Ruobing You3Haimin Chen4BASIS International School HangzhouKey Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityBASIS International School HangzhouKey Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityKey Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityAbstract Background Although stag beetles are a popular saprophytic insect, their gut microbiome has been poorly studied. Here, 16 S rRNA gene sequencing was employed to reveal the gut microbiota composition and functional variations between wild and domestic Dorcus hopei hopei (Dhh) larval individuals. Results The results indicated a significant difference between the wild and domestic Dhh gut microbiota., the domestic Dhh individuals contained more gut microbial taxa (e.g. genera Ralstonia and Methyloversatilis) with xenobiotic degrading functions. The wild Dhh possesses gut microbiota compositions (e.g. Turicibacter and Tyzzerella ) more appropriate for energy metabolism and potential growth. This study furthermore assigned all Dhh individuals by size into groups for data analysis; which indicated limited disparities between the gut microbiota of different-sized D. hopei hopei larvae. Conclusion The outcome of this study illustrated that there exists a significant discrepancy in gut microbiota composition between wild and domestic Dhh larvae. In addition, the assemblage of gut microbiome in Dhh was primarily attributed to environmental influences instead of individual differences such as developmental potential or size. These findings will provide a valuable theoretical foundation for the protection of wild saprophytic insects and the potential utilization of the insect-associated intestinal microbiome in the future.https://doi.org/10.1186/s12866-023-03177-1Dorcus Hopei HopeiHabitat ecologyGut microbiota16S rRNA gene sequencingBioinformatics |
| spellingShingle | Yikai Lu Siyuan Chu Zhiyuan Shi Ruobing You Haimin Chen Marked variations in diversity and functions of gut microbiota between wild and domestic stag beetle Dorcus Hopei Hopei BMC Microbiology Dorcus Hopei Hopei Habitat ecology Gut microbiota 16S rRNA gene sequencing Bioinformatics |
| title | Marked variations in diversity and functions of gut microbiota between wild and domestic stag beetle Dorcus Hopei Hopei |
| title_full | Marked variations in diversity and functions of gut microbiota between wild and domestic stag beetle Dorcus Hopei Hopei |
| title_fullStr | Marked variations in diversity and functions of gut microbiota between wild and domestic stag beetle Dorcus Hopei Hopei |
| title_full_unstemmed | Marked variations in diversity and functions of gut microbiota between wild and domestic stag beetle Dorcus Hopei Hopei |
| title_short | Marked variations in diversity and functions of gut microbiota between wild and domestic stag beetle Dorcus Hopei Hopei |
| title_sort | marked variations in diversity and functions of gut microbiota between wild and domestic stag beetle dorcus hopei hopei |
| topic | Dorcus Hopei Hopei Habitat ecology Gut microbiota 16S rRNA gene sequencing Bioinformatics |
| url | https://doi.org/10.1186/s12866-023-03177-1 |
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